Matches in SemOpenAlex for { <https://semopenalex.org/work/W4220723399> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W4220723399 abstract "Abstract Background We investigate the effect of long non-coding RNA (lncRNA) CCDC26 on the chemosensitivity of HCC cells to cisplatin and the underlying mechanisms. Methods Firstly, the expression of CCDC26 in HCC tissues was detected. After siRNA interference of CCDC26, the HCC cells QGY8105 and HCC9204 were selected as the objects of study to detect the changes in their chemosensitivity to cisplatin through the terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL) and IC50 assays. Additionally, the RT-PCR assay, RNA hybrid software analysis, and luciferase reporter assay were performed to screen and verify the miRNAs that showed endogenous competitive relationship with CCDC26. Finally, miRNA was co-transfected with CCDC26, so as to explore whether the competitive binding of CCDC26 with miRNA changed the sensitivities of QGY8105 and HCC9204 cells to cisplatin. t-test or analysis of variance was adopted to compare data between groups based on the data property.. Results CCDC26 expression in HCC tissues was higher than that in normal tissues. Under the action of cisplatin, the IC50 value in si-CCDC26 group was lower than that in si-NC group, while the percentage of TUNEL-positive cells was higher than that in si-NC group, and the differences were statistically significant. RT-PCR results indicated that, miR-340 expression in si-CCDC26 group was up-regulated relative to that in si-NC group; meanwhile, RNA hybrid analysis and luciferase verification results demonstrated that there was base complementation between CCDC26 and the “seed” region of miR-340. Thereafter, miR-340 was co-transfected with CCDC26 into HCC9204 cells, and it was discovered that, the IC50 value in miR-340 + CCDC26-wild type group increased compared with those in miR-340 + NC and miR-340 + CCDC26-mutant groups. Meanwhile, the percentage of TUNEL-positive cells was reduced, and the differences were of statistical significance. Conclusions LncRNA CCDC26 can reduce the chemosensitivity of HCC cells to cisplatin through the endogenous competitive binding with miR-340." @default.
- W4220723399 created "2022-04-03" @default.
- W4220723399 creator A5000280795 @default.
- W4220723399 creator A5029934907 @default.
- W4220723399 date "2022-03-11" @default.
- W4220723399 modified "2023-09-29" @default.
- W4220723399 title "Effect of Competitive Binding of Long Non-coding RNA CCDC26 with miR-340 on the Chemosensitivity of HCC Cells to Cisplatin" @default.
- W4220723399 doi "https://doi.org/10.21203/rs.3.rs-1433014/v1" @default.
- W4220723399 hasPublicationYear "2022" @default.
- W4220723399 type Work @default.
- W4220723399 citedByCount "0" @default.
- W4220723399 crossrefType "posted-content" @default.
- W4220723399 hasAuthorship W4220723399A5000280795 @default.
- W4220723399 hasAuthorship W4220723399A5029934907 @default.
- W4220723399 hasBestOaLocation W42207233991 @default.
- W4220723399 hasConcept C104317684 @default.
- W4220723399 hasConcept C111335760 @default.
- W4220723399 hasConcept C145059251 @default.
- W4220723399 hasConcept C153911025 @default.
- W4220723399 hasConcept C166703698 @default.
- W4220723399 hasConcept C185592680 @default.
- W4220723399 hasConcept C190283241 @default.
- W4220723399 hasConcept C196795494 @default.
- W4220723399 hasConcept C202751555 @default.
- W4220723399 hasConcept C22615655 @default.
- W4220723399 hasConcept C2776694085 @default.
- W4220723399 hasConcept C2777752497 @default.
- W4220723399 hasConcept C2778239845 @default.
- W4220723399 hasConcept C502942594 @default.
- W4220723399 hasConcept C54009773 @default.
- W4220723399 hasConcept C54355233 @default.
- W4220723399 hasConcept C55493867 @default.
- W4220723399 hasConcept C67705224 @default.
- W4220723399 hasConcept C81885089 @default.
- W4220723399 hasConcept C86803240 @default.
- W4220723399 hasConceptScore W4220723399C104317684 @default.
- W4220723399 hasConceptScore W4220723399C111335760 @default.
- W4220723399 hasConceptScore W4220723399C145059251 @default.
- W4220723399 hasConceptScore W4220723399C153911025 @default.
- W4220723399 hasConceptScore W4220723399C166703698 @default.
- W4220723399 hasConceptScore W4220723399C185592680 @default.
- W4220723399 hasConceptScore W4220723399C190283241 @default.
- W4220723399 hasConceptScore W4220723399C196795494 @default.
- W4220723399 hasConceptScore W4220723399C202751555 @default.
- W4220723399 hasConceptScore W4220723399C22615655 @default.
- W4220723399 hasConceptScore W4220723399C2776694085 @default.
- W4220723399 hasConceptScore W4220723399C2777752497 @default.
- W4220723399 hasConceptScore W4220723399C2778239845 @default.
- W4220723399 hasConceptScore W4220723399C502942594 @default.
- W4220723399 hasConceptScore W4220723399C54009773 @default.
- W4220723399 hasConceptScore W4220723399C54355233 @default.
- W4220723399 hasConceptScore W4220723399C55493867 @default.
- W4220723399 hasConceptScore W4220723399C67705224 @default.
- W4220723399 hasConceptScore W4220723399C81885089 @default.
- W4220723399 hasConceptScore W4220723399C86803240 @default.
- W4220723399 hasLocation W42207233991 @default.
- W4220723399 hasOpenAccess W4220723399 @default.
- W4220723399 hasPrimaryLocation W42207233991 @default.
- W4220723399 hasRelatedWork W11602745 @default.
- W4220723399 hasRelatedWork W11924396 @default.
- W4220723399 hasRelatedWork W1269684 @default.
- W4220723399 hasRelatedWork W13839765 @default.
- W4220723399 hasRelatedWork W16190833 @default.
- W4220723399 hasRelatedWork W4086413 @default.
- W4220723399 hasRelatedWork W5918727 @default.
- W4220723399 hasRelatedWork W6656056 @default.
- W4220723399 hasRelatedWork W8972232 @default.
- W4220723399 hasRelatedWork W2097050 @default.
- W4220723399 isParatext "false" @default.
- W4220723399 isRetracted "false" @default.
- W4220723399 workType "article" @default.