Matches in SemOpenAlex for { <https://semopenalex.org/work/W4220672381> ?p ?o ?g. }
- W4220672381 endingPage "6582" @default.
- W4220672381 startingPage "6575" @default.
- W4220672381 abstract "DNA nanotechnology has been widely employed in the construction of various functional nanostructures. However, most DNA nanostructures rely on hybridization between multiple single-stranded DNAs. Herein, we report a general strategy for the construction of a double-stranded DNA-ribonucleoprotein (RNP) hybrid nanostructure by folding double-stranded DNA with a covalently bivalent clustered regularly interspaced short palindromic repeats (CRISPR)/nuclease-dead CRISPR-associated protein (dCas) system. In our design, dCas9 and dCas12a can be efficiently fused together through a flexible and stimuli-responsive peptide linker. After activation by guide RNAs, the covalently bivalent dCas9-12a RNPs (staples) can precisely recognize their target sequences in the double-stranded DNA scaffold and pull them together to construct a series of double-stranded DNA-RNP hybrid nanostructures. The genetically encoded hybrid nanostructure can protect genetic information in the folded state, similar to the natural DNA-protein hybrids present in chromosomes, and elicit efficient stimuli-responsive gene transcription in the unfolded form. This rationally developed double-stranded DNA folding and unfolding strategy presents a new avenue for the development of DNA nanotechnology." @default.
- W4220672381 created "2022-04-03" @default.
- W4220672381 creator A5014898916 @default.
- W4220672381 creator A5017941291 @default.
- W4220672381 creator A5018252358 @default.
- W4220672381 creator A5020271615 @default.
- W4220672381 creator A5023951892 @default.
- W4220672381 creator A5041071784 @default.
- W4220672381 creator A5044236517 @default.
- W4220672381 creator A5057653396 @default.
- W4220672381 creator A5060956929 @default.
- W4220672381 creator A5067232661 @default.
- W4220672381 creator A5077796273 @default.
- W4220672381 creator A5091165765 @default.
- W4220672381 date "2022-03-31" @default.
- W4220672381 modified "2023-10-14" @default.
- W4220672381 title "Genetically Encoded Double-Stranded DNA-Based Nanostructure Folded by a Covalently Bivalent CRISPR/dCas System" @default.
- W4220672381 cites W1572579115 @default.
- W4220672381 cites W1667902255 @default.
- W4220672381 cites W1971630118 @default.
- W4220672381 cites W1972759536 @default.
- W4220672381 cites W1974231617 @default.
- W4220672381 cites W1975679096 @default.
- W4220672381 cites W1976135176 @default.
- W4220672381 cites W1976815031 @default.
- W4220672381 cites W1982969769 @default.
- W4220672381 cites W2003171404 @default.
- W4220672381 cites W2006391873 @default.
- W4220672381 cites W2019403558 @default.
- W4220672381 cites W2038156304 @default.
- W4220672381 cites W2042330028 @default.
- W4220672381 cites W2045435533 @default.
- W4220672381 cites W2045692387 @default.
- W4220672381 cites W2048055667 @default.
- W4220672381 cites W2056512830 @default.
- W4220672381 cites W2064815984 @default.
- W4220672381 cites W2065518020 @default.
- W4220672381 cites W2068427957 @default.
- W4220672381 cites W2076446466 @default.
- W4220672381 cites W2083582836 @default.
- W4220672381 cites W2089589904 @default.
- W4220672381 cites W2094296077 @default.
- W4220672381 cites W2099737063 @default.
- W4220672381 cites W2103409481 @default.
- W4220672381 cites W2107959600 @default.
- W4220672381 cites W2112444085 @default.
- W4220672381 cites W2117817585 @default.
- W4220672381 cites W2126961173 @default.
- W4220672381 cites W2140022328 @default.
- W4220672381 cites W2142515819 @default.
- W4220672381 cites W2145375806 @default.
- W4220672381 cites W2156179144 @default.
- W4220672381 cites W2156764325 @default.
- W4220672381 cites W2158346543 @default.
- W4220672381 cites W2160051211 @default.
- W4220672381 cites W2165869860 @default.
- W4220672381 cites W2269042829 @default.
- W4220672381 cites W2297087890 @default.
- W4220672381 cites W2324839412 @default.
- W4220672381 cites W2337251054 @default.
- W4220672381 cites W2341405505 @default.
- W4220672381 cites W2346386833 @default.
- W4220672381 cites W2534583847 @default.
- W4220672381 cites W2583624671 @default.
- W4220672381 cites W2598407534 @default.
- W4220672381 cites W2598607484 @default.
- W4220672381 cites W2736242246 @default.
- W4220672381 cites W2757764473 @default.
- W4220672381 cites W2765257165 @default.
- W4220672381 cites W2768169611 @default.
- W4220672381 cites W2772823114 @default.
- W4220672381 cites W2778806019 @default.
- W4220672381 cites W2883718213 @default.
- W4220672381 cites W2935346656 @default.
- W4220672381 cites W2947834670 @default.
- W4220672381 cites W2986966530 @default.
- W4220672381 cites W2989653485 @default.
- W4220672381 cites W3010215023 @default.
- W4220672381 cites W3026872952 @default.
- W4220672381 cites W3029876292 @default.
- W4220672381 cites W3034772251 @default.
- W4220672381 cites W3037346400 @default.
- W4220672381 cites W3083445928 @default.
- W4220672381 cites W4211087439 @default.
- W4220672381 cites W4239521868 @default.
- W4220672381 doi "https://doi.org/10.1021/jacs.2c01760" @default.
- W4220672381 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35357193" @default.
- W4220672381 hasPublicationYear "2022" @default.
- W4220672381 type Work @default.
- W4220672381 citedByCount "7" @default.
- W4220672381 countsByYear W42206723812022 @default.
- W4220672381 countsByYear W42206723812023 @default.
- W4220672381 crossrefType "journal-article" @default.
- W4220672381 hasAuthorship W4220672381A5014898916 @default.
- W4220672381 hasAuthorship W4220672381A5017941291 @default.
- W4220672381 hasAuthorship W4220672381A5018252358 @default.
- W4220672381 hasAuthorship W4220672381A5020271615 @default.
- W4220672381 hasAuthorship W4220672381A5023951892 @default.