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- W4220653445 abstract "Article18 March 2022 Source DataTransparent process ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis Ting Song Ting Song orcid.org/0000-0001-5945-6676 Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, College of Life Sciences, Collaborative Innovation Center of Cell Biology in Universities of Shandong, Shandong Normal University, Jinan, China Contribution: Data curation, Validation, Investigation, Methodology, Writing - original draft, Writing - review & editing Search for more papers by this author Yunfan Yang Yunfan Yang orcid.org/0000-0002-5266-7208 Cheeloo College of Medicine, Shandong University, Jinan, China Contribution: Formal analysis, Investigation Search for more papers by this author Peng Zhou Peng Zhou Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, College of Life Sciences, Collaborative Innovation Center of Cell Biology in Universities of Shandong, Shandong Normal University, Jinan, China Contribution: Investigation Search for more papers by this author Jie Ran Jie Ran orcid.org/0000-0002-0744-7126 Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, College of Life Sciences, Collaborative Innovation Center of Cell Biology in Universities of Shandong, Shandong Normal University, Jinan, China Contribution: Investigation Search for more papers by this author Liang Zhang Liang Zhang Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, College of Life Sciences, Collaborative Innovation Center of Cell Biology in Universities of Shandong, Shandong Normal University, Jinan, China Contribution: Investigation, Methodology Search for more papers by this author Xiaofan Wu Xiaofan Wu State Key Laboratory of Medicinal Chemical Biology, Haihe Laboratory of Cell Ecology, Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin, China Contribution: Investigation Search for more papers by this author Wei Xie Wei Xie orcid.org/0000-0002-3844-3013 Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, College of Life Sciences, Collaborative Innovation Center of Cell Biology in Universities of Shandong, Shandong Normal University, Jinan, China Contribution: Investigation Search for more papers by this author Tao Zhong Tao Zhong orcid.org/0000-0003-3898-057X Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, College of Life Sciences, Collaborative Innovation Center of Cell Biology in Universities of Shandong, Shandong Normal University, Jinan, China Contribution: Investigation Search for more papers by this author Hongbin Liu Hongbin Liu orcid.org/0000-0003-2550-7492 Cheeloo College of Medicine, Shandong University, Jinan, China Search for more papers by this author Min Liu Min Liu Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, College of Life Sciences, Collaborative Innovation Center of Cell Biology in Universities of Shandong, Shandong Normal University, Jinan, China Contribution: Formal analysis Search for more papers by this author Dengwen Li Dengwen Li State Key Laboratory of Medicinal Chemical Biology, Haihe Laboratory of Cell Ecology, Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin, China Contribution: Data curation, Formal analysis Search for more papers by this author Huijie Zhao Corresponding Author Huijie Zhao [email protected] orcid.org/0000-0002-8595-8159 Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, College of Life Sciences, Collaborative Innovation Center of Cell Biology in Universities of Shandong, Shandong Normal University, Jinan, China Contribution: Data curation, Formal analysis, Writing - review & editing Search for more papers by this author Jun Zhou Corresponding Author Jun Zhou [email protected] orcid.org/0000-0003-3131-7804 Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, College of Life Sciences, Collaborative Innovation Center of Cell Biology in Universities of Shandong, Shandong Normal University, Jinan, China State Key Laboratory of Medicinal Chemical Biology, Haihe Laboratory of Cell Ecology, Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin, China Contribution: Conceptualization, Data curation, Formal analysis, Supervision, Funding acquisition, Writing - original draft, Writing - review & editing Search for more papers by this author Ting Song Ting Song orcid.org/0000-0001-5945-6676 Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, College of Life Sciences, Collaborative Innovation Center of Cell Biology in Universities of Shandong, Shandong Normal University, Jinan, China Contribution: Data curation, Validation, Investigation, Methodology, Writing - original draft, Writing - review & editing Search for more papers by this author Yunfan Yang Yunfan Yang orcid.org/0000-0002-5266-7208 Cheeloo College of Medicine, Shandong University, Jinan, China Contribution: Formal analysis, Investigation Search for more papers by this author Peng Zhou Peng Zhou Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, College of Life Sciences, Collaborative Innovation Center of Cell Biology in Universities of Shandong, Shandong Normal University, Jinan, China Contribution: Investigation Search for more papers by this author Jie Ran Jie Ran orcid.org/0000-0002-0744-7126 Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, College of Life Sciences, Collaborative Innovation Center of Cell Biology in Universities of Shandong, Shandong Normal University, Jinan, China Contribution: Investigation Search for more papers by this author Liang Zhang Liang Zhang Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, College of Life Sciences, Collaborative Innovation Center of Cell Biology in Universities of Shandong, Shandong Normal University, Jinan, China Contribution: Investigation, Methodology Search for more papers by this author Xiaofan Wu Xiaofan Wu State Key Laboratory of Medicinal Chemical Biology, Haihe Laboratory of Cell Ecology, Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin, China Contribution: Investigation Search for more papers by this author Wei Xie Wei Xie