FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4220700557> ?p ?o ?g. }

• W4220700557 endingPage "232" @default.
• W4220700557 startingPage "232" @default.
• W4220700557 abstract "Disintegrin-like/cysteine-rich (DC) proteins have long been regarded just as products of proteolysis of P-III snake venom metalloproteinases (SVMPs). However, here we demonstrate that a DC protein from the venom of Vipera ammodytes (Vaa; nose-horned viper), VaaMPIII-3, is encoded per se by a P-III SVMP-like gene that has a deletion in the region of the catalytic metalloproteinase domain and in part of the non-catalytic disintegrin-like domain. In this way, we justify the proposal of the introduction of a new subclass P-IIIe of SVMP-derived DC proteins. We purified VaaMPIII-3 from the venom of Vaa in a series of chromatographic steps. A covalent chromatography step based on thiol-disulphide exchange revealed that VaaMPIII-3 contains an unpaired Cys residue. This was demonstrated to be Cys6 in about 90% and Cys19 in about 10% of the VaaMPIII-3 molecules. We further constructed a three-dimensional homology model of VaaMPIII-3. From this model, it is evident that both Cys6 and Cys19 can pair with Cys26, which suggests that the intramolecular thiol-disulphide exchange has a regulatory function. VaaMPIII-3 is an acidic 21-kDa monomeric glycoprotein that exists in at least six N-glycoforms, with isoelectric points ranging from pH 4.5 to 5.1. Consistent with the presence of an integrin-binding motif in its sequence, SECD, VaaMPIII-3 inhibited collagen-induced platelet aggregation. It also inhibited ADP- and arachidonic-acid-induced platelet aggregation, but not ristocetin-induced platelet agglutination and the blood coagulation cascade." @default.
• W4220700557 created "2022-04-03" @default.
• W4220700557 creator A5004552048 @default.
• W4220700557 creator A5013588356 @default.
• W4220700557 creator A5018357671 @default.
• W4220700557 creator A5034321058 @default.
• W4220700557 creator A5035550045 @default.
• W4220700557 creator A5039907428 @default.
• W4220700557 creator A5040154329 @default.
• W4220700557 creator A5045898861 @default.
• W4220700557 creator A5046868078 @default.
• W4220700557 creator A5047291517 @default.
• W4220700557 creator A5051008509 @default.
• W4220700557 date "2022-03-23" @default.
• W4220700557 modified "2023-10-12" @default.
• W4220700557 title "Genomic Confirmation of the P-IIIe Subclass of Snake Venom Metalloproteinases and Characterisation of Its First Member, a Disintegrin-Like/Cysteine-Rich Protein" @default.
• W4220700557 cites W1565563859 @default.
• W4220700557 cites W1939750459 @default.
• W4220700557 cites W1965696475 @default.
• W4220700557 cites W1977613157 @default.
• W4220700557 cites W1984267713 @default.
• W4220700557 cites W1986191025 @default.
• W4220700557 cites W1986203517 @default.
• W4220700557 cites W1987960747 @default.
• W4220700557 cites W1994928637 @default.
• W4220700557 cites W1997274277 @default.
• W4220700557 cites W2002713035 @default.
• W4220700557 cites W2003446792 @default.
• W4220700557 cites W2007635285 @default.
• W4220700557 cites W2009942259 @default.
• W4220700557 cites W2014137199 @default.
• W4220700557 cites W2018289835 @default.
• W4220700557 cites W2024292436 @default.
• W4220700557 cites W2029408615 @default.
• W4220700557 cites W2036681912 @default.
• W4220700557 cites W2040470885 @default.
• W4220700557 cites W2043383369 @default.
• W4220700557 cites W2044516757 @default.
• W4220700557 cites W2044534848 @default.
• W4220700557 cites W2050964385 @default.
• W4220700557 cites W2055043387 @default.
• W4220700557 cites W2055785948 @default.
• W4220700557 cites W2061792920 @default.
• W4220700557 cites W2065283382 @default.
• W4220700557 cites W2073555092 @default.
• W4220700557 cites W2089723741 @default.
• W4220700557 cites W2094061973 @default.
• W4220700557 cites W2100837269 @default.
• W4220700557 cites W2102610356 @default.
• W4220700557 cites W2103546835 @default.
• W4220700557 cites W2112687576 @default.
• W4220700557 cites W2122101604 @default.
• W4220700557 cites W2126369283 @default.
• W4220700557 cites W2136731274 @default.
• W4220700557 cites W2150789905 @default.
• W4220700557 cites W2286797526 @default.
• W4220700557 cites W234460068 @default.
• W4220700557 cites W2398288095 @default.
• W4220700557 cites W2405975433 @default.
• W4220700557 cites W2437320864 @default.
• W4220700557 cites W2527520038 @default.
• W4220700557 cites W2604743104 @default.
• W4220700557 cites W2740138340 @default.
• W4220700557 cites W2764233378 @default.
• W4220700557 cites W2767033333 @default.
• W4220700557 cites W2789677262 @default.
• W4220700557 cites W2808618567 @default.
• W4220700557 cites W2923030606 @default.
• W4220700557 cites W2938574745 @default.
• W4220700557 cites W2941462697 @default.
• W4220700557 cites W2946628877 @default.
• W4220700557 cites W3013667857 @default.
• W4220700557 cites W3017879639 @default.
• W4220700557 cites W3021745639 @default.
• W4220700557 cites W3173003106 @default.
• W4220700557 cites W3173624910 @default.
• W4220700557 cites W4238990083 @default.
• W4220700557 cites W4376453254 @default.
• W4220700557 doi "https://doi.org/10.3390/toxins14040232" @default.
• W4220700557 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35448841" @default.
• W4220700557 hasPublicationYear "2022" @default.
• W4220700557 type Work @default.
• W4220700557 citedByCount "5" @default.
• W4220700557 countsByYear W42207005572022 @default.
• W4220700557 countsByYear W42207005572023 @default.
• W4220700557 crossrefType "journal-article" @default.
• W4220700557 hasAuthorship W4220700557A5004552048 @default.
• W4220700557 hasAuthorship W4220700557A5013588356 @default.
• W4220700557 hasAuthorship W4220700557A5018357671 @default.
• W4220700557 hasAuthorship W4220700557A5034321058 @default.
• W4220700557 hasAuthorship W4220700557A5035550045 @default.
• W4220700557 hasAuthorship W4220700557A5039907428 @default.
• W4220700557 hasAuthorship W4220700557A5040154329 @default.
• W4220700557 hasAuthorship W4220700557A5045898861 @default.
• W4220700557 hasAuthorship W4220700557A5046868078 @default.
• W4220700557 hasAuthorship W4220700557A5047291517 @default.
• W4220700557 hasAuthorship W4220700557A5051008509 @default.
• W4220700557 hasBestOaLocation W42207005571 @default.

• next