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- W4214758480 abstract "After obtaining the raw measurements as gene copies per litre using RT-qPCR, a normalisation process is required prior to reporting the data as RNA copies per person. This is because the concentration of viral RNA in wastewater is affected by both the population of the catchment area at each waterworks, as well as the amount of flow into the works. For example, an area with heavy rainfall will have high volumes of fluid flow, which will dilute RNA values. Therefore, parameters such as the flow volume of wastewater and population size are used to overcome this bias. Unfortunately, the flow data are not always accessible for all sites. Three methods were developed by Biomathematics and Statistics Scotland (BioSS) to estimate the flow for the normalization process, depending on data availability: 1. Normalisation using ammonia concentration to estimate flow; 2. Normalisation using flow historical average (when data for method 1 are not available); 3. Normalisation using predicted ammonia to estimate flow (when data for methods 1 and 2 are not available). For all methods, the normalised outputs were reported as a daily value of RNA copies per person. These methods are described separately in this protocol. For other methodologies involving SARS-CoV-2 quantification in wastewater, such as viral RNA isolation and RT-qPCR, please refer to: dx.doi.org/10.17504/protocols.io.bzv5p686 (RNA extraction from wastewater for detection of SARS-CoV-2) dx.doi.org/10.17504/protocols.io.bzwap7ae (RT-qPCR for detection of SARS-CoV-2 in wastewater)" @default.
- W4214758480 created "2022-03-02" @default.
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- W4214758480 date "2022-01-27" @default.
- W4214758480 modified "2023-09-28" @default.
- W4214758480 title "Data normalisation of RT-qPCR data for detection of SARS-CoV-2 in wastewater v1" @default.
- W4214758480 doi "https://doi.org/10.17504/protocols.io.b4eqqtdw" @default.
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