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- W4214863676 abstract "A survey of our recent efforts to develop fiber-optic biocatalytic biosensors is presented. Examples of fiber-optic biosensors based on absorbance, fluorescence, and bioluminescence phenomena are described. Absorbance-based biosensors are demonstrated with a sensor for p-nitrophenylphosphate. Alkaline phosphatase is immobilized at the tip of a bifurcated fiber-optic bundle and the biocatalytic generation of the chromophore p-nitrophenoxide is monitored. Fluorescence-based biosensors for lactate and pyruvate are used to illustrate the principles of biosensors based on the fluorometric detection of produced or consumed reduced nicotinamide adenine dinucleotide (NADH). The concept of biosensors based on a bioluminescence process is demonstrated with sensors for NADH and glutamate. In addition, a novel internal enzyme biosensor arrangement is described where the active enzyme is separated from the sample by a perm-selective membrane. An internal enzyme biosensor for ethanol is presented and the relative merits of this novel biosensor design are discussed. Finally, biosensors based on the combination of a deaminating enzyme with a fiber-optic ammonia gassensing probe are demonstrated with a biosensor for glutamate. Here, glutamate oxidase is the immobilized enzyme and the production of ammonia is measured fluorometrically." @default.
- W4214863676 created "2022-03-05" @default.
- W4214863676 creator A5085986987 @default.
- W4214863676 date "1989-08-24" @default.
- W4214863676 modified "2023-09-26" @default.
- W4214863676 title "Fiber-Optic-Based Biocatalytic Biosensors" @default.
- W4214863676 doi "https://doi.org/10.1021/bk-1989-0403.ch020" @default.
- W4214863676 hasPublicationYear "1989" @default.
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