Matches in SemOpenAlex for { <https://semopenalex.org/work/W4220783867> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W4220783867 endingPage "441" @default.
- W4220783867 startingPage "441" @default.
- W4220783867 abstract "Because of their high volumetric catalytic activity, in addition to their high chemical and thermal resistances, enzymes in the form of protein crystals are an excellent choice for application as immobilized biocatalysts. However, mechanical stability is a requirement for the processability of immobilisates, in addition to the protein crystals retaining their enzymatic activity, and this is closely related to the crystal structure. In this study, the influence of protein engineering on the mechanical stability of cross-linked enzyme crystals (CLECs) was investigated using a genetically modified model protein in which additionally cysteines were introduced on the protein surface for targeted cross-linking. The results showed that the mechanical stability of crystals of the mutant proteins in the native form was decreased compared to native wild-type crystals. However, specific cross-linking of the introduced amino acid residues in the mutant proteins resulted in their increased mechanical stability compared to wild-type CLECs. In order to determine the correlation between the crystal structure and the resulting mechanical properties of CLECs to enable targeted cross-linking, a previously developed model was revised and then used for the two model proteins. This model can explain the mechanically investigated relationships, such as the anisotropic crystal behavior and the influence of a linker or mutation on the micromechanical properties and, hence, can be helpful for the tailor-made production of CLECs." @default.
- W4220783867 created "2022-04-03" @default.
- W4220783867 creator A5014748300 @default.
- W4220783867 creator A5079943296 @default.
- W4220783867 creator A5082631864 @default.
- W4220783867 date "2022-03-22" @default.
- W4220783867 modified "2023-10-18" @default.
- W4220783867 title "Structure-Based Modeling of the Mechanical Behavior of Cross-Linked Enzyme Crystals" @default.
- W4220783867 cites W15436629 @default.
- W4220783867 cites W1652052270 @default.
- W4220783867 cites W1986802933 @default.
- W4220783867 cites W1987944388 @default.
- W4220783867 cites W2001290593 @default.
- W4220783867 cites W2009349362 @default.
- W4220783867 cites W2016909003 @default.
- W4220783867 cites W2017228994 @default.
- W4220783867 cites W2038323484 @default.
- W4220783867 cites W2049325327 @default.
- W4220783867 cites W2058678726 @default.
- W4220783867 cites W2066896815 @default.
- W4220783867 cites W2068447470 @default.
- W4220783867 cites W2068889098 @default.
- W4220783867 cites W2081565968 @default.
- W4220783867 cites W2121766097 @default.
- W4220783867 cites W2139722676 @default.
- W4220783867 cites W2148574622 @default.
- W4220783867 cites W2157599742 @default.
- W4220783867 cites W2158764764 @default.
- W4220783867 cites W2314503406 @default.
- W4220783867 cites W2607017924 @default.
- W4220783867 cites W2806782694 @default.
- W4220783867 cites W2891091061 @default.
- W4220783867 cites W2954333117 @default.
- W4220783867 cites W2956160671 @default.
- W4220783867 cites W3154625679 @default.
- W4220783867 cites W3176150383 @default.
- W4220783867 cites W4213264544 @default.
- W4220783867 cites W76878189 @default.
- W4220783867 doi "https://doi.org/10.3390/cryst12040441" @default.
- W4220783867 hasPublicationYear "2022" @default.
- W4220783867 type Work @default.
- W4220783867 citedByCount "4" @default.
- W4220783867 countsByYear W42207838672022 @default.
- W4220783867 countsByYear W42207838672023 @default.
- W4220783867 crossrefType "journal-article" @default.
- W4220783867 hasAuthorship W4220783867A5014748300 @default.
- W4220783867 hasAuthorship W4220783867A5079943296 @default.
- W4220783867 hasAuthorship W4220783867A5082631864 @default.
- W4220783867 hasBestOaLocation W42207838671 @default.
- W4220783867 hasConcept C104317684 @default.
- W4220783867 hasConcept C12554922 @default.
- W4220783867 hasConcept C143065580 @default.
- W4220783867 hasConcept C147816474 @default.
- W4220783867 hasConcept C153064111 @default.
- W4220783867 hasConcept C178790620 @default.
- W4220783867 hasConcept C181199279 @default.
- W4220783867 hasConcept C185592680 @default.
- W4220783867 hasConcept C192562407 @default.
- W4220783867 hasConcept C203036418 @default.
- W4220783867 hasConcept C55493867 @default.
- W4220783867 hasConcept C59061564 @default.
- W4220783867 hasConcept C8010536 @default.
- W4220783867 hasConcept C86803240 @default.
- W4220783867 hasConceptScore W4220783867C104317684 @default.
- W4220783867 hasConceptScore W4220783867C12554922 @default.
- W4220783867 hasConceptScore W4220783867C143065580 @default.
- W4220783867 hasConceptScore W4220783867C147816474 @default.
- W4220783867 hasConceptScore W4220783867C153064111 @default.
- W4220783867 hasConceptScore W4220783867C178790620 @default.
- W4220783867 hasConceptScore W4220783867C181199279 @default.
- W4220783867 hasConceptScore W4220783867C185592680 @default.
- W4220783867 hasConceptScore W4220783867C192562407 @default.
- W4220783867 hasConceptScore W4220783867C203036418 @default.
- W4220783867 hasConceptScore W4220783867C55493867 @default.
- W4220783867 hasConceptScore W4220783867C59061564 @default.
- W4220783867 hasConceptScore W4220783867C8010536 @default.
- W4220783867 hasConceptScore W4220783867C86803240 @default.
- W4220783867 hasFunder F4320320879 @default.
- W4220783867 hasIssue "4" @default.
- W4220783867 hasLocation W42207838671 @default.
- W4220783867 hasLocation W42207838672 @default.
- W4220783867 hasLocation W42207838673 @default.
- W4220783867 hasOpenAccess W4220783867 @default.
- W4220783867 hasPrimaryLocation W42207838671 @default.
- W4220783867 hasRelatedWork W1846454438 @default.
- W4220783867 hasRelatedWork W2052165960 @default.
- W4220783867 hasRelatedWork W2156414657 @default.
- W4220783867 hasRelatedWork W2613992936 @default.
- W4220783867 hasRelatedWork W2783070239 @default.
- W4220783867 hasRelatedWork W2784297255 @default.
- W4220783867 hasRelatedWork W2899084033 @default.
- W4220783867 hasRelatedWork W4245925619 @default.
- W4220783867 hasRelatedWork W4295096598 @default.
- W4220783867 hasRelatedWork W2036004033 @default.
- W4220783867 hasVolume "12" @default.
- W4220783867 isParatext "false" @default.
- W4220783867 isRetracted "false" @default.
- W4220783867 workType "article" @default.