FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4214656479> ?p ?o ?g. }

• W4214656479 endingPage "1086" @default.
• W4214656479 startingPage "1075" @default.
• W4214656479 abstract "We hypothesized that the general control nonderepressible 2 (GCN2)/eukaryotic initiation factor 2 (eIF2) signaling pathway and intracellular protein synthesis (PS) are regulated to maintain milk PS in primary bovine mammary epithelial cells (MECs) under essential amino acid (EAA) starvation conditions. We cultured MECs with 0%, 2% (depletion), and 100% (control) EAA for two exposure times (8 and 24 h), followed by three refeeding (RF) times with 100% EAA (0, 8, and 24 h). Subsequently, we measured cell viability, total protein concentration, and proliferation. Western blotting was used to quantify the levels of casein and the expression of total GCN2 and eIF2, as well as phosphorylated GCN2 (GCN2P) and eIF2 (eIF2P). The ISOQuant method was used to assess MEC proteomes, and the resultant data were analyzed using the Kruskal−Wallis test, nonpaired Wilcoxon rank post-hoc test, and ANOVA−Tukey test, as well as principal component analyses and multiple regressions models. Differences in cell viability were observed between the control versus the depleted and repleted MECs, respectively, where 97.2−99.8% viability indicated low cell death rates. Proliferation (range, 1.02−1.55 arbitrary units (AU)) was affected by starvation for 12 and 24 h and repletion for 24 h, but it was not increased compared with the control. Total protein expression was unaffected by both depletion and repletion treatments (median 3158 µg/mL). eIF2P expression was significantly increased (p < 0.05) after treatment with 2% EAA for 8 and 24 h compared with 2% EAA with 8 h + 24 h RF and 2% EAA with 24 h + 8 h RF. GCN2P also showed significantly increased expression (p < 0.05) after treatment with 2% EAA for 24 h compared with the control and 2% EAA with 24 h + 8 h RF. Intracellular casein/α-tubulin expression was unaffected by 2% EAA compared with control (0.073 ± 0.01 AU versus 0.086 ± 0.02 AU, respectively). We studied 30 of the detected 1180 proteins, 16 of which were differentially expressed in starved and refed MECs. Cells faced with EAA deficiency activated the GCN2P/eIF2P pathway, and the lack of change in the levels of casein and other milk proteins suggested that the EAA deficit was mitigated by metabolic flexibility to maintain homeostasis." @default.
• W4214656479 created "2022-03-02" @default.
• W4214656479 creator A5003954493 @default.
• W4214656479 creator A5029590004 @default.
• W4214656479 creator A5051885578 @default.
• W4214656479 date "2022-02-25" @default.
• W4214656479 modified "2023-10-18" @default.
• W4214656479 title "Effects of Essential Amino Acid Deficiency on General Control Nonderepressible 2/Eukaryotic Initiation Factor 2 Signaling and Proteomic Changes in Primary Bovine Mammary Epithelial Cells" @default.
• W4214656479 cites W1967891131 @default.
• W4214656479 cites W1985754783 @default.
• W4214656479 cites W1990909031 @default.
• W4214656479 cites W2001321742 @default.
• W4214656479 cites W2026724360 @default.
• W4214656479 cites W2046865491 @default.
• W4214656479 cites W2051638902 @default.
• W4214656479 cites W2051790918 @default.
• W4214656479 cites W2056036930 @default.
• W4214656479 cites W2059625648 @default.
• W4214656479 cites W2082422841 @default.
• W4214656479 cites W2085663116 @default.
• W4214656479 cites W2119554608 @default.
• W4214656479 cites W2123716335 @default.
• W4214656479 cites W2129260297 @default.
• W4214656479 cites W2134596328 @default.
• W4214656479 cites W2163639831 @default.
• W4214656479 cites W2168046351 @default.
• W4214656479 cites W2169845595 @default.
• W4214656479 cites W2299543412 @default.
• W4214656479 cites W2308569764 @default.
• W4214656479 cites W2327754435 @default.
