Matches in SemOpenAlex for { <https://semopenalex.org/work/W4220664009> ?p ?o ?g. }
- W4220664009 endingPage "104074" @default.
- W4220664009 startingPage "104074" @default.
- W4220664009 abstract "Genetic instability of Chinese hamster ovary (CHO) cells is implicated in production inconsistency through poorly defined mechanisms. Using a multi-omics approach, we analyzed the variations of CHO lineages derived from CHO-K1 cells. We identify an equilibrium between random genetic variation of the CHO genome and heritable traits driven by culture conditions, selection criteria, and genetic linkage. These inherited changes are associated with the selection pressures related to serum removal, suspension culture transition, protein expression, and secretion. We observed that a haploid reduction of a Chromosome 2 region after serum-free, suspension adaptation, was consistently inherited, suggesting common adaptation mechanisms. Genetic variations also included ∼200 insertions/deletions, ∼1000 single-nucleotide polymorphisms, and ∼300-2000 copy number variations, which were exacerbated after gene editing. In addition, heterochromatic chromosomes were preferentially lost as cells continuously evolved. Together, these observations demonstrate a highly plastic signature for adapted CHO cells and paves the way towards future host cell engineering." @default.
- W4220664009 created "2022-04-03" @default.
- W4220664009 creator A5002405425 @default.
- W4220664009 creator A5004392744 @default.
- W4220664009 creator A5013747946 @default.
- W4220664009 creator A5014510444 @default.
- W4220664009 creator A5015434378 @default.
- W4220664009 creator A5029470931 @default.
- W4220664009 creator A5043615443 @default.
- W4220664009 creator A5045435869 @default.
- W4220664009 creator A5079505187 @default.
- W4220664009 date "2022-04-01" @default.
- W4220664009 modified "2023-09-25" @default.
- W4220664009 title "Chromosomal instability drives convergent and divergent evolution toward advantageous inherited traits in mammalian CHO bioproduction lineages" @default.
- W4220664009 cites W1485133218 @default.
- W4220664009 cites W1531821806 @default.
- W4220664009 cites W1967142377 @default.
- W4220664009 cites W1970244111 @default.
- W4220664009 cites W1976570893 @default.
- W4220664009 cites W2027783748 @default.
- W4220664009 cites W2033433083 @default.
- W4220664009 cites W2035640095 @default.
- W4220664009 cites W2048941695 @default.
- W4220664009 cites W2049762689 @default.
- W4220664009 cites W2056025078 @default.
- W4220664009 cites W2062634373 @default.
- W4220664009 cites W2065960941 @default.
- W4220664009 cites W2072880634 @default.
- W4220664009 cites W2082745201 @default.
- W4220664009 cites W2093343938 @default.
- W4220664009 cites W2103441770 @default.
- W4220664009 cites W2105393513 @default.
- W4220664009 cites W2108234281 @default.
- W4220664009 cites W2113812682 @default.
- W4220664009 cites W2118118130 @default.
- W4220664009 cites W2127173397 @default.
- W4220664009 cites W2130410032 @default.
- W4220664009 cites W2137662702 @default.
- W4220664009 cites W2151655117 @default.
- W4220664009 cites W2168619204 @default.
- W4220664009 cites W2169509924 @default.
- W4220664009 cites W2171632740 @default.
- W4220664009 cites W2192080449 @default.
- W4220664009 cites W2277014805 @default.
- W4220664009 cites W2292162760 @default.
- W4220664009 cites W2337209831 @default.
- W4220664009 cites W2340837533 @default.
- W4220664009 cites W2597047713 @default.
- W4220664009 cites W2603712266 @default.
- W4220664009 cites W2607237411 @default.
- W4220664009 cites W2783705227 @default.
- W4220664009 cites W2793630204 @default.
- W4220664009 cites W2802589296 @default.
- W4220664009 cites W2883378801 @default.
- W4220664009 cites W2886706087 @default.
- W4220664009 cites W2895888195 @default.
- W4220664009 cites W2901331365 @default.
- W4220664009 cites W2936665613 @default.
- W4220664009 cites W2953352762 @default.
- W4220664009 cites W2993181268 @default.
- W4220664009 cites W3004898466 @default.
- W4220664009 cites W3168638715 @default.
- W4220664009 cites W4221030787 @default.
- W4220664009 cites W2995942898 @default.
- W4220664009 doi "https://doi.org/10.1016/j.isci.2022.104074" @default.
- W4220664009 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35355517" @default.
- W4220664009 hasPublicationYear "2022" @default.
- W4220664009 type Work @default.
- W4220664009 citedByCount "4" @default.
- W4220664009 countsByYear W42206640092022 @default.
- W4220664009 countsByYear W42206640092023 @default.
- W4220664009 crossrefType "journal-article" @default.
- W4220664009 hasAuthorship W4220664009A5002405425 @default.
- W4220664009 hasAuthorship W4220664009A5004392744 @default.
- W4220664009 hasAuthorship W4220664009A5013747946 @default.
- W4220664009 hasAuthorship W4220664009A5014510444 @default.
- W4220664009 hasAuthorship W4220664009A5015434378 @default.
- W4220664009 hasAuthorship W4220664009A5029470931 @default.
- W4220664009 hasAuthorship W4220664009A5043615443 @default.
- W4220664009 hasAuthorship W4220664009A5045435869 @default.
- W4220664009 hasAuthorship W4220664009A5079505187 @default.
- W4220664009 hasBestOaLocation W42206640091 @default.
- W4220664009 hasConcept C104317684 @default.
- W4220664009 hasConcept C127716648 @default.
- W4220664009 hasConcept C139807058 @default.
- W4220664009 hasConcept C140752955 @default.
- W4220664009 hasConcept C169760540 @default.
- W4220664009 hasConcept C175656101 @default.
- W4220664009 hasConcept C30481170 @default.
- W4220664009 hasConcept C54355233 @default.
- W4220664009 hasConcept C81885089 @default.
- W4220664009 hasConcept C86803240 @default.
- W4220664009 hasConceptScore W4220664009C104317684 @default.
- W4220664009 hasConceptScore W4220664009C127716648 @default.
- W4220664009 hasConceptScore W4220664009C139807058 @default.
- W4220664009 hasConceptScore W4220664009C140752955 @default.
- W4220664009 hasConceptScore W4220664009C169760540 @default.
- W4220664009 hasConceptScore W4220664009C175656101 @default.