Matches in SemOpenAlex for { <https://semopenalex.org/work/W4214532250> ?p ?o ?g. }
- W4214532250 endingPage "e0264130" @default.
- W4214532250 startingPage "e0264130" @default.
- W4214532250 abstract "The global COVID-19 pandemic has highlighted the need for rapid, accurate and accessible nucleic acid tests to enable timely identification of infected individuals. We optimized a sample-to-answer nucleic acid test for SARS-CoV-2 that provides results in <1 hour using inexpensive and readily available reagents. The test workflow includes a simple lysis and viral inactivation protocol followed by direct isothermal amplification of viral RNA using RT-LAMP. The assay was validated using two different instruments, a portable isothermal fluorimeter and a standard thermocycler. Results of the RT-LAMP assay were compared to traditional RT-qPCR for nasopharyngeal swabs, nasal swabs, and saliva collected from a cohort of patients hospitalized due to COVID-19. For all three sample types, positive agreement with RT-LAMP performed using the isothermal fluorimeter was 100% for samples with Ct <30 and 69-91% for samples with Ct <40. Following validation, the test was successfully scaled to test the saliva of up to 400 asymptomatic individuals per day as part of the campus surveillance program at Rice University. Successful development, validation, and scaling of this sample-to-answer, extraction-free real-time RT-LAMP test for SARS-CoV-2 adds a highly adaptable tool to efforts to control the COVID-19 pandemic, and can inform test development strategies for future infectious disease threats." @default.
- W4214532250 created "2022-03-02" @default.
- W4214532250 creator A5004566101 @default.
- W4214532250 creator A5004659186 @default.
- W4214532250 creator A5006738835 @default.
- W4214532250 creator A5010663972 @default.
- W4214532250 creator A5034424299 @default.
- W4214532250 creator A5047834394 @default.
- W4214532250 creator A5064117402 @default.
- W4214532250 creator A5068353886 @default.
- W4214532250 creator A5072928802 @default.
- W4214532250 creator A5074433317 @default.
- W4214532250 creator A5076458101 @default.
- W4214532250 creator A5079236834 @default.
- W4214532250 creator A5085426603 @default.
- W4214532250 creator A5091885879 @default.
- W4214532250 date "2022-02-25" @default.
- W4214532250 modified "2023-09-26" @default.
- W4214532250 title "Sample-to-answer, extraction-free, real-time RT-LAMP test for SARS-CoV-2 in nasopharyngeal, nasal, and saliva samples: Implications and use for surveillance testing" @default.
- W4214532250 cites W158373933 @default.
- W4214532250 cites W1903563740 @default.
- W4214532250 cites W2070739465 @default.
- W4214532250 cites W2093274439 @default.
- W4214532250 cites W2141012160 @default.
- W4214532250 cites W2345450564 @default.
- W4214532250 cites W2589356716 @default.
- W4214532250 cites W2761449473 @default.
- W4214532250 cites W2800905921 @default.
- W4214532250 cites W2944434778 @default.
- W4214532250 cites W3011796477 @default.
- W4214532250 cites W3013893137 @default.
- W4214532250 cites W3014222358 @default.
- W4214532250 cites W3016250750 @default.
- W4214532250 cites W3016437870 @default.
- W4214532250 cites W3016615900 @default.
- W4214532250 cites W3017152347 @default.
- W4214532250 cites W3017228866 @default.
- W4214532250 cites W3017617595 @default.
- W4214532250 cites W3019218218 @default.
- W4214532250 cites W3025827838 @default.
- W4214532250 cites W3029210427 @default.
- W4214532250 cites W3031834099 @default.
- W4214532250 cites W3033546757 @default.
- W4214532250 cites W3034434235 @default.
- W4214532250 cites W3034747739 @default.
- W4214532250 cites W3037977837 @default.
- W4214532250 cites W3039624693 @default.
- W4214532250 cites W3040264860 @default.
- W4214532250 cites W3041024589 @default.
- W4214532250 cites W3041821205 @default.
- W4214532250 cites W3042813589 @default.
- W4214532250 cites W3045507769 @default.
- W4214532250 cites W3046859241 @default.
- W4214532250 cites W3048385964 @default.
- W4214532250 cites W3080652759 @default.
- W4214532250 cites W3083882437 @default.
- W4214532250 cites W3083982704 @default.
- W4214532250 cites W3085854736 @default.
- W4214532250 cites W3093690976 @default.
- W4214532250 cites W3095694526 @default.
- W4214532250 cites W3096640152 @default.
- W4214532250 cites W3098415323 @default.
- W4214532250 cites W3102037011 @default.
- W4214532250 cites W3108601158 @default.
- W4214532250 cites W3113716702 @default.
- W4214532250 cites W3119343341 @default.
- W4214532250 cites W3120226689 @default.
- W4214532250 cites W3121591075 @default.
- W4214532250 cites W3122815252 @default.
- W4214532250 cites W3122968958 @default.
- W4214532250 cites W3129858938 @default.
- W4214532250 cites W3131709588 @default.
- W4214532250 cites W3132630010 @default.
- W4214532250 cites W3133501852 @default.
- W4214532250 cites W3134567138 @default.
- W4214532250 cites W3135717820 @default.
- W4214532250 cites W3139092876 @default.
- W4214532250 cites W3151098594 @default.
- W4214532250 cites W3157367227 @default.
- W4214532250 cites W3158823402 @default.
- W4214532250 cites W3161714940 @default.
- W4214532250 cites W3180409609 @default.
- W4214532250 cites W3211539231 @default.
- W4214532250 cites W4206840134 @default.
- W4214532250 cites W4250000864 @default.
- W4214532250 cites W4295880516 @default.
- W4214532250 doi "https://doi.org/10.1371/journal.pone.0264130" @default.
- W4214532250 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35213596" @default.
- W4214532250 hasPublicationYear "2022" @default.
- W4214532250 type Work @default.
- W4214532250 citedByCount "16" @default.
- W4214532250 countsByYear W42145322502022 @default.
- W4214532250 countsByYear W42145322502023 @default.
- W4214532250 crossrefType "journal-article" @default.
- W4214532250 hasAuthorship W4214532250A5004566101 @default.
- W4214532250 hasAuthorship W4214532250A5004659186 @default.
- W4214532250 hasAuthorship W4214532250A5006738835 @default.
- W4214532250 hasAuthorship W4214532250A5010663972 @default.