FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4220800976> ?p ?o ?g. }

• W4220800976 abstract "The halophilic γ-proteobacterium Halomonas elongata DSM 2581 T thrives at salt concentrations well above 10 % NaCl (1.7 M NaCl). A well-known osmoregulatory mechanism is the accumulation of the compatible solute ectoine within the cell in response to osmotic stress. While ectoine accumulation is central to osmoregulation and promotes resistance to high salinity in halophilic bacteria, ectoine has this effect only to a much lesser extent in non-halophiles. We carried out transcriptome analysis of H. elongata grown on two different carbon sources (acetate or glucose), and low (0.17 M NaCl), medium (1 M), and high salinity (2 M) to identify additional mechanisms for adaptation to high saline environments. To avoid a methodological bias, the transcripts were evaluated by applying two methods, DESeq2 and Transcripts Per Million (TPM). The differentially transcribed genes in response to the available carbon sources and salt stress were then compared to the transcriptome profile of Chromohalobacter salexigens, a closely related moderate halophilic bacterium. Transcriptome profiling supports the notion that glucose is degraded via the cytoplasmic Entner-Doudoroff pathway, whereas the Embden-Meyerhoff-Parnas pathway is employed for gluconeogenesis. The machinery of oxidative phosphorylation in H. elongata and C. salexigens differs greatly from that of non-halophilic organisms, and electron flow can occur from quinone to oxygen along four alternative routes. Two of these pathways via cytochrome bo' and cytochrome bd quinol oxidases seem to be upregulated in salt stressed cells. Among the most highly regulated genes in H. elongata and C. salexigens are those encoding chemotaxis and motility proteins, with genes for chemotaxis and flagellar assembly severely downregulated at low salt concentrations. We also compared transcripts at low and high-salt stress (low growth rate) with transcripts at optimal salt concentration and found that the majority of regulated genes were down-regulated in stressed cells, including many genes involved in carbohydrate metabolism, while ribosome synthesis was up-regulated, which is in contrast to what is known from non-halophiles at slow growth. Finally, comparing the acidity of the cytoplasmic proteomes of non-halophiles, extreme halophiles and moderate halophiles suggests adaptation to an increased cytoplasmic ion concentration of H. elongata. Taken together, these results lead us to propose a model for salt tolerance in H. elongata where ion accumulation plays a greater role in salt tolerance than previously assumed." @default.
• W4220800976 created "2022-04-03" @default.
• W4220800976 creator A5013221314 @default.
• W4220800976 creator A5024866710 @default.
• W4220800976 creator A5040048649 @default.
• W4220800976 creator A5040121825 @default.
• W4220800976 creator A5060909244 @default.
• W4220800976 creator A5069622667 @default.
• W4220800976 creator A5075963429 @default.
• W4220800976 creator A5078757245 @default.
• W4220800976 date "2022-03-30" @default.
• W4220800976 modified "2023-10-17" @default.
• W4220800976 title "Adaptation to Varying Salinity in Halomonas elongata: Much More Than Ectoine Accumulation" @default.
• W4220800976 cites W1264478585 @default.
• W4220800976 cites W1552229797 @default.
• W4220800976 cites W1559116292 @default.
• W4220800976 cites W1580982499 @default.
• W4220800976 cites W1583328087 @default.
• W4220800976 cites W1596408507 @default.
• W4220800976 cites W1606892626 @default.
• W4220800976 cites W1724546921 @default.
• W4220800976 cites W1755377688 @default.
• W4220800976 cites W1876441925 @default.
• W4220800976 cites W1882272649 @default.
• W4220800976 cites W1901110641 @default.
• W4220800976 cites W1965543043 @default.
• W4220800976 cites W1966116114 @default.
• W4220800976 cites W1971632242 @default.
• W4220800976 cites W1977670534 @default.
• W4220800976 cites W1978289664 @default.
• W4220800976 cites W1983572566 @default.
• W4220800976 cites W1985161570 @default.
• W4220800976 cites W1986495460 @default.
• W4220800976 cites W1992626271 @default.
• W4220800976 cites W1993887172 @default.
• W4220800976 cites W1999114042 @default.
• W4220800976 cites W1999574084 @default.
• W4220800976 cites W2005989563 @default.
• W4220800976 cites W2007135866 @default.
• W4220800976 cites W2016165178 @default.
• W4220800976 cites W2025074367 @default.
• W4220800976 cites W2025578918 @default.
• W4220800976 cites W2033167108 @default.
• W4220800976 cites W2036310798 @default.
• W4220800976 cites W2042397938 @default.
• W4220800976 cites W2042998656 @default.
• W4220800976 cites W2043281233 @default.
• W4220800976 cites W2053129511 @default.
• W4220800976 cites W2056513301 @default.
• W4220800976 cites W2057470797 @default.
• W4220800976 cites W2063954352 @default.
• W4220800976 cites W2065888283 @default.
• W4220800976 cites W2070895247 @default.
• W4220800976 cites W2072825693 @default.
• W4220800976 cites W2085249999 @default.
• W4220800976 cites W2087094813 @default.
• W4220800976 cites W2092341931 @default.
• W4220800976 cites W2092554064 @default.
• W4220800976 cites W2093283980 @default.
• W4220800976 cites W2096348604 @default.
• W4220800976 cites W2096611550 @default.
• W4220800976 cites W2097067993 @default.
• W4220800976 cites W2097250648 @default.
• W4220800976 cites W2098570629 @default.
• W4220800976 cites W2099792315 @default.
• W4220800976 cites W2104296423 @default.
• W4220800976 cites W2107018762 @default.
• W4220800976 cites W2107379667 @default.
• W4220800976 cites W2108234281 @default.
• W4220800976 cites W2113529870 @default.
• W4220800976 cites W2114850508 @default.
• W4220800976 cites W2137265693 @default.
• W4220800976 cites W2145416275 @default.
• W4220800976 cites W2158718811 @default.
• W4220800976 cites W2159682622 @default.
• W4220800976 cites W2160902874 @default.
• W4220800976 cites W2165348270 @default.
• W4220800976 cites W2167708455 @default.
• W4220800976 cites W2168342464 @default.
• W4220800976 cites W2168936792 @default.
• W4220800976 cites W2170551349 @default.
• W4220800976 cites W2179438025 @default.
• W4220800976 cites W2180566168 @default.
• W4220800976 cites W2225557190 @default.
• W4220800976 cites W2278253772 @default.
• W4220800976 cites W2321872593 @default.
• W4220800976 cites W2573864607 @default.
• W4220800976 cites W2580767461 @default.
• W4220800976 cites W2601907406 @default.
• W4220800976 cites W2763411981 @default.
• W4220800976 cites W2792236141 @default.
• W4220800976 cites W2887737157 @default.
• W4220800976 cites W2909240409 @default.
• W4220800976 cites W2950796920 @default.
• W4220800976 cites W2952288650 @default.
• W4220800976 cites W2996576365 @default.
• W4220800976 cites W3016136518 @default.
• W4220800976 cites W3017000198 @default.
• W4220800976 cites W3088652192 @default.
• W4220800976 doi "https://doi.org/10.3389/fmicb.2022.846677" @default.

• next