FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4220760023> ?p ?o ?g. }

• W4220760023 endingPage "246" @default.
• W4220760023 startingPage "237" @default.
• W4220760023 abstract "5-Carboxycytosine (5caC) is a rare epigenetic modification found in nucleic acids of all domains of life. Despite its sparse genomic abundance, 5caC is presumed to play essential regulatory roles in transcription, maintenance and base-excision processes in DNA. In this work, we utilize nuclear magnetic resonance (NMR) spectroscopy to address the effects of 5caC incorporation into canonical DNA strands at multiple pH and temperature conditions. Our results demonstrate that 5caC has a pH-dependent global destabilizing and a base-pair mobility enhancing local impact on dsDNA, albeit without any detectable influence on the ground-state B-DNA structure. Measurement of hybridization thermodynamics and kinetics of 5caC-bearing DNA duplexes highlighted how acidic environment (pH 5.8 and 4.7) destabilizes the double-stranded structure by ∼10–20 kJ mol–1 at 37 °C when compared to the same sample at neutral pH. Protonation of 5caC results in a lower activation energy for the dissociation process and a higher barrier for annealing. Studies on conformational exchange on the microsecond time scale regime revealed a sharply localized base-pair motion involving exclusively the modified site and its immediate surroundings. By direct comparison with canonical and 5-formylcytosine (5fC)-edited strands, we were able to address the impact of the two most oxidized naturally occurring cytosine derivatives in the genome. These insights on 5caC’s subtle sensitivity to acidic pH contribute to the long-standing questions of its capacity as a substrate in base excision repair processes and its purpose as an independent, stable epigenetic mark." @default.
• W4220760023 created "2022-04-03" @default.
• W4220760023 creator A5010773997 @default.
• W4220760023 creator A5026032684 @default.
• W4220760023 creator A5060135813 @default.
• W4220760023 creator A5063397487 @default.
• W4220760023 creator A5077398724 @default.
• W4220760023 creator A5091195461 @default.
• W4220760023 date "2022-02-11" @default.
• W4220760023 modified "2023-10-14" @default.
• W4220760023 title "¹H NMR Chemical Exchange Techniques Reveal Local and Global Effects of Oxidized Cytosine Derivatives" @default.
• W4220760023 cites W1503311137 @default.
• W4220760023 cites W1988326881 @default.
• W4220760023 cites W1989915883 @default.
• W4220760023 cites W1997453048 @default.
• W4220760023 cites W2025808344 @default.
• W4220760023 cites W2026257781 @default.
• W4220760023 cites W2051405525 @default.
• W4220760023 cites W2052246375 @default.
• W4220760023 cites W2059475138 @default.
• W4220760023 cites W2075151834 @default.
• W4220760023 cites W2076255761 @default.
• W4220760023 cites W2088650930 @default.
• W4220760023 cites W2106878505 @default.
• W4220760023 cites W2114716625 @default.
• W4220760023 cites W2132199053 @default.
• W4220760023 cites W2149902856 @default.
• W4220760023 cites W2157458089 @default.
• W4220760023 cites W2159538608 @default.
• W4220760023 cites W2246141446 @default.
• W4220760023 cites W2329142961 @default.
• W4220760023 cites W2329582709 @default.
• W4220760023 cites W2409146838 @default.
• W4220760023 cites W2476700496 @default.
• W4220760023 cites W2517327266 @default.
• W4220760023 cites W2517711883 @default.
• W4220760023 cites W2559026422 @default.
• W4220760023 cites W2592818030 @default.
• W4220760023 cites W2598979376 @default.
• W4220760023 cites W2599498000 @default.
• W4220760023 cites W2613613516 @default.
• W4220760023 cites W2617540636 @default.
• W4220760023 cites W2742757861 @default.
• W4220760023 cites W2757739379 @default.
• W4220760023 cites W2770066606 @default.
• W4220760023 cites W2793518245 @default.
• W4220760023 cites W2794665693 @default.
• W4220760023 cites W2799685692 @default.
• W4220760023 cites W2804090324 @default.
• W4220760023 cites W2808778957 @default.
• W4220760023 cites W2887367237 @default.
• W4220760023 cites W2904369742 @default.
• W4220760023 cites W2932082905 @default.
• W4220760023 cites W2939689244 @default.
• W4220760023 cites W2944683002 @default.
• W4220760023 cites W2950614992 @default.
• W4220760023 cites W2954804024 @default.
• W4220760023 cites W2995259006 @default.
• W4220760023 cites W2998474754 @default.
• W4220760023 cites W3004100947 @default.
• W4220760023 cites W3009668945 @default.
• W4220760023 cites W3036591694 @default.
• W4220760023 cites W3041756141 @default.
• W4220760023 cites W3134420600 @default.
• W4220760023 cites W3184055619 @default.
• W4220760023 doi "https://doi.org/10.1021/acsphyschemau.1c00050" @default.
• W4220760023 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35637781" @default.
• W4220760023 hasPublicationYear "2022" @default.
• W4220760023 type Work @default.
• W4220760023 citedByCount "3" @default.
• W4220760023 countsByYear W42207600232023 @default.
• W4220760023 crossrefType "journal-article" @default.
• W4220760023 hasAuthorship W4220760023A5010773997 @default.
• W4220760023 hasAuthorship W4220760023A5026032684 @default.
• W4220760023 hasAuthorship W4220760023A5060135813 @default.
• W4220760023 hasAuthorship W4220760023A5063397487 @default.
• W4220760023 hasAuthorship W4220760023A5077398724 @default.
• W4220760023 hasAuthorship W4220760023A5091195461 @default.
• W4220760023 hasBestOaLocation W42207600231 @default.
• W4220760023 hasConcept C121332964 @default.
• W4220760023 hasConcept C12554922 @default.
• W4220760023 hasConcept C129312508 @default.
• W4220760023 hasConcept C145148216 @default.
• W4220760023 hasConcept C147597530 @default.
• W4220760023 hasConcept C148898269 @default.
• W4220760023 hasConcept C178790620 @default.
• W4220760023 hasConcept C185581394 @default.
• W4220760023 hasConcept C185592680 @default.
• W4220760023 hasConcept C2776023528 @default.
• W4220760023 hasConcept C30095370 @default.
• W4220760023 hasConcept C552990157 @default.
• W4220760023 hasConcept C55493867 @default.
• W4220760023 hasConcept C59593255 @default.
• W4220760023 hasConcept C61234307 @default.
• W4220760023 hasConcept C62520636 @default.
• W4220760023 hasConcept C66974803 @default.
• W4220760023 hasConcept C71240020 @default.
• W4220760023 hasConcept C8010536 @default.

• next