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- W4220772681 abstract "Objectives: The present study was designed to investigate early proteome and phosphoproteome changes during inhibition of lymphocyte proliferation induced by sirolimus (SRL). Material and Methods: Proliferation assays were conducted using human CCRF-CEM T-lymphoblasts under different SRL concentrations. Total protein lysates after SRL treatment were used to distinguish significantly regulated proteins and phosphorylated proteins in 2DE gels using a Q-TOF Ultima Global mass spectrometer. Results and Discussion: Incubation with 2.5 µmol/l SRL resulted in an approx. 70% inhibition of cell proliferation. Cells incubated with 2.5 µmol/l for 30 min showed a differential phosphorylation pattern with one higher (TCPQ) and six lower-phosphorylation signals (TBA1B, VIME, HNRPD, ENPL, SEPT9, PLSL). On investigating the differential protein expression five proteins were found to be up- (ECHB, PSB3, MTDC, LDHB, NDKA) and four were down-regulated (EHD1, AATC, LMNB1, MDHC). Nine of these! differentially regulated proteins/phosphoproteins (TCPQ, TBA1B, VIME, HNRPD, ENPL, ECHB, PSB3, LDHB, and LMNB1) showed significant interaction potential through the binding protein YWHAZ using MINT software. Conclusions: In the present study the simultaneous early influence of SRL on the phosphorylation status and on the protein expression in the total proteome of CCRF-CEM-cells was reported for the first time. Nine proteins out of sixteen identified and significantly regulated proteins predicted interaction though the binding protein YWHAZ belonging to the 14-3-3 protein family." @default.
- W4220772681 created "2022-04-03" @default.
- W4220772681 creator A5058431567 @default.
- W4220772681 date "2022-02-20" @default.
- W4220772681 modified "2023-09-30" @default.
- W4220772681 title "Durch Sirolimus induzierte frühe Veränderungen des Proteoms und Phosphoproteoms in humanen T-Lymphoblasten" @default.
- W4220772681 doi "https://doi.org/10.53846/goediss-951" @default.
- W4220772681 hasPublicationYear "2022" @default.
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