Matches in SemOpenAlex for { <https://semopenalex.org/work/W4214854235> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W4214854235 endingPage "23" @default.
- W4214854235 startingPage "16" @default.
- W4214854235 abstract "The capability to detect Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection and identify immune responses among the population is crucial for managing the outbreak of the COVID-19 pandemic. Although PCR-based nucleic acid detection techniques are utilized to detect viral infection in people, alternative tests capable of distinguishing between exposure and infection are urgently needed beyond this restricted window of detectable viral replication. Antibodies are produced in human sera within a few days after viral infection, providing longer period for performing tests to acquire reliable database. Herewith, we provide the results of our in-house developed ELISA (Enzyme-Linked Immunosorbent Assay) that displays all of the properties necessary for high-throughput of human sera sample analysis. This test does not involve the handling of live viruses, although it detects a variety of antibody types in serum and plasma of human after exposure to the virus. For in-house development of the kit, the nucleocapsid (N) gene of SARS-CoV-2 virus was cloned in the prokaryotic expression vector pGEX-6P-1, and purified N protein was used to detect IgG antibodies in human sera samples. In total 76 human serum samples that were collected before novel coronavirus registry in Mongolia in March 2020, as well as 200 serum samples from patients who had been infected by SARS-CoV-2 virus, were used. Among 200 serum samples, 188 were positive and 12 were false negative, while in non-infected cases 69 were negative and 7 were false positive, suggesting 94 per cent sensitivity and 90.7 per cent specificity of the kit, with p-values of 0.02." @default.
- W4214854235 created "2022-03-05" @default.
- W4214854235 creator A5000266912 @default.
- W4214854235 creator A5001159302 @default.
- W4214854235 creator A5002084345 @default.
- W4214854235 creator A5004836490 @default.
- W4214854235 creator A5005168657 @default.
- W4214854235 creator A5024538287 @default.
- W4214854235 creator A5038775263 @default.
- W4214854235 creator A5039722831 @default.
- W4214854235 creator A5045039869 @default.
- W4214854235 creator A5046231952 @default.
- W4214854235 creator A5048258556 @default.
- W4214854235 creator A5082544957 @default.
- W4214854235 date "2021-09-29" @default.
- W4214854235 modified "2023-09-27" @default.
- W4214854235 title "Development of serological assay for detection of antibodies in the N protein of SARS-CoV-2 in human sera in Mongolia" @default.
- W4214854235 doi "https://doi.org/10.5564/pmas.v61i03.1819" @default.
- W4214854235 hasPublicationYear "2021" @default.
- W4214854235 type Work @default.
- W4214854235 citedByCount "0" @default.
- W4214854235 crossrefType "journal-article" @default.
- W4214854235 hasAuthorship W4214854235A5000266912 @default.
- W4214854235 hasAuthorship W4214854235A5001159302 @default.
- W4214854235 hasAuthorship W4214854235A5002084345 @default.
- W4214854235 hasAuthorship W4214854235A5004836490 @default.
- W4214854235 hasAuthorship W4214854235A5005168657 @default.
- W4214854235 hasAuthorship W4214854235A5024538287 @default.
- W4214854235 hasAuthorship W4214854235A5038775263 @default.
- W4214854235 hasAuthorship W4214854235A5039722831 @default.
- W4214854235 hasAuthorship W4214854235A5045039869 @default.
- W4214854235 hasAuthorship W4214854235A5046231952 @default.
- W4214854235 hasAuthorship W4214854235A5048258556 @default.
- W4214854235 hasAuthorship W4214854235A5082544957 @default.
- W4214854235 hasBestOaLocation W42148542351 @default.
- W4214854235 hasConcept C116675565 @default.
- W4214854235 hasConcept C159047783 @default.
- W4214854235 hasConcept C159654299 @default.
- W4214854235 hasConcept C203014093 @default.
- W4214854235 hasConcept C2522874641 @default.
- W4214854235 hasConcept C2908647359 @default.
- W4214854235 hasConcept C45189115 @default.
- W4214854235 hasConcept C71924100 @default.
- W4214854235 hasConcept C86803240 @default.
- W4214854235 hasConcept C99454951 @default.
- W4214854235 hasConceptScore W4214854235C116675565 @default.
- W4214854235 hasConceptScore W4214854235C159047783 @default.
- W4214854235 hasConceptScore W4214854235C159654299 @default.
- W4214854235 hasConceptScore W4214854235C203014093 @default.
- W4214854235 hasConceptScore W4214854235C2522874641 @default.
- W4214854235 hasConceptScore W4214854235C2908647359 @default.
- W4214854235 hasConceptScore W4214854235C45189115 @default.
- W4214854235 hasConceptScore W4214854235C71924100 @default.
- W4214854235 hasConceptScore W4214854235C86803240 @default.
- W4214854235 hasConceptScore W4214854235C99454951 @default.
- W4214854235 hasLocation W42148542351 @default.
- W4214854235 hasOpenAccess W4214854235 @default.
- W4214854235 hasPrimaryLocation W42148542351 @default.
- W4214854235 hasRelatedWork W110646739 @default.
- W4214854235 hasRelatedWork W1487857142 @default.
- W4214854235 hasRelatedWork W1538985698 @default.
- W4214854235 hasRelatedWork W2075503308 @default.
- W4214854235 hasRelatedWork W2085137006 @default.
- W4214854235 hasRelatedWork W2139983200 @default.
- W4214854235 hasRelatedWork W2141071617 @default.
- W4214854235 hasRelatedWork W2435535700 @default.
- W4214854235 hasRelatedWork W293776161 @default.
- W4214854235 hasRelatedWork W4290202528 @default.
- W4214854235 isParatext "false" @default.
- W4214854235 isRetracted "false" @default.
- W4214854235 workType "article" @default.