FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4220805119> ?p ?o ?g. }

• W4220805119 endingPage "375.e5" @default.
• W4220805119 startingPage "365" @default.
• W4220805119 abstract "A major goal in synthetic biology is coordinating cellular behavior using cell-cell interactions; however, designing and testing complex genetic circuits that function only in large populations remains challenging. Although directed evolution has commonly supplemented rational design methods for synthetic gene circuits, this method relies on the efficient screening of mutant libraries for desired phenotypes. Recently, multiple techniques have been developed for identifying dynamic phenotypes from large, pooled libraries. These technologies have advanced library screening for single-cell, time-varying phenotypes but are currently incompatible with population-level phenotypes dependent on cell-cell communication. Here, we utilize directed mutagenesis and multiplexed microfluidics to develop an arrayed-screening workflow for dynamic, population-level genetic circuits. Specifically, we create a mutant library of an existing oscillator, the synchronized lysis circuit, and discover variants with different period-amplitude characteristics. Lastly, we utilize our screening workflow to construct a transcriptionally regulated synchronized oscillator that functions over long timescales. A record of this paper's transparent peer review process is included in the supplemental information." @default.
• W4220805119 created "2022-04-03" @default.
• W4220805119 creator A5020180332 @default.
• W4220805119 creator A5052721078 @default.
• W4220805119 creator A5052945096 @default.
• W4220805119 date "2022-05-01" @default.
• W4220805119 modified "2023-10-18" @default.
• W4220805119 title "Design, mutate, screen: Multiplexed creation and arrayed screening of synchronized genetic clocks" @default.
• W4220805119 cites W1816644759 @default.
• W4220805119 cites W1970893992 @default.
• W4220805119 cites W1971439989 @default.
• W4220805119 cites W1979159575 @default.
• W4220805119 cites W1983078492 @default.
• W4220805119 cites W1985161570 @default.
• W4220805119 cites W1988544600 @default.
• W4220805119 cites W1995421491 @default.
• W4220805119 cites W2001841300 @default.
• W4220805119 cites W2023363172 @default.
• W4220805119 cites W2028343312 @default.
• W4220805119 cites W2030632688 @default.
• W4220805119 cites W2036517506 @default.
• W4220805119 cites W2043632006 @default.
• W4220805119 cites W2046270994 @default.
• W4220805119 cites W2047360542 @default.
• W4220805119 cites W2071657256 @default.
• W4220805119 cites W2074565201 @default.
• W4220805119 cites W2078672252 @default.
• W4220805119 cites W2084210400 @default.
• W4220805119 cites W2097861808 @default.
• W4220805119 cites W2114228460 @default.
• W4220805119 cites W2123467717 @default.
• W4220805119 cites W2127399801 @default.
• W4220805119 cites W2132828256 @default.
• W4220805119 cites W2138835511 @default.
• W4220805119 cites W2140443340 @default.
• W4220805119 cites W2141564052 @default.
• W4220805119 cites W2328206864 @default.
• W4220805119 cites W2330245166 @default.
• W4220805119 cites W2480697384 @default.
• W4220805119 cites W2514632434 @default.
• W4220805119 cites W2620120516 @default.
• W4220805119 cites W2620445954 @default.
• W4220805119 cites W2625497715 @default.
• W4220805119 cites W2726013144 @default.
• W4220805119 cites W2746227774 @default.
• W4220805119 cites W2791991616 @default.
• W4220805119 cites W2795045314 @default.
• W4220805119 cites W2807350372 @default.
• W4220805119 cites W2886103315 @default.
• W4220805119 cites W2887001543 @default.
• W4220805119 cites W2899862051 @default.
• W4220805119 cites W2903194329 @default.
• W4220805119 cites W2952024710 @default.
• W4220805119 cites W2952738056 @default.
• W4220805119 cites W2988799915 @default.
• W4220805119 cites W2990995235 @default.
• W4220805119 cites W3002350431 @default.
• W4220805119 cites W3009326905 @default.
• W4220805119 cites W3092728598 @default.
• W4220805119 cites W3138215020 @default.
• W4220805119 cites W3160173019 @default.
• W4220805119 doi "https://doi.org/10.1016/j.cels.2022.02.005" @default.
• W4220805119 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35320733" @default.
• W4220805119 hasPublicationYear "2022" @default.
• W4220805119 type Work @default.
• W4220805119 citedByCount "6" @default.
• W4220805119 countsByYear W42208051192022 @default.
• W4220805119 countsByYear W42208051192023 @default.
• W4220805119 crossrefType "journal-article" @default.
• W4220805119 hasAuthorship W4220805119A5020180332 @default.
• W4220805119 hasAuthorship W4220805119A5052721078 @default.
• W4220805119 hasAuthorship W4220805119A5052945096 @default.
• W4220805119 hasBestOaLocation W42208051191 @default.
• W4220805119 hasConcept C104317684 @default.
• W4220805119 hasConcept C127716648 @default.
• W4220805119 hasConcept C143065580 @default.
• W4220805119 hasConcept C144024400 @default.
• W4220805119 hasConcept C149923435 @default.
• W4220805119 hasConcept C16318435 @default.
• W4220805119 hasConcept C177212765 @default.
• W4220805119 hasConcept C191908910 @default.
• W4220805119 hasConcept C19275194 @default.
• W4220805119 hasConcept C207177822 @default.
• W4220805119 hasConcept C2908647359 @default.
• W4220805119 hasConcept C41008148 @default.
• W4220805119 hasConcept C501734568 @default.
• W4220805119 hasConcept C54355233 @default.
• W4220805119 hasConcept C70721500 @default.
• W4220805119 hasConcept C76155785 @default.
• W4220805119 hasConcept C77088390 @default.
• W4220805119 hasConcept C86803240 @default.
• W4220805119 hasConcept C9418097 @default.
• W4220805119 hasConceptScore W4220805119C104317684 @default.
• W4220805119 hasConceptScore W4220805119C127716648 @default.
• W4220805119 hasConceptScore W4220805119C143065580 @default.
• W4220805119 hasConceptScore W4220805119C144024400 @default.
• W4220805119 hasConceptScore W4220805119C149923435 @default.
• W4220805119 hasConceptScore W4220805119C16318435 @default.

• next