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• W4214893708 abstract "Abstract Foodborne illness outbreaks caused by consuming cantaloupe melon contaminated with Salmonella spp. and Escherichia coli O157:H7 are recurrent. The objective of this work was to investigate the antimicrobial effect of the combination of chitosan (Q) and extracellular metabolites of Pediococcus pentosaceus CM175 (Em Pp ) on the surface of cantaloupe against E. coli O157:H7 and Salmonella Typhimurium. The minimum inhibitory and bactericide concentrations (MIC and MBC) of Q and Em Pp were determined by the method of microdilution in broth and viability in plate, respectively. The antimicrobial effect derived from the combination of Q and Em Pp was evaluated using the chessboard technique. The antimicrobial activity of the different treatments in cantaloupe was performed employing plate counting. The viability and membrane integrity of S . Typhimurium and E. coli O157:H7 after treatments application were observed by fluorescence microscopy. The results showed that MIC and MBC of Q against S . Typhimurium were 0.1% and 0.7%, respectively; 0.004% and 0.03% for E. coli O157:H7, respectively; for Em Pp , MIC was 8.0% and MBC was 10% for both bacteria. The combination of 0.1% Q and 6.6% Em Pp against S . Typhimurium and 0.01% Q and 5.0% Em Pp against E. coli O157:H7 showed an additive effect. The highest reductions of S . Typhimurium and E. coli O157:H7 in cantaloupe were exerted by the combinations of 0.1% Q with 6.6% Em Pp and 0.01% Q with 5.0% Em Pp , respectively. These results show that combination of Q and Em Pp could be an effective and promising alternative against the tested pathogens." @default.
• W4214893708 created "2022-03-05" @default.
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• W4214893708 date "2022-03-02" @default.
• W4214893708 modified "2023-10-17" @default.
• W4214893708 title "Antimicrobial effect of chitosan and extracellular metabolites of <i>Pediococcus pentosaceus</i> CM175 against <i>Salmonella</i> Typhimurium and <i>Escherichia coli</i> O157:H7" @default.
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• W4214893708 doi "https://doi.org/10.1111/jfs.12968" @default.
• W4214893708 hasPublicationYear "2022" @default.
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