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• W4214906937 abstract "Objective Increased apoptotic shedding has been linked to intestinal barrier dysfunction and development of inflammatory bowel diseases (IBD). In contrast, physiological cell shedding allows the renewal of the epithelial monolayer without compromising the barrier function. Here, we investigated the role of live cell extrusion in epithelial barrier alterations in IBD. Design Taking advantage of conditional GGTase and RAC1 knockout mice in intestinal epithelial cells ( Pggt1b iΔIEC and Rac1 iΔIEC mice), intravital microscopy, immunostaining, mechanobiology, organoid techniques and RNA sequencing, we analysed cell shedding alterations within the intestinal epithelium. Moreover, we examined human gut tissue and intestinal organoids from patients with IBD for cell shedding alterations and RAC1 function. Results Epithelial Pggt1b deletion led to cytoskeleton rearrangement and tight junction redistribution, causing cell overcrowding due to arresting of cell shedding that finally resulted in epithelial leakage and spontaneous mucosal inflammation in the small and to a lesser extent in the large intestine. Both in vivo and in vitro studies (knockout mice, organoids) identified RAC1 as a GGTase target critically involved in prenylation-dependent cytoskeleton dynamics, cell mechanics and epithelial cell shedding. Moreover, inflamed areas of gut tissue from patients with IBD exhibited funnel-like structures, signs of arrested cell shedding and impaired RAC1 function. RAC1 inhibition in human intestinal organoids caused actin alterations compatible with arresting of cell shedding. Conclusion Impaired epithelial RAC1 function causes cell overcrowding and epithelial leakage thus inducing chronic intestinal inflammation. Epithelial RAC1 emerges as key regulator of cytoskeletal dynamics, cell mechanics and intestinal cell shedding. Modulation of RAC1 might be exploited for restoration of epithelial integrity in the gut of patients with IBD." @default.
• W4214906937 created "2022-03-05" @default.
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• W4214906937 date "2022-03-03" @default.
• W4214906937 modified "2023-10-18" @default.
• W4214906937 title "Epithelial RAC1-dependent cytoskeleton dynamics controls cell mechanics, cell shedding and barrier integrity in intestinal inflammation" @default.
• W4214906937 cites W1864325817 @default.
• W4214906937 cites W1975523864 @default.
• W4214906937 cites W1980147115 @default.
• W4214906937 cites W1986047072 @default.
• W4214906937 cites W1990123556 @default.
• W4214906937 cites W1991346408 @default.
• W4214906937 cites W1993184653 @default.
• W4214906937 cites W2005758722 @default.
• W4214906937 cites W2018631917 @default.
• W4214906937 cites W2023072510 @default.
• W4214906937 cites W2025065287 @default.
• W4214906937 cites W2032380005 @default.
• W4214906937 cites W2035807711 @default.
• W4214906937 cites W2036164824 @default.
• W4214906937 cites W2039632779 @default.
• W4214906937 cites W2050669247 @default.
• W4214906937 cites W2051016124 @default.
• W4214906937 cites W2055847901 @default.
• W4214906937 cites W2061791452 @default.
• W4214906937 cites W2081951192 @default.
• W4214906937 cites W2083671963 @default.
• W4214906937 cites W2084423192 @default.
• W4214906937 cites W2084961029 @default.
• W4214906937 cites W2086681622 @default.
• W4214906937 cites W2092634655 @default.
• W4214906937 cites W2100093202 @default.
• W4214906937 cites W2103569939 @default.
• W4214906937 cites W2106145925 @default.
• W4214906937 cites W2106874535 @default.
• W4214906937 cites W2108963851 @default.
• W4214906937 cites W2112129392 @default.
• W4214906937 cites W2117427879 @default.
• W4214906937 cites W2121707980 @default.
• W4214906937 cites W2122249218 @default.
• W4214906937 cites W2125808023 @default.
• W4214906937 cites W2134657042 @default.
• W4214906937 cites W2136628679 @default.
• W4214906937 cites W2141710693 @default.
• W4214906937 cites W2145189896 @default.
• W4214906937 cites W2146367607 @default.
• W4214906937 cites W2149899704 @default.
• W4214906937 cites W2153210191 @default.
• W4214906937 cites W2157408682 @default.
• W4214906937 cites W2159695799 @default.
• W4214906937 cites W2159861680 @default.
• W4214906937 cites W2166710590 @default.
• W4214906937 cites W2167062644 @default.
• W4214906937 cites W2170733892 @default.
• W4214906937 cites W2234676809 @default.
• W4214906937 cites W2319760762 @default.
• W4214906937 cites W2340195463 @default.
• W4214906937 cites W2343478579 @default.
• W4214906937 cites W2601759044 @default.
• W4214906937 cites W2603828250 @default.
• W4214906937 cites W2619114066 @default.
• W4214906937 cites W2646476739 @default.
• W4214906937 cites W2778004748 @default.
• W4214906937 cites W2796144999 @default.
• W4214906937 cites W2801858122 @default.
• W4214906937 cites W2890618112 @default.
• W4214906937 cites W2933961522 @default.
• W4214906937 cites W2958375893 @default.
• W4214906937 cites W2961328304 @default.
• W4214906937 cites W2963978284 @default.
• W4214906937 cites W2967076383 @default.

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