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- W4213371640 abstract "Allo-antibodies produced by K-negative pregnant women against a fetal K antigen from the Kell blood group system may cause hemolytic disease of the fetus and newborn (HDFN). Predicting the fetal K antigen using noninvasive prenatal testing (NIPT) is important for decisions concerning management of pregnancies. Digital and droplet digital PCR techniques permit the detection of fetal single nucleotide variant with a higher specificity and sensitivity than real-time polymerase chain reaction (PCR).The aim was to evaluate and compare protocols for fetal KEL*01.01 genotyping using different assays and digital PCR platforms.DNA isolated from 59 pregnant women (9-39 weeks of gestation, 49 with anti-K) was tested using home-made and custom-ordered KEL*01.01/KEL*02 assays with Droplet Digital™ and QuantStudio™3D. The results were compared with fetal/neonatal genotypes/phenotypes.Fetal KEL*01.01 results using all tested protocols were concordant with fetal/neonatal KEL*01.01 genotypes/phenotypes. None of the tested combinations of assays or digital PCR platforms gave false KEL*01.01-negative results, but inconclusive KEL*01.01 reads were observed in all tested protocols. For 36 cases compared using two digital PCR platforms and assays, there were not statistically significant differences in a level of fetal KEL*01.01 fraction (p < .72).Independent of the applied dPCR and ddPCR platforms and KEL*01.01 assays, prediction of the fetal KEL*01.01 is highly reliable. Before implementation in routine practice further validation of the KEL*01.01 protocol with a larger group of pregnant women should be performed." @default.
- W4213371640 created "2022-02-24" @default.
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- W4213371640 date "2022-02-22" @default.
- W4213371640 modified "2023-09-27" @default.
- W4213371640 title "Noninvasive diagnostics of fetal <i><scp>KEL</scp>*01.01</i> allele from maternal plasma of immunized women using digital <scp>PCR</scp> protocols" @default.
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- W4213371640 doi "https://doi.org/10.1111/trf.16829" @default.
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