FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4214938378> ?p ?o ?g. }

• W4214938378 endingPage "944" @default.
• W4214938378 startingPage "938" @default.
• W4214938378 abstract "Abstract Metagenetic methods are commonplace within ecological and environmental research. One concern with these methods is the phenomenon of ‘critical mistagging’, where sequences from one sample are erroneously inferred to have originated from another sample due to errors in the attachment, PCR replication or sequencing of sample‐specific dual‐index tags. For studies using PCR‐based library preparation on large sample sizes, the most cost‐effective approach to limiting mistag‐associated false detections involves using an unsaturated Latin square dual‐indexing design. This allows researchers to estimate mistagging rates during sequencing but the statistical procedures for filtering out detections using this mistag rate have received little attention. I propose a straightforward method to limit mistag‐associated false discoveries during metabarcoding applications. I analysed two Illumina metabarcoding datasets produced using unsaturated Latin square designs to explore the distribution of mistagged sequences across dual‐index combinations on a per taxon basis. I tested these data for conformity to the assumptions that (a) mistagging follows a binomial distribution (i.e. X ~ B ( n , p )) where p , the probability of a sequence being mistagged, varies minimally across taxa and (b) mistags are distributed uniformly across dual‐index combinations. I provide R functions that estimate the 95th percentile of expected mistags per dual‐index combination for each taxon under these assumptions. I show that mistagging rates were consistent across taxa within the datasets analysed and that modelling mistagging as a binomial process with uniform distribution across dual‐index combinations enabled robust control of mistag‐associated false discoveries. I propose that this method of taxon‐specific filtering of detections based on the maximum mistags expected per dual‐index combination should be broadly accepted during metagenetic analysis, provided that control sample mistag abundances follow a near‐uniform distribution. When this assumption is violated, data may be better fit by assuming that the distribution of mistags across combinations follows Poisson characteristics (i.e. X ~Pois(λ)), with λ empirically estimated from the abundance distribution of mistags among control samples. I provide a second R function for this case. Both functions and demonstrations associated with this work are freely available at https://github.com/RTRichar/ModellingCriticalMistags ." @default.
• W4214938378 created "2022-03-05" @default.
• W4214938378 creator A5071744559 @default.
• W4214938378 date "2022-03-29" @default.
• W4214938378 modified "2023-10-09" @default.
• W4214938378 title "Controlling critical mistag‐associated false discoveries in metagenetic data" @default.
• W4214938378 cites W1977899163 @default.
• W4214938378 cites W2060329218 @default.
• W4214938378 cites W2061211738 @default.
• W4214938378 cites W2128711701 @default.
• W4214938378 cites W2131545754 @default.
• W4214938378 cites W2150228362 @default.
• W4214938378 cites W2167406761 @default.
• W4214938378 cites W2254267781 @default.
• W4214938378 cites W2337259322 @default.
• W4214938378 cites W2387134834 @default.
• W4214938378 cites W2401404581 @default.
• W4214938378 cites W2513506562 @default.
• W4214938378 cites W2757805629 @default.
• W4214938378 cites W2891893703 @default.
• W4214938378 cites W2951242028 @default.
• W4214938378 cites W3092130198 @default.
• W4214938378 cites W3114943710 @default.
• W4214938378 cites W3200577577 @default.
• W4214938378 doi "https://doi.org/10.1111/2041-210x.13838" @default.
• W4214938378 hasPublicationYear "2022" @default.
• W4214938378 type Work @default.
• W4214938378 citedByCount "0" @default.
• W4214938378 crossrefType "journal-article" @default.
• W4214938378 hasAuthorship W4214938378A5071744559 @default.
• W4214938378 hasBestOaLocation W42149383781 @default.
• W4214938378 hasConcept C100906024 @default.
• W4214938378 hasConcept C105795698 @default.
• W4214938378 hasConcept C122048520 @default.
• W4214938378 hasConcept C12590798 @default.
• W4214938378 hasConcept C129848803 @default.
• W4214938378 hasConcept C185592680 @default.
• W4214938378 hasConcept C198531522 @default.
• W4214938378 hasConcept C199335787 @default.
• W4214938378 hasConcept C33923547 @default.
• W4214938378 hasConcept C41008148 @default.
• W4214938378 hasConcept C41054675 @default.
• W4214938378 hasConcept C43617362 @default.
• W4214938378 hasConceptScore W4214938378C100906024 @default.
• W4214938378 hasConceptScore W4214938378C105795698 @default.
• W4214938378 hasConceptScore W4214938378C122048520 @default.
• W4214938378 hasConceptScore W4214938378C12590798 @default.
• W4214938378 hasConceptScore W4214938378C129848803 @default.
• W4214938378 hasConceptScore W4214938378C185592680 @default.
• W4214938378 hasConceptScore W4214938378C198531522 @default.
• W4214938378 hasConceptScore W4214938378C199335787 @default.
• W4214938378 hasConceptScore W4214938378C33923547 @default.
• W4214938378 hasConceptScore W4214938378C41008148 @default.
• W4214938378 hasConceptScore W4214938378C41054675 @default.
• W4214938378 hasConceptScore W4214938378C43617362 @default.
• W4214938378 hasIssue "5" @default.
• W4214938378 hasLocation W42149383781 @default.
• W4214938378 hasLocation W42149383782 @default.
• W4214938378 hasOpenAccess W4214938378 @default.
• W4214938378 hasPrimaryLocation W42149383781 @default.
• W4214938378 hasRelatedWork W120285656 @default.
• W4214938378 hasRelatedWork W2037403216 @default.
• W4214938378 hasRelatedWork W2052110748 @default.
• W4214938378 hasRelatedWork W2390910791 @default.
• W4214938378 hasRelatedWork W2505382103 @default.
• W4214938378 hasRelatedWork W2887966696 @default.
• W4214938378 hasRelatedWork W4230873770 @default.
• W4214938378 hasRelatedWork W4244236455 @default.
• W4214938378 hasRelatedWork W4283077984 @default.
• W4214938378 hasRelatedWork W62389518 @default.
• W4214938378 hasVolume "13" @default.
• W4214938378 isParatext "false" @default.
• W4214938378 isRetracted "false" @default.
• W4214938378 workType "article" @default.