Matches in SemOpenAlex for { <https://semopenalex.org/work/W4220788928> ?p ?o ?g. }
- W4220788928 endingPage "896" @default.
- W4220788928 startingPage "883" @default.
- W4220788928 abstract "The quantification of α-synuclein aggregates has emerged as a promising biomarker for synucleinopathies. Assays that amplify and detect such aggregates have revealed the presence of seeding-competent species in biosamples of patients diagnosed with Parkinson's disease. However, multiple species, such as oligomers and amyloid fibrils, are formed during the aggregation of α-synuclein; these species are likely to coexist in biological samples, and thus it remains unclear which species(s) are contributing to the signal detected in seeding assays. To identify individual contributions to the amplification process, recombinant oligomers and preformed fibrils were produced and purified to characterize their individual biochemical and seeding potential. Here, we used single molecule spectroscopy to track the formation and purification of oligomers and fibrils at the single particle level and compare their respective seeding potential in an amplification assay. Single molecule detection validates that size-exclusion chromatography efficiently separates oligomers from fibrils. Oligomers were found to be seeding-competent, but our results reveal that their seeding behavior is very different compared to that of preformed fibrils, in our amplification assay. Overall, our data suggest that even a low number of preformed fibrils present in biosamples is likely to dominate the response in seeding assays." @default.
- W4220788928 created "2022-04-03" @default.
- W4220788928 creator A5005909199 @default.
- W4220788928 creator A5012177640 @default.
- W4220788928 creator A5038663511 @default.
- W4220788928 creator A5046356609 @default.
- W4220788928 creator A5071161068 @default.
- W4220788928 creator A5077923906 @default.
- W4220788928 date "2022-03-14" @default.
- W4220788928 modified "2023-09-26" @default.
- W4220788928 title "Single Molecule Fingerprinting Reveals Different Amplification Properties of α-Synuclein Oligomers and Preformed Fibrils in Seeding Assay" @default.
- W4220788928 cites W1496790952 @default.
- W4220788928 cites W1572679174 @default.
- W4220788928 cites W1965888147 @default.
- W4220788928 cites W1977977555 @default.
- W4220788928 cites W1995191747 @default.
- W4220788928 cites W1995247791 @default.
- W4220788928 cites W2031365272 @default.
- W4220788928 cites W2031964854 @default.
- W4220788928 cites W2046885195 @default.
- W4220788928 cites W2050786534 @default.
- W4220788928 cites W2055310246 @default.
- W4220788928 cites W2061995199 @default.
- W4220788928 cites W2065131501 @default.
- W4220788928 cites W2072427499 @default.
- W4220788928 cites W2088642285 @default.
- W4220788928 cites W2133272695 @default.
- W4220788928 cites W2145147096 @default.
- W4220788928 cites W2145954079 @default.
- W4220788928 cites W2150656456 @default.
- W4220788928 cites W2158089391 @default.
- W4220788928 cites W2164919571 @default.
- W4220788928 cites W2203648598 @default.
- W4220788928 cites W2336689217 @default.
- W4220788928 cites W2432301206 @default.
- W4220788928 cites W2438674992 @default.
- W4220788928 cites W2618281342 @default.
- W4220788928 cites W2782823547 @default.
- W4220788928 cites W2805954533 @default.
- W4220788928 cites W2808814503 @default.
- W4220788928 cites W2809885021 @default.
- W4220788928 cites W2906247002 @default.
- W4220788928 cites W2914482173 @default.
- W4220788928 cites W2921561422 @default.
- W4220788928 cites W2934999326 @default.
- W4220788928 cites W2955256212 @default.
- W4220788928 cites W2969680393 @default.
- W4220788928 cites W2979832008 @default.
- W4220788928 cites W2992675275 @default.
- W4220788928 cites W2995182141 @default.
- W4220788928 cites W2999229807 @default.
- W4220788928 cites W3003226137 @default.
- W4220788928 cites W3005252823 @default.
- W4220788928 cites W3008786370 @default.
- W4220788928 cites W3020087588 @default.
- W4220788928 cites W3020408550 @default.
- W4220788928 cites W3025486771 @default.
- W4220788928 cites W3033726128 @default.
- W4220788928 cites W3033847448 @default.
- W4220788928 cites W3040814993 @default.
- W4220788928 cites W3100056764 @default.
- W4220788928 cites W3109975779 @default.
- W4220788928 cites W3121625382 @default.
- W4220788928 cites W3130158832 @default.
- W4220788928 cites W3139395934 @default.
- W4220788928 cites W3174191273 @default.
- W4220788928 cites W3212865892 @default.
- W4220788928 doi "https://doi.org/10.1021/acschemneuro.1c00553" @default.
- W4220788928 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35286811" @default.
- W4220788928 hasPublicationYear "2022" @default.
- W4220788928 type Work @default.
- W4220788928 citedByCount "4" @default.
- W4220788928 countsByYear W42207889282023 @default.
- W4220788928 crossrefType "journal-article" @default.
- W4220788928 hasAuthorship W4220788928A5005909199 @default.
- W4220788928 hasAuthorship W4220788928A5012177640 @default.
- W4220788928 hasAuthorship W4220788928A5038663511 @default.
- W4220788928 hasAuthorship W4220788928A5046356609 @default.
- W4220788928 hasAuthorship W4220788928A5071161068 @default.
- W4220788928 hasAuthorship W4220788928A5077923906 @default.
- W4220788928 hasBestOaLocation W42207889281 @default.
- W4220788928 hasConcept C12554922 @default.
- W4220788928 hasConcept C136238340 @default.
- W4220788928 hasConcept C142724271 @default.
- W4220788928 hasConcept C179104552 @default.
- W4220788928 hasConcept C181199279 @default.
- W4220788928 hasConcept C185592680 @default.
- W4220788928 hasConcept C193363816 @default.
- W4220788928 hasConcept C27523624 @default.
- W4220788928 hasConcept C2777440831 @default.
- W4220788928 hasConcept C2777633098 @default.
- W4220788928 hasConcept C2779134260 @default.
- W4220788928 hasConcept C2779734285 @default.
- W4220788928 hasConcept C2781449126 @default.
- W4220788928 hasConcept C36248471 @default.
- W4220788928 hasConcept C43617362 @default.
- W4220788928 hasConcept C55493867 @default.
- W4220788928 hasConcept C6557445 @default.