FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4214532956> ?p ?o ?g. }

• W4214532956 endingPage "320" @default.
• W4214532956 startingPage "320" @default.
• W4214532956 abstract "Conventional cell patterning methods are mainly based on hydrophilic/hydrophobic differences or chemical coating for cell adhesion/non-adhesion with wavering strength as it varies with the substrate surface conditions, including the cell type and the extracellular matrix components (ECMs) coating; thus, the versatility and stability of cell patterning methods must be improved. In this study, we propose a new cell patterning method using a light-responsive gas-generating polymer (LGP) and a conventional fluorescence microscope. Herein, cells and cellular tissues are easily released from the substrate surface by the nitrogen gas bubbles generated from LGP by the excitation light for fluorescence observation without harming the cells. The LGP-implanted chip was fabricated by packing LGP into a polystyrene (PS) microarray chip with a concave pattern. HeLa cells were spread on the LGP-implanted chips coated with three different ECMs (fibronectin, collagen, and poly-D-lysine), and all HeLa cells on the three LGP patterns were released. The pattern error between the LGP pattern and the remaining HeLa cells was 8.81 ± 4.24 μm, less than single-cell size. In addition, the LGP-implanted chip method can be applied to millimeter-scale patterns, with less than 30 s required for cell patterning. Therefore, the proposed method is a simple and rapid cell patterning method with high cell patterning accuracy of less than the cell size error, high scalability, versatility, and stability unaffected by the cell type or the ECM coating." @default.
• W4214532956 created "2022-03-02" @default.
• W4214532956 creator A5000638727 @default.
• W4214532956 creator A5067228081 @default.
• W4214532956 creator A5087173459 @default.
• W4214532956 date "2022-02-18" @default.
• W4214532956 modified "2023-09-26" @default.
• W4214532956 title "Novel Quick Cell Patterning Using Light-Responsive Gas-Generating Polymer and Fluorescence Microscope" @default.
• W4214532956 cites W1973418324 @default.
• W4214532956 cites W1982897852 @default.
• W4214532956 cites W1985520972 @default.
• W4214532956 cites W1994186189 @default.
• W4214532956 cites W2009808362 @default.
• W4214532956 cites W2012152755 @default.
• W4214532956 cites W2018116089 @default.
• W4214532956 cites W2027704646 @default.
• W4214532956 cites W2034301798 @default.
• W4214532956 cites W2035729396 @default.
• W4214532956 cites W2057917964 @default.
• W4214532956 cites W2067361099 @default.
• W4214532956 cites W2080886982 @default.
• W4214532956 cites W2087967715 @default.
• W4214532956 cites W2111979255 @default.
• W4214532956 cites W2120454030 @default.
• W4214532956 cites W2319412230 @default.
• W4214532956 cites W2468862097 @default.
• W4214532956 cites W2505201296 @default.
• W4214532956 cites W2515248395 @default.
• W4214532956 cites W2563056436 @default.
• W4214532956 cites W2605115371 @default.
• W4214532956 cites W2765231276 @default.
• W4214532956 cites W2800843795 @default.
• W4214532956 cites W2895384939 @default.
• W4214532956 cites W2904887897 @default.
• W4214532956 cites W2951165982 @default.
• W4214532956 cites W3007510901 @default.
• W4214532956 cites W3011586769 @default.
• W4214532956 cites W3015790407 @default.
• W4214532956 cites W3025271557 @default.
• W4214532956 cites W3035510377 @default.
• W4214532956 cites W3037048284 @default.
• W4214532956 cites W3037310264 @default.
• W4214532956 cites W3047812799 @default.
• W4214532956 cites W3071416970 @default.
• W4214532956 cites W3113346353 @default.
• W4214532956 cites W3123938533 @default.
• W4214532956 doi "https://doi.org/10.3390/mi13020320" @default.
• W4214532956 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35208444" @default.
• W4214532956 hasPublicationYear "2022" @default.
• W4214532956 type Work @default.
• W4214532956 citedByCount "0" @default.
• W4214532956 crossrefType "journal-article" @default.
• W4214532956 hasAuthorship W4214532956A5000638727 @default.
• W4214532956 hasAuthorship W4214532956A5067228081 @default.
• W4214532956 hasAuthorship W4214532956A5087173459 @default.
• W4214532956 hasBestOaLocation W42145329561 @default.
• W4214532956 hasConcept C120665830 @default.
• W4214532956 hasConcept C121332964 @default.
• W4214532956 hasConcept C12554922 @default.
• W4214532956 hasConcept C136229726 @default.
• W4214532956 hasConcept C1491633281 @default.
• W4214532956 hasConcept C159985019 @default.
• W4214532956 hasConcept C169274487 @default.
• W4214532956 hasConcept C171250308 @default.
• W4214532956 hasConcept C185592680 @default.
• W4214532956 hasConcept C18903297 @default.
• W4214532956 hasConcept C189165786 @default.
• W4214532956 hasConcept C192562407 @default.
• W4214532956 hasConcept C2777289219 @default.
• W4214532956 hasConcept C2777366897 @default.
• W4214532956 hasConcept C2781448156 @default.
• W4214532956 hasConcept C521977710 @default.
• W4214532956 hasConcept C55493867 @default.
• W4214532956 hasConcept C71924100 @default.
• W4214532956 hasConcept C84416704 @default.
• W4214532956 hasConcept C85789140 @default.
• W4214532956 hasConcept C86492073 @default.
• W4214532956 hasConcept C86803240 @default.
• W4214532956 hasConcept C91881484 @default.
• W4214532956 hasConceptScore W4214532956C120665830 @default.
• W4214532956 hasConceptScore W4214532956C121332964 @default.
• W4214532956 hasConceptScore W4214532956C12554922 @default.
• W4214532956 hasConceptScore W4214532956C136229726 @default.
• W4214532956 hasConceptScore W4214532956C1491633281 @default.
• W4214532956 hasConceptScore W4214532956C159985019 @default.
• W4214532956 hasConceptScore W4214532956C169274487 @default.
• W4214532956 hasConceptScore W4214532956C171250308 @default.
• W4214532956 hasConceptScore W4214532956C185592680 @default.
• W4214532956 hasConceptScore W4214532956C18903297 @default.
• W4214532956 hasConceptScore W4214532956C189165786 @default.
• W4214532956 hasConceptScore W4214532956C192562407 @default.
• W4214532956 hasConceptScore W4214532956C2777289219 @default.
• W4214532956 hasConceptScore W4214532956C2777366897 @default.
• W4214532956 hasConceptScore W4214532956C2781448156 @default.
• W4214532956 hasConceptScore W4214532956C521977710 @default.
• W4214532956 hasConceptScore W4214532956C55493867 @default.
• W4214532956 hasConceptScore W4214532956C71924100 @default.
• W4214532956 hasConceptScore W4214532956C84416704 @default.

• next