FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4220656504> ?p ?o ?g. }

• W4220656504 abstract "The SARS-CoV-2 non-structural protein 1 (Nsp1) contains an N-terminal domain and C-terminal helices connected by a short linker region. The C-terminal helices of Nsp1 (Nsp1-C-ter) from SARS-CoV-2 bind in the mRNA entry channel of the 40S ribosomal subunit and blocks mRNA entry, thereby shutting down host protein synthesis. Nsp1 suppresses host immune function and is vital for viral replication. Hence, Nsp1 appears to be an attractive target for therapeutics. In this study, we have in silico screened Food and Drug Administration (FDA)-approved drugs against Nsp1-C-ter. Among the top hits obtained, montelukast sodium hydrate binds to Nsp1 with a binding affinity (KD) of 10.8 ± 0.2 µM in vitro. It forms a stable complex with Nsp1-C-ter in simulation runs with -95.8 ± 13.3 kJ/mol binding energy. Montelukast sodium hydrate also rescues the inhibitory effect of Nsp1 in host protein synthesis, as demonstrated by the expression of firefly luciferase reporter gene in cells. Importantly, it shows antiviral activity against SARS-CoV-2 with reduced viral replication in HEK cells expressing ACE2 and Vero-E6 cells. We, therefore, propose montelukast sodium hydrate can be used as a lead molecule to design potent inhibitors to help combat SARS-CoV-2 infection." @default.
• W4220656504 created "2022-04-03" @default.
• W4220656504 creator A5008856869 @default.
• W4220656504 creator A5024409024 @default.
• W4220656504 creator A5026180362 @default.
• W4220656504 creator A5039624597 @default.
• W4220656504 creator A5056835086 @default.
• W4220656504 creator A5056883794 @default.
• W4220656504 creator A5062177977 @default.
• W4220656504 creator A5062448717 @default.
• W4220656504 creator A5071201248 @default.
• W4220656504 date "2022-03-24" @default.
• W4220656504 modified "2023-10-05" @default.
• W4220656504 title "Drug targeting Nsp1-ribosomal complex shows antiviral activity against SARS-CoV-2" @default.
• W4220656504 cites W1974809008 @default.
• W4220656504 cites W1995816895 @default.
• W4220656504 cites W2132629607 @default.
• W4220656504 cites W2139942857 @default.
• W4220656504 cites W2155991787 @default.
• W4220656504 cites W2156095183 @default.
• W4220656504 cites W2404280981 @default.
• W4220656504 cites W3022285953 @default.
• W4220656504 cites W3035628751 @default.
• W4220656504 cites W3043427328 @default.
• W4220656504 cites W3083872269 @default.
• W4220656504 cites W3084071750 @default.
• W4220656504 cites W3087078168 @default.
• W4220656504 cites W3087534897 @default.
• W4220656504 cites W3089955663 @default.
• W4220656504 cites W3092040438 @default.
• W4220656504 cites W3094854996 @default.
• W4220656504 cites W3096967708 @default.
• W4220656504 cites W3097585503 @default.
• W4220656504 cites W3108368875 @default.
• W4220656504 cites W3111400428 @default.
• W4220656504 cites W3126798852 @default.
• W4220656504 cites W3127173946 @default.
• W4220656504 cites W3133012596 @default.
• W4220656504 cites W3202287440 @default.
• W4220656504 cites W3202880756 @default.
• W4220656504 cites W4255677297 @default.
• W4220656504 doi "https://doi.org/10.7554/elife.74877" @default.
• W4220656504 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35323109" @default.
• W4220656504 hasPublicationYear "2022" @default.
• W4220656504 type Work @default.
• W4220656504 citedByCount "24" @default.
• W4220656504 countsByYear W42206565042022 @default.
• W4220656504 countsByYear W42206565042023 @default.
• W4220656504 crossrefType "journal-article" @default.
• W4220656504 hasAuthorship W4220656504A5008856869 @default.
• W4220656504 hasAuthorship W4220656504A5024409024 @default.
• W4220656504 hasAuthorship W4220656504A5026180362 @default.
• W4220656504 hasAuthorship W4220656504A5039624597 @default.
• W4220656504 hasAuthorship W4220656504A5056835086 @default.
• W4220656504 hasAuthorship W4220656504A5056883794 @default.
• W4220656504 hasAuthorship W4220656504A5062177977 @default.
• W4220656504 hasAuthorship W4220656504A5062448717 @default.
• W4220656504 hasAuthorship W4220656504A5071201248 @default.
• W4220656504 hasBestOaLocation W42206565041 @default.
• W4220656504 hasConcept C104317684 @default.
• W4220656504 hasConcept C111335760 @default.
• W4220656504 hasConcept C140704245 @default.
• W4220656504 hasConcept C159047783 @default.
• W4220656504 hasConcept C174510640 @default.
• W4220656504 hasConcept C185592680 @default.
• W4220656504 hasConcept C202751555 @default.
• W4220656504 hasConcept C2522874641 @default.
• W4220656504 hasConcept C2776997796 @default.
• W4220656504 hasConcept C54009773 @default.
• W4220656504 hasConcept C55493867 @default.
• W4220656504 hasConcept C58475186 @default.
• W4220656504 hasConcept C86803240 @default.
• W4220656504 hasConcept C89138240 @default.
• W4220656504 hasConcept C95444343 @default.
• W4220656504 hasConceptScore W4220656504C104317684 @default.
• W4220656504 hasConceptScore W4220656504C111335760 @default.
• W4220656504 hasConceptScore W4220656504C140704245 @default.
• W4220656504 hasConceptScore W4220656504C159047783 @default.
• W4220656504 hasConceptScore W4220656504C174510640 @default.
• W4220656504 hasConceptScore W4220656504C185592680 @default.
• W4220656504 hasConceptScore W4220656504C202751555 @default.
• W4220656504 hasConceptScore W4220656504C2522874641 @default.
• W4220656504 hasConceptScore W4220656504C2776997796 @default.
• W4220656504 hasConceptScore W4220656504C54009773 @default.
• W4220656504 hasConceptScore W4220656504C55493867 @default.
• W4220656504 hasConceptScore W4220656504C58475186 @default.
• W4220656504 hasConceptScore W4220656504C86803240 @default.
• W4220656504 hasConceptScore W4220656504C89138240 @default.
• W4220656504 hasConceptScore W4220656504C95444343 @default.
• W4220656504 hasFunder F4320325580 @default.
• W4220656504 hasFunder F4320336200 @default.
• W4220656504 hasLocation W42206565041 @default.
• W4220656504 hasLocation W42206565042 @default.
• W4220656504 hasLocation W42206565043 @default.
• W4220656504 hasLocation W42206565044 @default.
• W4220656504 hasLocation W42206565045 @default.
• W4220656504 hasOpenAccess W4220656504 @default.
• W4220656504 hasPrimaryLocation W42206565041 @default.
• W4220656504 hasRelatedWork W1563852122 @default.
• W4220656504 hasRelatedWork W2008041430 @default.

• next