FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4214842968> ?p ?o ?g. }

• W4214842968 abstract "Abstract Apicomplexan parasites like Toxoplasma gondii grow and replicate within a specialized organelle called the parasitophorous vacuole. The vacuole is decorated with parasite proteins that integrate into the membrane after trafficking through the parasite secretory system as soluble, chaperoned complexes. A regulator of this process is an atypical protein kinase called WNG1. Phosphorylation by WNG1 appears to serve as a switch for membrane integration. However, like its substrates, WNG1 is secreted from the parasite dense granules, and its activity must therefore be tightly regulated until the correct membrane is encountered. Here we demonstrate that, while another member of the WNG family can adopt multiple multimeric states, WNG1 is monomeric and therefore not regulated by multimerization. Instead, we identify two phosphosites on WNG1 that are required for its kinase activity. Using a combination of in vitro biochemistry and structural modeling, we identify basic residues that are also required for WNG1 activity and therefore appear to recognize the activating phosphosites. Among these coordinating residues are the “HRD” Arg, which recognizes activation loop phosphorylation in canonical kinases. WNG1, however, is not phosphorylated on its activation loop, and its activating phosphosites instead appear to lock the kinase C-lobe into an activated conformation. These data suggest a simple model for WNG1 activation by increasing ATP concentration above a critical threshold once WNG1 traffics to the parasitophorous vacuole." @default.
• W4214842968 created "2022-03-05" @default.
• W4214842968 creator A5010653678 @default.
• W4214842968 creator A5011608203 @default.
• W4214842968 creator A5017968230 @default.
• W4214842968 creator A5019601396 @default.
• W4214842968 creator A5048249606 @default.
• W4214842968 creator A5062731445 @default.
• W4214842968 date "2022-03-02" @default.
• W4214842968 modified "2023-09-27" @default.
• W4214842968 title "Divergent kinase WNG1 is regulated by phosphorylation of an atypical activation sub-domain" @default.
• W4214842968 cites W124504601 @default.
• W4214842968 cites W1532900970 @default.
• W4214842968 cites W1952642745 @default.
• W4214842968 cites W1964504843 @default.
• W4214842968 cites W1964641696 @default.
• W4214842968 cites W1972426139 @default.
• W4214842968 cites W1977472414 @default.
• W4214842968 cites W1978329718 @default.
• W4214842968 cites W1987428363 @default.
• W4214842968 cites W1993504559 @default.
• W4214842968 cites W1996372785 @default.
• W4214842968 cites W2008566951 @default.
• W4214842968 cites W2021105093 @default.
• W4214842968 cites W2021375991 @default.
• W4214842968 cites W2028293593 @default.
• W4214842968 cites W2030205108 @default.
• W4214842968 cites W2042916215 @default.
• W4214842968 cites W2046598192 @default.
• W4214842968 cites W2049145190 @default.
• W4214842968 cites W2056017147 @default.
• W4214842968 cites W2057212626 @default.
• W4214842968 cites W2063877414 @default.
• W4214842968 cites W2065283382 @default.
• W4214842968 cites W2066606532 @default.
• W4214842968 cites W2072674961 @default.
• W4214842968 cites W2089686093 @default.
• W4214842968 cites W2090763498 @default.
• W4214842968 cites W2094804194 @default.
• W4214842968 cites W2102025585 @default.
• W4214842968 cites W2104485296 @default.
• W4214842968 cites W2108342610 @default.
• W4214842968 cites W2114241062 @default.
• W4214842968 cites W2115969107 @default.
• W4214842968 cites W2117355601 @default.
• W4214842968 cites W2128705810 @default.
• W4214842968 cites W2135394966 @default.
• W4214842968 cites W2140490890 @default.
• W4214842968 cites W2140992384 @default.
• W4214842968 cites W2142337868 @default.
• W4214842968 cites W2154706050 @default.
• W4214842968 cites W2163188200 @default.
• W4214842968 cites W2169364535 @default.
• W4214842968 cites W2173371691 @default.
• W4214842968 cites W2274389043 @default.
• W4214842968 cites W2400773437 @default.
• W4214842968 cites W2921753065 @default.
• W4214842968 cites W3109413273 @default.
• W4214842968 doi "https://doi.org/10.1101/2022.03.02.482640" @default.
• W4214842968 hasPublicationYear "2022" @default.
• W4214842968 type Work @default.
• W4214842968 citedByCount "0" @default.
• W4214842968 crossrefType "posted-content" @default.
• W4214842968 hasAuthorship W4214842968A5010653678 @default.
• W4214842968 hasAuthorship W4214842968A5011608203 @default.
• W4214842968 hasAuthorship W4214842968A5017968230 @default.
• W4214842968 hasAuthorship W4214842968A5019601396 @default.
• W4214842968 hasAuthorship W4214842968A5048249606 @default.
• W4214842968 hasAuthorship W4214842968A5062731445 @default.
• W4214842968 hasBestOaLocation W42148429681 @default.
• W4214842968 hasConcept C102568950 @default.
• W4214842968 hasConcept C104317684 @default.
• W4214842968 hasConcept C11960822 @default.
• W4214842968 hasConcept C143065580 @default.
• W4214842968 hasConcept C168240541 @default.
• W4214842968 hasConcept C184235292 @default.
• W4214842968 hasConcept C190062978 @default.
• W4214842968 hasConcept C195794163 @default.
• W4214842968 hasConcept C32619005 @default.
• W4214842968 hasConcept C55493867 @default.
• W4214842968 hasConcept C86803240 @default.
• W4214842968 hasConcept C95444343 @default.
• W4214842968 hasConcept C97029542 @default.
• W4214842968 hasConceptScore W4214842968C102568950 @default.
• W4214842968 hasConceptScore W4214842968C104317684 @default.
• W4214842968 hasConceptScore W4214842968C11960822 @default.
• W4214842968 hasConceptScore W4214842968C143065580 @default.
• W4214842968 hasConceptScore W4214842968C168240541 @default.
• W4214842968 hasConceptScore W4214842968C184235292 @default.
• W4214842968 hasConceptScore W4214842968C190062978 @default.
• W4214842968 hasConceptScore W4214842968C195794163 @default.
• W4214842968 hasConceptScore W4214842968C32619005 @default.
• W4214842968 hasConceptScore W4214842968C55493867 @default.
• W4214842968 hasConceptScore W4214842968C86803240 @default.
• W4214842968 hasConceptScore W4214842968C95444343 @default.
• W4214842968 hasConceptScore W4214842968C97029542 @default.
• W4214842968 hasLocation W42148429681 @default.
• W4214842968 hasLocation W42148429682 @default.
• W4214842968 hasOpenAccess W4214842968 @default.
• W4214842968 hasPrimaryLocation W42148429681 @default.

• next