Matches in SemOpenAlex for { <https://semopenalex.org/work/W4220753179> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W4220753179 endingPage "769" @default.
- W4220753179 startingPage "761" @default.
- W4220753179 abstract "Enterocytozoon hepatopenaei (EHP) and Vibrio parahaemolyticus acute hepatopancreatic necrosis disease (VPAHPND) are two of the diseases that have frequently infected farmed shrimp in recent years, causing great economic losses to the shrimp industry worldwide. In this study, we established a sensitive and accurate duplex droplet digital PCR (ddPCR) method that can simultaneously detect and quantify the two pathogens simultaneously. The results showed that the ddPCR methods could detect EHP and VPAHPND specifically. The sensitivity levels of ddPCR for EHP and VPAHPND were 2.3 copies/μl and 4.6 copies/μl, respectively, which were 10-fold higher than the sensitivity of the qPCR assay and showed good reproducibility. Twenty-six suspected diseased shrimp samples were used for practical determination. For EHP, the detection rates of ddPCR and qPCR were 53.84% and 42.31%, respectively; for VPAHPND, the detection rates of ddPCR and qPCR were both 23.08%. The results indicated that the ddPCR method shows superiority for detection in samples with low viral loads, which will facilitate monitoring of the source and transmission of EHP and VPAHPND and will help control shrimp epidemic disease." @default.
- W4220753179 created "2022-04-03" @default.
- W4220753179 creator A5007123441 @default.
- W4220753179 creator A5044436054 @default.
- W4220753179 creator A5045951427 @default.
- W4220753179 creator A5049065453 @default.
- W4220753179 creator A5056088603 @default.
- W4220753179 creator A5065222546 @default.
- W4220753179 date "2022-03-24" @default.
- W4220753179 modified "2023-09-29" @default.
- W4220753179 title "Duplex droplet digital PCR method for the detection of <i>Enterocytozoon hepatopenaei</i> and <i>Vibrio parahaemolyticus</i> acute hepatopancreatic necrosis disease" @default.
- W4220753179 cites W1546271565 @default.
- W4220753179 cites W1997195261 @default.
- W4220753179 cites W2087402699 @default.
- W4220753179 cites W2165654685 @default.
- W4220753179 cites W2172377009 @default.
- W4220753179 cites W2205778302 @default.
- W4220753179 cites W2513245969 @default.
- W4220753179 cites W2517598398 @default.
- W4220753179 cites W2554303099 @default.
- W4220753179 cites W2570030766 @default.
- W4220753179 cites W2571284194 @default.
- W4220753179 cites W2808781047 @default.
- W4220753179 cites W2896440671 @default.
- W4220753179 cites W2909264316 @default.
- W4220753179 cites W2910412604 @default.
- W4220753179 cites W2983284972 @default.
- W4220753179 cites W2997672651 @default.
- W4220753179 cites W3001559900 @default.
- W4220753179 cites W3033453640 @default.
- W4220753179 cites W3132678056 @default.
- W4220753179 cites W3135910230 @default.
- W4220753179 doi "https://doi.org/10.1111/jfd.13600" @default.
- W4220753179 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35322884" @default.
- W4220753179 hasPublicationYear "2022" @default.
- W4220753179 type Work @default.
- W4220753179 citedByCount "1" @default.
- W4220753179 countsByYear W42207531792023 @default.
- W4220753179 crossrefType "journal-article" @default.
- W4220753179 hasAuthorship W4220753179A5007123441 @default.
- W4220753179 hasAuthorship W4220753179A5044436054 @default.
- W4220753179 hasAuthorship W4220753179A5045951427 @default.
- W4220753179 hasAuthorship W4220753179A5049065453 @default.
- W4220753179 hasAuthorship W4220753179A5056088603 @default.
- W4220753179 hasAuthorship W4220753179A5065222546 @default.
- W4220753179 hasConcept C104317684 @default.
- W4220753179 hasConcept C159047783 @default.
- W4220753179 hasConcept C2776575720 @default.
- W4220753179 hasConcept C2776841996 @default.
- W4220753179 hasConcept C2987315675 @default.
- W4220753179 hasConcept C42972112 @default.
- W4220753179 hasConcept C48023723 @default.
- W4220753179 hasConcept C48126324 @default.
- W4220753179 hasConcept C49105822 @default.
- W4220753179 hasConcept C505870484 @default.
- W4220753179 hasConcept C523546767 @default.
- W4220753179 hasConcept C54355233 @default.
- W4220753179 hasConcept C71924100 @default.
- W4220753179 hasConcept C86803240 @default.
- W4220753179 hasConcept C89423630 @default.
- W4220753179 hasConceptScore W4220753179C104317684 @default.
- W4220753179 hasConceptScore W4220753179C159047783 @default.
- W4220753179 hasConceptScore W4220753179C2776575720 @default.
- W4220753179 hasConceptScore W4220753179C2776841996 @default.
- W4220753179 hasConceptScore W4220753179C2987315675 @default.
- W4220753179 hasConceptScore W4220753179C42972112 @default.
- W4220753179 hasConceptScore W4220753179C48023723 @default.
- W4220753179 hasConceptScore W4220753179C48126324 @default.
- W4220753179 hasConceptScore W4220753179C49105822 @default.
- W4220753179 hasConceptScore W4220753179C505870484 @default.
- W4220753179 hasConceptScore W4220753179C523546767 @default.
- W4220753179 hasConceptScore W4220753179C54355233 @default.
- W4220753179 hasConceptScore W4220753179C71924100 @default.
- W4220753179 hasConceptScore W4220753179C86803240 @default.
- W4220753179 hasConceptScore W4220753179C89423630 @default.
- W4220753179 hasFunder F4320321001 @default.
- W4220753179 hasIssue "6" @default.
- W4220753179 hasLocation W42207531791 @default.
- W4220753179 hasLocation W42207531792 @default.
- W4220753179 hasOpenAccess W4220753179 @default.
- W4220753179 hasPrimaryLocation W42207531791 @default.
- W4220753179 hasRelatedWork W1984613240 @default.
- W4220753179 hasRelatedWork W2196023150 @default.
- W4220753179 hasRelatedWork W2331239048 @default.
- W4220753179 hasRelatedWork W2625939968 @default.
- W4220753179 hasRelatedWork W2784372986 @default.
- W4220753179 hasRelatedWork W2905706750 @default.
- W4220753179 hasRelatedWork W2951084549 @default.
- W4220753179 hasRelatedWork W3040441071 @default.
- W4220753179 hasRelatedWork W3160513946 @default.
- W4220753179 hasRelatedWork W4303438007 @default.
- W4220753179 hasVolume "45" @default.
- W4220753179 isParatext "false" @default.
- W4220753179 isRetracted "false" @default.
- W4220753179 workType "article" @default.