• W4214656479 cites W2494956924 @default.
• W4214656479 cites W2526131915 @default.
• W4214656479 cites W2617696743 @default.
• W4214656479 cites W2757383676 @default.
• W4214656479 cites W2762986262 @default.
• W4214656479 cites W2794987006 @default.
• W4214656479 cites W2796260285 @default.
• W4214656479 cites W2906087943 @default.
• W4214656479 cites W2922006774 @default.
• W4214656479 cites W2966924465 @default.
• W4214656479 cites W3022681164 @default.
• W4214656479 cites W3095424764 @default.
• W4214656479 doi "https://doi.org/10.3390/cimb44030071" @default.
• W4214656479 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35723294" @default.
• W4214656479 hasPublicationYear "2022" @default.
• W4214656479 type Work @default.
• W4214656479 citedByCount "1" @default.
• W4214656479 crossrefType "journal-article" @default.
• W4214656479 hasAuthorship W4214656479A5003954493 @default.
• W4214656479 hasAuthorship W4214656479A5029590004 @default.
• W4214656479 hasAuthorship W4214656479A5051885578 @default.
• W4214656479 hasBestOaLocation W42146564791 @default.
• W4214656479 hasConcept C104317684 @default.
• W4214656479 hasConcept C105580179 @default.
• W4214656479 hasConcept C149364088 @default.
• W4214656479 hasConcept C153911025 @default.
• W4214656479 hasConcept C16685009 @default.
• W4214656479 hasConcept C185592680 @default.
• W4214656479 hasConcept C190283241 @default.
• W4214656479 hasConcept C53227056 @default.
• W4214656479 hasConcept C55493867 @default.
• W4214656479 hasConcept C62478195 @default.
• W4214656479 hasConcept C71924100 @default.
• W4214656479 hasConcept C79879829 @default.
• W4214656479 hasConcept C86803240 @default.
• W4214656479 hasConcept C93734116 @default.
• W4214656479 hasConcept C95444343 @default.
• W4214656479 hasConceptScore W4214656479C104317684 @default.
• W4214656479 hasConceptScore W4214656479C105580179 @default.
• W4214656479 hasConceptScore W4214656479C149364088 @default.
• W4214656479 hasConceptScore W4214656479C153911025 @default.
• W4214656479 hasConceptScore W4214656479C16685009 @default.
• W4214656479 hasConceptScore W4214656479C185592680 @default.
• W4214656479 hasConceptScore W4214656479C190283241 @default.
• W4214656479 hasConceptScore W4214656479C53227056 @default.
• W4214656479 hasConceptScore W4214656479C55493867 @default.
• W4214656479 hasConceptScore W4214656479C62478195 @default.
• W4214656479 hasConceptScore W4214656479C71924100 @default.
• W4214656479 hasConceptScore W4214656479C79879829 @default.
• W4214656479 hasConceptScore W4214656479C86803240 @default.
• W4214656479 hasConceptScore W4214656479C93734116 @default.
• W4214656479 hasConceptScore W4214656479C95444343 @default.
• W4214656479 hasIssue "3" @default.
• W4214656479 hasLocation W42146564791 @default.
• W4214656479 hasLocation W42146564792 @default.
• W4214656479 hasLocation W42146564793 @default.
• W4214656479 hasLocation W42146564794 @default.
• W4214656479 hasOpenAccess W4214656479 @default.
• W4214656479 hasPrimaryLocation W42146564791 @default.
• W4214656479 hasRelatedWork W1969886439 @default.
• W4214656479 hasRelatedWork W2091677039 @default.
• W4214656479 hasRelatedWork W2169979796 @default.
• W4214656479 hasRelatedWork W2347215050 @default.
• W4214656479 hasRelatedWork W2347357337 @default.
• W4214656479 hasRelatedWork W2347359022 @default.
• W4214656479 hasRelatedWork W2349779113 @default.
• W4214656479 hasRelatedWork W2363571182 @default.
• W4214656479 hasRelatedWork W2381004937 @default.

• next