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- W4220693543 abstract "Wounding evokes transient increases in cytosolic calcium (Ca2+) concentration. Visualizing real-time Ca2+ flux provides new insights into Ca2+-signaling pathways. Here, we outline a protocol to detect insect feeding-induced Ca2+ flux elevation in Nicotiana benthamiana leaves based on the GCaMP3 reporter system by Leica fluorescence stereo microscopes (LFSM). LFSM combines super-fast manual screening with high-end imaging capabilities. Through this protocol, we can clearly observe the calcium flow after aphid's piercing-sucking. Additionally, we describe a protocol to quantify Ca2+ level using LFSM. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021)." @default.
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- W4220693543 date "2022-03-01" @default.
- W4220693543 modified "2023-10-11" @default.
- W4220693543 title "Live imaging and quantitation of insect feeding-induced Ca2+ signal using GCaMP3-based system in Nicotiana benthamiana" @default.
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- W4220693543 doi "https://doi.org/10.1016/j.xpro.2021.101040" @default.
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