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• W4214489096 endingPage "143" @default.
• W4214489096 startingPage "130" @default.
• W4214489096 abstract "Polymerase chain reaction is an important tool in molecular biology. Although the principles of the technique are relatively simple, amplifying complex or long DNA segments can be challenging. A variety of PCR additives are used to improve the performance and yield of difficult PCRs. Each PCR additive has unique properties and enhances PCR through a different mode of action. They are used to either improve PCR sensitivity, efficiency, and specificity, or mitigate the effects of PCR inhibitors. In this review, we categorise known PCR additives into four main groups. The first three groups comprise PCR additives with well-defined mechanisms, namely those that facilitate the amplification of GC-rich sequences, counteract the detrimental effects of PCR inhibitors, or alter PCR kinetics (nanomaterials). The fourth group is a loose mix of additives with unclear mechanisms of action. Then, we discuss how these additives may be used to tackle specific PCR-related challenges, particularly those associated with long-range PCR. We conclude the review with added insights into the use of PCR additives in enhancing the synthesis of complex and long DNA fragments." @default.
• W4214489096 created "2022-03-02" @default.
• W4214489096 creator A5007194424 @default.
• W4214489096 creator A5023469862 @default.
• W4214489096 creator A5058827919 @default.
• W4214489096 creator A5064880835 @default.
• W4214489096 creator A5069074647 @default.
• W4214489096 date "2022-06-01" @default.
• W4214489096 modified "2023-10-16" @default.
• W4214489096 title "PCR enhancers: Types, mechanisms, and applications in long-range PCR" @default.
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