orcid.org/0000-0002-3844-3013 Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, College of Life Sciences, Collaborative Innovation Center of Cell Biology in Universities of Shandong, Shandong Normal University, Jinan, China Contribution: Investigation Search for more papers by this author Tao Zhong Tao Zhong orcid.org/0000-0003-3898-057X Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, College of Life Sciences, Collaborative Innovation Center of Cell Biology in Universities of Shandong, Shandong Normal University, Jinan, China Contribution: Investigation Search for more papers by this author Hongbin Liu Hongbin Liu orcid.org/0000-0003-2550-7492 Cheeloo College of Medicine, Shandong University, Jinan, China Search for more papers by this author Min Liu Min Liu Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, College of Life Sciences, Collaborative Innovation Center of Cell Biology in Universities of Shandong, Shandong Normal University, Jinan, China Contribution: Formal analysis Search for more papers by this author Dengwen Li Dengwen Li State Key Laboratory of Medicinal Chemical Biology, Haihe Laboratory of Cell Ecology, Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin, China Contribution: Data curation, Formal analysis Search for more papers by this author Huijie Zhao Corresponding Author Huijie Zhao [email protected] orcid.org/0000-0002-8595-8159 Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, College of Life Sciences, Collaborative Innovation Center of Cell Biology in Universities of Shandong, Shandong Normal University, Jinan, China Contribution: Data curation, Formal analysis, Writing - review & editing Search for more papers by this author Jun Zhou Corresponding Author Jun Zhou [email protected] orcid.org/0000-0003-3131-7804 Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, College of Life Sciences, Collaborative Innovation Center of Cell Biology in Universities of Shandong, Shandong Normal University, Jinan, China State Key Laboratory of Medicinal Chemical Biology, Haihe Laboratory of Cell Ecology, Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin, China Contribution: Conceptualization, Data curation, Formal analysis, Supervision, Funding acquisition, Writing - original draft, Writing - review & editing Search for more papers by this author Author Information Ting Song1,†, Yunfan Yang2,†, Peng Zhou1, Jie Ran1, Liang Zhang1, Xiaofan Wu3, Wei Xie1, Tao Zhong1, Hongbin Liu2, Min Liu1, Dengwen Li3, Huijie Zhao *,1 and Jun Zhou *,1,3 1Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, College of Life Sciences, Collaborative Innovation Center of Cell Biology in Universities of Shandong, Shandong Normal University, Jinan, China 2Cheeloo College of Medicine, Shandong University, Jinan, China 3State Key Laboratory of Medicinal Chemical Biology, Haihe Laboratory of Cell Ecology, Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin, China † These authors contributed equally to this work *Corresponding author. Tel: +86 531 8618 2518; E-mail: [email protected] *Corresponding author. Tel: +86 531 8618 2518; E-mail: [email protected] EMBO Reports (2022)23:e54090https://doi.org/10.15252/embr.202154090 Full textView the full text of the articlePDFDownload PDF of article text and main figures. Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Abstract Despite the importance of cilia in cell signaling and motility, the molecular mechanisms regulating cilium formation remain incompletely understood. Herein, we characterize enkurin domain-containing protein 1 (ENKD1) as a novel centrosomal protein that mediates the removal of centriolar coiled–coil protein 110 (CP110) from the mother centriole to promote ciliogenesis. We show that Enkd1 knockout mice possess ciliogenesis defects in multiple organs. Super-resolution microscopy reveals that ENKD1 is a stable component of the centrosome throughout the ciliogenesis process. Simultaneous knockdown of ENKD1 and CP110 significantly reverses the ciliogenesis defects induced by ENKD1 depletion. Protein interaction analysis shows that ENKD1 competes with centrosomal protein 97 (CEP97) in binding to CP110. Depletion of ENKD1 enhances the CP110–CEP97 interaction and detains CP110 at the mother centriole. These findings thus identify ENKD1 as a centrosomal protein and uncover a novel mechanism controlling CP110 removal from the mother centriole for the initiation of ciliogenesis. SYNOPSIS ENKD1 is a centrosomal protein and initiates mother centriole uncapping through competing with CEP97 in binding to CP110. Loss of ENKD1 in mice causes multi-organ developmental abnormalities due to ciliogenesis defects. Depletion of ENKD1 in mice impairs the formation of both primary and motile cilia in multiple organs. ENKD1 localizes to the centrosome and interacts with CP110 directly to license mother centriole uncapping. Depletion of ENKD1 enhances the CP110-CEP97 interaction and inhibits CP110 removal from the mother centriole. Previous ArticleNext Article Read MoreAbout the coverClose modalView large imageVolume 23,Issue 5,04 May 2022This month's cover highlights the article Melanopsin elevates locomotor activity during the wake state of the diurnal zebrafish by Marcus Dekens, Kristin Tessmar-Raible and colleagues, showing that the light activated non-visual photoreceptor Melanopsin (Opn4) represses genes encoding melatonin synthesis enzymes in the pineal and thereby regulates daytime swimming velocity in the diurnal zebrafish. The cover image shows a closeup of a sagittal section through the wild-type mature zebrafish brain, with the epiphysis cerebri or pineal gland (red) on top of the telencephalon (red: dopa decarboxylase mRNA; blue: nuclei; green: actin). The pineal gland produces melatonin (floating molecule), the indicator of darkness. (Confocal image by Marcus Dekens and Marko Pende, Vienna BioCenter. Copyright: Marcus Dekens.) Volume 23Issue 54 May 2022In this issue RelatedDetailsLoading ..." @default.
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