Matches in SemOpenAlex for { <https://semopenalex.org/work/W4214658670> ?p ?o ?g. }
- W4214658670 endingPage "30" @default.
- W4214658670 startingPage "1" @default.
- W4214658670 abstract "Thermophiles, offering an attractive and unique platform for a broad range of applications in biofuels and environment protections, have received a significant attention and growing interest from academy and industry. However, the exploration and exploitation of thermophilic organisms have been hampered by the lack of a powerful genome manipulation tool to improve production efficiency. At current, the clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/CRISPR associated (Cas) system has been successfully exploited as a competent, simplistic, and powerful tool for genome engineering both in eukaryotes and prokaryotes. Indeed, with the significant efforts made in recent years, some thermostable Cas9 proteins have been well identified and characterized and further, some thermostable Cas9-based editing tools have been successfully established in some representative obligate thermophiles. In this regard, we reviewed the current status and its progress in CRISPR/Cas-based genome editing system towards a variety of thermophilic organisms. Despite the potentials of these progresses, multiple factors/barriers still have to be overcome and optimized for improving its editing efficiency in thermophiles. Some insights into the roles of thermostable CRISPR/Cas technologies for the metabolic engineering of thermophiles as a thermophilic microbial cell factory were also fully analyzed and discussed." @default.
- W4214658670 created "2022-03-02" @default.
- W4214658670 creator A5006381071 @default.
- W4214658670 creator A5017930564 @default.
- W4214658670 date "2022-01-01" @default.
- W4214658670 modified "2023-10-18" @default.
- W4214658670 title "CRISPR/Cas genome editing systems in thermophiles: Current status, associated challenges, and future perspectives" @default.
- W4214658670 cites W1501688989 @default.
- W4214658670 cites W1520537346 @default.
- W4214658670 cites W1529909008 @default.
- W4214658670 cites W1818937280 @default.
- W4214658670 cites W1970396053 @default.
- W4214658670 cites W1976290779 @default.
- W4214658670 cites W1980696141 @default.
- W4214658670 cites W1981563281 @default.
- W4214658670 cites W1983553894 @default.
- W4214658670 cites W1985703657 @default.
- W4214658670 cites W2000046950 @default.
- W4214658670 cites W2014869495 @default.
- W4214658670 cites W2018809073 @default.
- W4214658670 cites W2019376732 @default.
- W4214658670 cites W2019455696 @default.
- W4214658670 cites W2026822702 @default.
- W4214658670 cites W2028149625 @default.
- W4214658670 cites W2037162688 @default.
- W4214658670 cites W2045435533 @default.
- W4214658670 cites W2052135461 @default.
- W4214658670 cites W2054851997 @default.
- W4214658670 cites W2056958386 @default.
- W4214658670 cites W2063148079 @default.
- W4214658670 cites W2064462284 @default.
- W4214658670 cites W2073149731 @default.
- W4214658670 cites W2075305650 @default.
- W4214658670 cites W2078031016 @default.
- W4214658670 cites W2091864307 @default.
- W4214658670 cites W2092461073 @default.
- W4214658670 cites W2095745609 @default.
- W4214658670 cites W2099062342 @default.
- W4214658670 cites W2106450869 @default.
- W4214658670 cites W2107325876 @default.
- W4214658670 cites W2109517508 @default.
- W4214658670 cites W2111899354 @default.
- W4214658670 cites W2116711197 @default.
- W4214658670 cites W2119633529 @default.
- W4214658670 cites W2122540167 @default.
- W4214658670 cites W2122647535 @default.
- W4214658670 cites W2123746851 @default.
- W4214658670 cites W2126388436 @default.
- W4214658670 cites W2127885201 @default.
- W4214658670 cites W2132667417 @default.
- W4214658670 cites W2139935752 @default.
- W4214658670 cites W2143467231 @default.
- W4214658670 cites W2143918999 @default.
- W4214658670 cites W2143970553 @default.
- W4214658670 cites W2151301427 @default.
- W4214658670 cites W2153552126 @default.
- W4214658670 cites W2154149764 @default.
- W4214658670 cites W2156585782 @default.
- W4214658670 cites W2157288259 @default.
- W4214658670 cites W2158178789 @default.
- W4214658670 cites W2161457284 @default.
- W4214658670 cites W2165016943 @default.
- W4214658670 cites W2167177886 @default.
- W4214658670 cites W2167608581 @default.
- W4214658670 cites W2168393617 @default.
- W4214658670 cites W2169336338 @default.
- W4214658670 cites W2169744530 @default.
- W4214658670 cites W2259056198 @default.
- W4214658670 cites W2273690817 @default.
- W4214658670 cites W2282184745 @default.
- W4214658670 cites W2288204346 @default.
- W4214658670 cites W2298103687 @default.
- W4214658670 cites W2315022177 @default.
- W4214658670 cites W2337751047 @default.
- W4214658670 cites W2346158119 @default.
- W4214658670 cites W2366537989 @default.
- W4214658670 cites W2370021456 @default.
- W4214658670 cites W2472136907 @default.
- W4214658670 cites W2510496538 @default.
- W4214658670 cites W2510920746 @default.
- W4214658670 cites W2520569708 @default.
- W4214658670 cites W2528742798 @default.
- W4214658670 cites W2538295181 @default.
- W4214658670 cites W2545427125 @default.
- W4214658670 cites W2549981312 @default.
- W4214658670 cites W2557181747 @default.
- W4214658670 cites W2558343263 @default.
- W4214658670 cites W2583283187 @default.
- W4214658670 cites W2589046804 @default.
- W4214658670 cites W2592871788 @default.
- W4214658670 cites W2622258133 @default.
- W4214658670 cites W2623277795 @default.
- W4214658670 cites W2739917153 @default.
- W4214658670 cites W2755103163 @default.
- W4214658670 cites W2762091379 @default.
- W4214658670 cites W2769316169 @default.
- W4214658670 cites W2769919647 @default.
- W4214658670 cites W2770036411 @default.