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• W4220722511 startingPage "4247" @default.
• W4220722511 abstract "Nucleic acid amplification strategies have successfully dominated ultrasensitive bioassays, but they sometimes bring high time-consumption, multi-step operation, increased contamination risk, and mismatch-related inaccuracy. We proposed a nucleic acid amplification-free method called the AuNPs-tagging based CRISPR-Cas12a bioassay platform. The signal amplification was realized by integrating the self-amplification effect of CRISPR-Cas12a with the enhancement effect of the large number of detectable atoms inside each gold nanoparticle. The proposed method achieved a low LOD of 1.05 amol in 40 min for HIV-related DNA." @default.
• W4220722511 created "2022-04-03" @default.
• W4220722511 creator A5008549662 @default.
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• W4220722511 date "2022-01-01" @default.
• W4220722511 modified "2023-10-12" @default.
• W4220722511 title "Dual-amplified CRISPR-Cas12a bioassay for HIV-related nucleic acids" @default.
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• W4220722511 doi "https://doi.org/10.1039/d2cc00792d" @default.
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• W4220722511 hasPublicationYear "2022" @default.
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