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- W4220653375 abstract "Abstract Understanding the composition of the oviduct fluid (OF) is crucial to better comprehend the microenvironment in which sperm capacitation, fertilization and early embryo development take place. Therefore, our aim was to determine the spatiotemporal changes in the OF proteome according to the anatomical region of the oviduct (ampulla vs. isthmus), the proximity of the ovulating ovary (ipsilateral vs . contralateral side) and the peri-ovulatory stage (pre-ovulatory or Pre-ov vs . post-ovulatory or Post-ov). Oviducts from adult cyclic cows were collected at a local slaughterhouse and pools of OF were analyzed by nanoLC-MS/MS and label-free protein quantification (n = 32 OF pools for all region × stage × side conditions). A total of 3760 proteins were identified in the OF, of which 65% were predicted to be potentially secreted. The oviduct region was the major source of variation in protein abundance, followed by the proximity of the ovulating ovary and finally the peri-ovulatory stage. Differentially abundant proteins between regions, stages and sides were involved in a broad variety of biological functions, including protein binding, response to stress, cell-to-cell adhesion, calcium homeostasis and the immune system. This work highlights the dynamic regulation of oviduct secretions and provides new protein candidates for interactions between the maternal environment, the gametes and the early embryo." @default.
- W4220653375 created "2022-04-03" @default.
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- W4220653375 date "2022-03-09" @default.
- W4220653375 modified "2023-10-18" @default.
- W4220653375 title "Spatiotemporal profiling of the bovine oviduct fluid proteome around the time of ovulation" @default.
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- W4220653375 doi "https://doi.org/10.1038/s41598-022-07929-3" @default.
- W4220653375 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35264682" @default.
- W4220653375 hasPublicationYear "2022" @default.
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