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- W4220784575 endingPage "121473" @default.
- W4220784575 startingPage "121473" @default.
- W4220784575 abstract "Human cholangiocyte organoids are promising for regenerative medicine applications, such as repair of damaged bile ducts. However, organoids are typically cultured in mouse tumor-derived basement membrane extracts (BME), which is poorly defined, highly variable and limits the direct clinical applications of organoids in patients. Extracellular matrix (ECM)-derived hydrogels prepared from decellularized human or porcine livers are attractive alternative culture substrates. Here, the culture and expansion of human cholangiocyte organoids in liver ECM(LECM)-derived hydrogels is described. These hydrogels support proliferation of cholangiocyte organoids and maintain the cholangiocyte-like phenotype. The use of LECM hydrogels does not significantly alter the expression of selected genes or proteins, such as the cholangiocyte marker cytokeratin-7, and no species-specific effect is found between human or porcine LECM hydrogels. Proliferation rates of organoids cultured in LECM hydrogels are lower, but the differentiation capacity of the cholangiocyte organoids towards hepatocyte-like cells is not altered by the presence of tissue-specific ECM components. Moreover, human LECM extracts support the expansion of ICO in a dynamic culture set up without the need for laborious static culture of organoids in hydrogel domes. Liver ECM hydrogels can successfully replace tumor-derived BME and can potentially unlock the full clinical potential of human cholangiocyte organoids." @default.
- W4220784575 created "2022-04-03" @default.
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- W4220784575 date "2022-05-01" @default.
- W4220784575 modified "2023-10-13" @default.
- W4220784575 title "Hydrogels derived from decellularized liver tissue support the growth and differentiation of cholangiocyte organoids" @default.
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- W4220784575 cites W1561129928 @default.
- W4220784575 cites W1599568627 @default.
- W4220784575 cites W1971163936 @default.
- W4220784575 cites W1974144714 @default.
- W4220784575 cites W1997353326 @default.
- W4220784575 cites W1997547509 @default.
- W4220784575 cites W2042022714 @default.
- W4220784575 cites W2046338433 @default.
- W4220784575 cites W2048321002 @default.
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- W4220784575 cites W2060569139 @default.
- W4220784575 cites W2064297038 @default.
- W4220784575 cites W2074705887 @default.
- W4220784575 cites W2076704378 @default.
- W4220784575 cites W2085652695 @default.
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- W4220784575 cites W2329880197 @default.
- W4220784575 cites W2470369424 @default.
- W4220784575 cites W2474510574 @default.
- W4220784575 cites W2510238988 @default.
- W4220784575 cites W2552754421 @default.
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- W4220784575 cites W2780132385 @default.
- W4220784575 cites W2797381838 @default.
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- W4220784575 cites W2886315925 @default.
- W4220784575 cites W2904723019 @default.
- W4220784575 cites W2936493533 @default.
- W4220784575 cites W2939654433 @default.
- W4220784575 cites W2969990975 @default.
- W4220784575 cites W2972492746 @default.
- W4220784575 cites W2981167732 @default.
- W4220784575 cites W2983693859 @default.
- W4220784575 cites W2984382194 @default.
- W4220784575 cites W2986073722 @default.
- W4220784575 cites W2988904063 @default.
- W4220784575 cites W2989172784 @default.
- W4220784575 cites W2998532899 @default.
- W4220784575 cites W3000332038 @default.
- W4220784575 cites W3004287765 @default.
- W4220784575 cites W3007661294 @default.
- W4220784575 cites W3029563942 @default.
- W4220784575 cites W3034392967 @default.
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- W4220784575 doi "https://doi.org/10.1016/j.biomaterials.2022.121473" @default.
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- W4220784575 hasPublicationYear "2022" @default.
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