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- W4214686475 abstract "Most plant and animal microRNAs (miRNAs) are transcribed by RNA polymerase II. We previously discovered miRNA-like small RNAs (milRNAs) in the filamentous fungus Neurospora crassa and uncovered at least four different pathways for milRNA production. To understand the evolutionary origin of milRNAs, we determined the roles of polymerases II and III (Pol II and Pol III) in milRNA transcription. Our results show that Pol III is responsible for the transcription of the major milRNAs produced in this organism. The inhibition of Pol III activity by an inhibitor or by gene silencing abolishes the production of most abundant milRNAs and pri-milRNAs. In addition, Pol III associates with these milRNA producing loci. Even though silencing of Pol II does not affect the synthesis of the most abundant milRNAs, Pol II or both Pol II and Pol III are associated with some milRNA-producing loci, suggesting a regulatory interaction between the two polymerases for some milRNA transcription. Furthermore, we show that one of the Pol III-transcribed milRNAs is derived from a tRNA precursor, and its biogenesis requires RNase Z, which cleaves the tRNA moiety to generate pre-milRNA. Our study identifies the transcriptional machinery responsible for the synthesis of fungal milRNAs and sheds light on the evolutionary origin of eukaryotic small RNAs. PMID: 23349642 Funding information This work was supported by: NIGMS NIH HHS, United States Grant ID: GM068496 NIGMS NIH HHS, United States Grant ID: GM062591 NIGMS NIH HHS, United States Grant ID: R35 GM118118 NIGMS NIH HHS, United States Grant ID: R01 GM084283" @default.
- W4214686475 created "2022-03-02" @default.
- W4214686475 creator A5052869398 @default.
- W4214686475 date "2013-02-02" @default.
- W4214686475 modified "2023-09-28" @default.
- W4214686475 title "Faculty Opinions recommendation of Transcription of the major neurospora crassa microRNA-like small RNAs relies on RNA polymerase III." @default.
- W4214686475 doi "https://doi.org/10.3410/f.717974905.793470291" @default.
- W4214686475 hasPublicationYear "2013" @default.
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