Matches in SemOpenAlex for { <https://semopenalex.org/work/W4220754411> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W4220754411 endingPage "13" @default.
- W4220754411 startingPage "1" @default.
- W4220754411 abstract "Pepper yellow leaf curl Indonesia virus (PepYLCIV) is an important pathogen on chili cultivation and is transmitted through the seed. Serological tests are sensitive, accurate, efficient and it has been widely used for the detection of seed-transmitted plant viruses. This study aimed to produce PepYLCIV recombinant protein as a material to produce recombinant antibodies PepYLCIV. DNA was extracted from infected chili leaves collected from Congkrang, Muntilan, Central Java verified using primer PepYLCIV-BamHI and PepYLCIV-EcoRI and produced an amplicon at 840 bp. The amplified fragments were cloned into the pET32a then transformed to Escherichia coli BL21. The percentage of nucleotide sequence identity and sequence of amino acid, PepYLCIV CK-6 isolates had the highest similarity of nucleotide and amino acid sequences to of chili isolates from Bandung. The expressed recombinant protein was obtained with IPTG concentration 0,5 mM and harvested at 6 hours after IPTG induction. SDS PAGE analysis of the recombinant plasmid Begomovirus CK-6 showed that the coat protein size was about 29 kDa. Immunization was carried out on rabbits by injecting 150 µg of recombinant protein 4 times with an interval of 1 week to produce crude antiserum and pure antiserum capable of detecting PepYLCIV in chili and Ageratum conyzoides using I-ELISA and DIBA tests." @default.
- W4220754411 created "2022-04-03" @default.
- W4220754411 creator A5023761848 @default.
- W4220754411 creator A5043082247 @default.
- W4220754411 creator A5051837653 @default.
- W4220754411 creator A5052042493 @default.
- W4220754411 creator A5058572310 @default.
- W4220754411 creator A5082080926 @default.
- W4220754411 creator A5083012813 @default.
- W4220754411 date "2022-03-01" @default.
- W4220754411 modified "2023-10-14" @default.
- W4220754411 title "RECOMBINANT ANTIBODY PRODUCTION BY CLONING OF Pepper yellow leaf curl Indonesia virus (PepYLCIV) COAT PROTEIN GENE" @default.
- W4220754411 doi "https://doi.org/10.23960/jhptt.1221-13" @default.
- W4220754411 hasPublicationYear "2022" @default.
- W4220754411 type Work @default.
- W4220754411 citedByCount "0" @default.
- W4220754411 crossrefType "journal-article" @default.
- W4220754411 hasAuthorship W4220754411A5023761848 @default.
- W4220754411 hasAuthorship W4220754411A5043082247 @default.
- W4220754411 hasAuthorship W4220754411A5051837653 @default.
- W4220754411 hasAuthorship W4220754411A5052042493 @default.
- W4220754411 hasAuthorship W4220754411A5058572310 @default.
- W4220754411 hasAuthorship W4220754411A5082080926 @default.
- W4220754411 hasAuthorship W4220754411A5083012813 @default.
- W4220754411 hasBestOaLocation W42207544111 @default.
- W4220754411 hasConcept C104317684 @default.
- W4220754411 hasConcept C128319531 @default.
- W4220754411 hasConcept C130123407 @default.
- W4220754411 hasConcept C153911025 @default.
- W4220754411 hasConcept C159047783 @default.
- W4220754411 hasConcept C159654299 @default.
- W4220754411 hasConcept C40767141 @default.
- W4220754411 hasConcept C54355233 @default.
- W4220754411 hasConcept C86803240 @default.
- W4220754411 hasConcept C91523082 @default.
- W4220754411 hasConceptScore W4220754411C104317684 @default.
- W4220754411 hasConceptScore W4220754411C128319531 @default.
- W4220754411 hasConceptScore W4220754411C130123407 @default.
- W4220754411 hasConceptScore W4220754411C153911025 @default.
- W4220754411 hasConceptScore W4220754411C159047783 @default.
- W4220754411 hasConceptScore W4220754411C159654299 @default.
- W4220754411 hasConceptScore W4220754411C40767141 @default.
- W4220754411 hasConceptScore W4220754411C54355233 @default.
- W4220754411 hasConceptScore W4220754411C86803240 @default.
- W4220754411 hasConceptScore W4220754411C91523082 @default.
- W4220754411 hasIssue "1" @default.
- W4220754411 hasLocation W42207544111 @default.
- W4220754411 hasOpenAccess W4220754411 @default.
- W4220754411 hasPrimaryLocation W42207544111 @default.
- W4220754411 hasRelatedWork W2024013673 @default.
- W4220754411 hasRelatedWork W2359256335 @default.
- W4220754411 hasRelatedWork W2363435241 @default.
- W4220754411 hasRelatedWork W2363768087 @default.
- W4220754411 hasRelatedWork W2376161078 @default.
- W4220754411 hasRelatedWork W2377374699 @default.
- W4220754411 hasRelatedWork W2390064643 @default.
- W4220754411 hasRelatedWork W3151417595 @default.
- W4220754411 hasRelatedWork W2331009388 @default.
- W4220754411 hasRelatedWork W2360392486 @default.
- W4220754411 hasVolume "22" @default.
- W4220754411 isParatext "false" @default.
- W4220754411 isRetracted "false" @default.
- W4220754411 workType "article" @default.