Matches in SemOpenAlex for { <https://semopenalex.org/work/W4214714747> ?p ?o ?g. }
- W4214714747 abstract "Abstract Biotechnological production processes are sustainable approaches for the production of biobased components such as amino acids for food and feed industry. Scale‐up from ideal lab‐scale bioreactors to large‐scale processes is often accompanied by loss in productivity. This may be related to population heterogeneities of cells originating from isogenic cultures that arise due to dynamic non‐ideal conditions in the bioreactor. To better understand this phenomenon, deeper insights into single‐cell physiologies in bioprocesses are mandatory before scale‐up. Here, a triple reporter strain (3RP) was developed by chromosomally integrating the fluorescent proteins mEmerald, CyOFP1, and mTagBFP2 into the L‐phenylalanine producing Escherichia coli strain FUS4 (pF81 kan ) to allow monitoring of growth, oxygen availability, and general stress response of the single cells. Functionality of the 3RP was confirmed in well‐mixed lab‐scale fed‐batch processes with glycerol as carbon source in comparison to the strain without fluorescent proteins, leading to no difference in process performance. Fluorescence levels could successfully reflect the course of related process state variables, revealed population heterogeneities during the transition between different process phases and potentially subpopulations that exhibit superior process performance. Furthermore, indications were found for noise in gene expression as regulation strategy against environmental perturbation." @default.
- W4214714747 created "2022-03-02" @default.
- W4214714747 creator A5001116587 @default.
- W4214714747 creator A5006478727 @default.
- W4214714747 creator A5013666983 @default.
- W4214714747 creator A5015932606 @default.
- W4214714747 creator A5037562484 @default.
- W4214714747 creator A5040121825 @default.
- W4214714747 date "2022-02-26" @default.
- W4214714747 modified "2023-10-14" @default.
- W4214714747 title "Application of an <i>Escherichia coli</i> triple reporter strain for <i>at‐line</i> monitoring of single‐cell physiology during L‐phenylalanine production" @default.
- W4214714747 cites W126841054 @default.
- W4214714747 cites W1580639168 @default.
- W4214714747 cites W1645305843 @default.
- W4214714747 cites W1967663232 @default.
- W4214714747 cites W1970426717 @default.
- W4214714747 cites W1972829330 @default.
- W4214714747 cites W1975838895 @default.
- W4214714747 cites W1980740090 @default.
- W4214714747 cites W1981457705 @default.
- W4214714747 cites W1987573660 @default.
- W4214714747 cites W2013846423 @default.
- W4214714747 cites W2015199121 @default.
- W4214714747 cites W2016786372 @default.
- W4214714747 cites W2041786205 @default.
- W4214714747 cites W2056228650 @default.
- W4214714747 cites W2058211348 @default.
- W4214714747 cites W2063792424 @default.
- W4214714747 cites W2073808637 @default.
- W4214714747 cites W2073966355 @default.
- W4214714747 cites W2074692830 @default.
- W4214714747 cites W2075353846 @default.
- W4214714747 cites W2080500319 @default.
- W4214714747 cites W2083599438 @default.
- W4214714747 cites W2085796061 @default.
- W4214714747 cites W2097045267 @default.
- W4214714747 cites W2097464440 @default.
- W4214714747 cites W2103072970 @default.
- W4214714747 cites W2109171263 @default.
- W4214714747 cites W2109242001 @default.
- W4214714747 cites W2113033658 @default.
- W4214714747 cites W2113476954 @default.
- W4214714747 cites W2113487205 @default.
- W4214714747 cites W2117597548 @default.
- W4214714747 cites W2118829612 @default.
- W4214714747 cites W2118898625 @default.
- W4214714747 cites W2132990040 @default.
- W4214714747 cites W2133091545 @default.
- W4214714747 cites W2137018402 @default.
- W4214714747 cites W2143094150 @default.
- W4214714747 cites W2146538795 @default.
- W4214714747 cites W2148325536 @default.
- W4214714747 cites W2150392611 @default.
- W4214714747 cites W2150755509 @default.
- W4214714747 cites W2152428375 @default.
- W4214714747 cites W2164734819 @default.
- W4214714747 cites W2165202736 @default.
- W4214714747 cites W2171529481 @default.
- W4214714747 cites W2180588273 @default.
- W4214714747 cites W2187281001 @default.
- W4214714747 cites W2263031789 @default.
- W4214714747 cites W2288937432 @default.
- W4214714747 cites W2298917721 @default.
- W4214714747 cites W2415046030 @default.
- W4214714747 cites W2548873975 @default.
- W4214714747 cites W2586378157 @default.
- W4214714747 cites W2605136394 @default.
- W4214714747 cites W2608758311 @default.
- W4214714747 cites W2723535088 @default.
- W4214714747 cites W2768425954 @default.
- W4214714747 cites W2793282492 @default.
- W4214714747 cites W2796836297 @default.
- W4214714747 cites W2801311059 @default.
- W4214714747 cites W2807058453 @default.
- W4214714747 cites W2950677425 @default.
- W4214714747 cites W2950849909 @default.
- W4214714747 cites W2959442962 @default.
- W4214714747 cites W3003565000 @default.
- W4214714747 cites W3212461664 @default.
- W4214714747 doi "https://doi.org/10.1002/elsc.202100162" @default.
- W4214714747 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36619877" @default.
- W4214714747 hasPublicationYear "2022" @default.
- W4214714747 type Work @default.
- W4214714747 citedByCount "3" @default.
- W4214714747 countsByYear W42147147472022 @default.
- W4214714747 countsByYear W42147147472023 @default.
- W4214714747 crossrefType "journal-article" @default.
- W4214714747 hasAuthorship W4214714747A5001116587 @default.
- W4214714747 hasAuthorship W4214714747A5006478727 @default.
- W4214714747 hasAuthorship W4214714747A5013666983 @default.
- W4214714747 hasAuthorship W4214714747A5015932606 @default.
- W4214714747 hasAuthorship W4214714747A5037562484 @default.
- W4214714747 hasAuthorship W4214714747A5040121825 @default.
- W4214714747 hasBestOaLocation W42147147471 @default.
- W4214714747 hasConcept C104317684 @default.
- W4214714747 hasConcept C105702510 @default.
- W4214714747 hasConcept C127413603 @default.
- W4214714747 hasConcept C142613039 @default.
- W4214714747 hasConcept C144024400 @default.
- W4214714747 hasConcept C149923435 @default.