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• W4220731618 abstract "New COVID-19 treatments are desperately needed as case numbers continue to rise and emergent strains threaten vaccine efficacy. Cell therapy has revolutionized cancer treatment and holds much promise in combatting infectious disease, including COVID-19. Invariant natural killer T (iNKT) cells are a rare subset of T cells with potent antiviral and immunoregulatory functions and an excellent safety profile. Current iNKT cell strategies are hindered by the extremely low presence of iNKT cells, and we have developed a platform to overcome this critical limitation.We produced allogeneic HSC-engineered iNKT (AlloHSC-iNKT) cells through TCR engineering of human cord blood CD34+ hematopoietic stem cells (HSCs) and differentiation of these HSCs into iNKT cells in an Ex Vivo HSC-Derived iNKT Cell Culture. We then established in vitro SARS-CoV-2 infection assays to assess AlloHSC-iNKT cell antiviral and anti-hyperinflammation functions. Lastly, using in vitro and in vivo preclinical models, we evaluated AlloHSC-iNKT cell safety and immunogenicity for off-the-shelf application.We reliably generated AlloHSC-iNKT cells at high-yield and of high-purity; these resulting cells closely resembled endogenous human iNKT cells in phenotypes and functionalities. In cell culture, AlloHSC-iNKT cells directly killed SARS-CoV-2 infected cells and also selectively eliminated SARS-CoV-2 infection-stimulated inflammatory monocytes. In an in vitro mixed lymphocyte reaction (MLR) assay and an NSG mouse xenograft model, AlloHSC-iNKT cells were resistant to T cell-mediated alloreaction and did not cause GvHD.Here, we report a method to robustly produce therapeutic levels of AlloHSC-iNKT cells. Preclinical studies showed that these AlloHSC-iNKT cells closely resembled endogenous human iNKT cells, could reduce SARS-CoV-2 virus infection load and mitigate virus infection-induced hyperinflammation, and meanwhile were free of GvHD-risk and resistant to T cell-mediated allorejection. These results support the development of AlloHSC-iNKT cells as a promising off-the-shelf cell product for treating COVID-19; such a cell product has the potential to target the new emerging SARS-CoV-2 variants as well as the future new emerging viruses." @default.
• W4220731618 created "2022-04-03" @default.
• W4220731618 creator A5013483419 @default.
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• W4220731618 creator A5082181088 @default.
• W4220731618 creator A5083626954 @default.
• W4220731618 date "2022-03-21" @default.
• W4220731618 modified "2023-10-11" @default.
• W4220731618 title "Development of off-the-shelf hematopoietic stem cell-engineered invariant natural killer T cells for COVID-19 therapeutic intervention" @default.
• W4220731618 cites W1971186387 @default.
• W4220731618 cites W1990417936 @default.
• W4220731618 cites W1990602451 @default.
• W4220731618 cites W1990757618 @default.
• W4220731618 cites W1991494425 @default.
• W4220731618 cites W1996085011 @default.
• W4220731618 cites W2001686432 @default.
• W4220731618 cites W2006728042 @default.
• W4220731618 cites W2008886674 @default.
• W4220731618 cites W2025208239 @default.
• W4220731618 cites W2031449139 @default.
• W4220731618 cites W2031579881 @default.
• W4220731618 cites W2038123972 @default.
• W4220731618 cites W2049306835 @default.
• W4220731618 cites W2052605694 @default.
• W4220731618 cites W2060320549 @default.
• W4220731618 cites W2065709904 @default.
• W4220731618 cites W2075364390 @default.
• W4220731618 cites W2093443553 @default.
• W4220731618 cites W2095446449 @default.
• W4220731618 cites W2100213157 @default.
• W4220731618 cites W2118599195 @default.
• W4220731618 cites W2121477549 @default.
• W4220731618 cites W2136795220 @default.
• W4220731618 cites W2150797171 @default.
• W4220731618 cites W2167094013 @default.
• W4220731618 cites W2172029694 @default.
• W4220731618 cites W2256756668 @default.
• W4220731618 cites W2262576767 @default.
• W4220731618 cites W2295770513 @default.
• W4220731618 cites W2548694234 @default.
• W4220731618 cites W2604331373 @default.
• W4220731618 cites W2605463821 @default.
• W4220731618 cites W2626153771 @default.
• W4220731618 cites W2754562068 @default.
• W4220731618 cites W2755114342 @default.
• W4220731618 cites W2789366434 @default.
• W4220731618 cites W2793229570 @default.
• W4220731618 cites W2794045287 @default.
• W4220731618 cites W2802731336 @default.
• W4220731618 cites W2807110328 @default.
• W4220731618 cites W2811357073 @default.
• W4220731618 cites W2909946034 @default.
• W4220731618 cites W2913078898 @default.
• W4220731618 cites W2921443070 @default.
• W4220731618 cites W2924578117 @default.
• W4220731618 cites W2936355201 @default.
• W4220731618 cites W2943335541 @default.
• W4220731618 cites W2950627686 @default.
• W4220731618 cites W2969042755 @default.
• W4220731618 cites W2971734370 @default.
• W4220731618 cites W2987013001 @default.
• W4220731618 cites W2998345757 @default.
• W4220731618 cites W3001446400 @default.
• W4220731618 cites W3003465021 @default.
• W4220731618 cites W3005212621 @default.
• W4220731618 cites W3006703916 @default.
• W4220731618 cites W3008443627 @default.
• W4220731618 cites W3009932851 @default.
• W4220731618 cites W3014050022 @default.
• W4220731618 cites W3014249633 @default.
• W4220731618 cites W3018517500 @default.
• W4220731618 cites W3021880084 @default.
• W4220731618 cites W3022878099 @default.
• W4220731618 cites W3025129816 @default.
• W4220731618 cites W3025232799 @default.
• W4220731618 cites W3037058363 @default.
• W4220731618 cites W3037768701 @default.
• W4220731618 cites W3038028872 @default.
• W4220731618 cites W3047402958 @default.
• W4220731618 cites W3081770779 @default.
• W4220731618 cites W3091504395 @default.
• W4220731618 cites W3106878713 @default.
• W4220731618 cites W3111255098 @default.
• W4220731618 cites W3122702917 @default.
• W4220731618 cites W3123175540 @default.
• W4220731618 cites W3124144456 @default.
• W4220731618 cites W3132080445 @default.
• W4220731618 cites W3135297450 @default.
• W4220731618 cites W3144086685 @default.
• W4220731618 cites W3153508314 @default.
• W4220731618 cites W3159147687 @default.
• W4220731618 cites W3165605711 @default.

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