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- W4220828122 abstract "ATP-dependent proteases (FtsH, Lon, and Clp family proteins) are ubiquitous in bacteria and play essential roles in numerous regulatory cell processes. Xanthomonas campestris pv. campestris is a Gram-negative pathogen that can cause black rot diseases in crucifers. The genome of X. campestris pv. campestris has several clp genes, namely, clpS, clpA, clpX, clpP, clpQ, and clpY. Among these genes, only clpX and clpP is known to be required for pathogenicity. Here, we focused on two uncharacterized clp genes (clpS and clpA) that encode the adaptor (ClpS) and ATPase subunit (ClpA) of the ClpAP protease complex. Transcriptional analysis revealed that the expression of clpS and clpA was growth phase-dependent and affected by the growth temperature. The inactivation of clpA, but not of clpS, resulted in susceptibility to high temperature and attenuated virulence in the host plant. The altered phenotypes of the clpA mutant could be complemented in trans. Site-directed mutagenesis revealed that K223 and K504 were the amino acid residues critical for ClpA function in heat tolerance. The protein expression profile shown by the clpA mutant in response to heat stress was different from that exhibited by the wild type. In summary, we characterized two clp genes (clpS and clpA) by examining their expression profiles and functions in different processes, including stress tolerance and pathogenicity. We demonstrated that clpS and clpA were expressed in a temperature-dependent manner and that clpA was required for the survival at high temperature and full virulence of X. campestris pv. campestris. This work represents the first time that clpS and clpA were characterized in Xanthomonas." @default.
- W4220828122 created "2022-04-03" @default.
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- W4220828122 creator A5028371490 @default.
- W4220828122 creator A5058251377 @default.
- W4220828122 creator A5079387960 @default.
- W4220828122 date "2022-03-23" @default.
- W4220828122 modified "2023-10-07" @default.
- W4220828122 title "Expression and function of clpS and clpA in Xanthomonas campestris pv. campestris" @default.
- W4220828122 cites W1508395921 @default.
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- W4220828122 cites W1547976855 @default.
- W4220828122 cites W1562015499 @default.
- W4220828122 cites W1590784449 @default.
- W4220828122 cites W1592902260 @default.
- W4220828122 cites W1965817092 @default.
- W4220828122 cites W1967420288 @default.
- W4220828122 cites W1970697372 @default.
- W4220828122 cites W1972010400 @default.
- W4220828122 cites W1976948973 @default.
- W4220828122 cites W1988422519 @default.
- W4220828122 cites W1992244851 @default.
- W4220828122 cites W1995673848 @default.
- W4220828122 cites W1997978648 @default.
- W4220828122 cites W2012195402 @default.
- W4220828122 cites W2012499122 @default.
- W4220828122 cites W2012731700 @default.
- W4220828122 cites W2014496529 @default.
- W4220828122 cites W2015022820 @default.
- W4220828122 cites W2016755746 @default.
- W4220828122 cites W2017188875 @default.
- W4220828122 cites W2018406411 @default.
- W4220828122 cites W2019651648 @default.
- W4220828122 cites W2031360652 @default.
- W4220828122 cites W2032738534 @default.
- W4220828122 cites W2038041834 @default.
- W4220828122 cites W2039220044 @default.
- W4220828122 cites W2040102816 @default.
- W4220828122 cites W2040233624 @default.
- W4220828122 cites W2051048419 @default.
- W4220828122 cites W2052696818 @default.
- W4220828122 cites W2053195173 @default.
- W4220828122 cites W2062276599 @default.
- W4220828122 cites W2065146040 @default.
- W4220828122 cites W2067169641 @default.
- W4220828122 cites W2073853609 @default.
- W4220828122 cites W2075611927 @default.
- W4220828122 cites W2076149193 @default.
- W4220828122 cites W2079415645 @default.
- W4220828122 cites W2081594548 @default.
- W4220828122 cites W2083346624 @default.
- W4220828122 cites W2097424323 @default.
- W4220828122 cites W2098555753 @default.
- W4220828122 cites W2110361765 @default.
- W4220828122 cites W2111137053 @default.
- W4220828122 cites W2121672428 @default.
- W4220828122 cites W2125312640 @default.
- W4220828122 cites W2134153749 @default.
- W4220828122 cites W2135105112 @default.
- W4220828122 cites W2136898237 @default.
- W4220828122 cites W2142752926 @default.
- W4220828122 cites W2146347752 @default.
- W4220828122 cites W2146375963 @default.
- W4220828122 cites W2152301430 @default.
- W4220828122 cites W2152786904 @default.
- W4220828122 cites W2160679365 @default.
- W4220828122 cites W2161256688 @default.
- W4220828122 cites W2165407655 @default.
- W4220828122 cites W2166215159 @default.
- W4220828122 cites W2170075812 @default.
- W4220828122 cites W2210686952 @default.
- W4220828122 cites W2257315280 @default.
- W4220828122 cites W2399010589 @default.
- W4220828122 cites W2521404215 @default.
- W4220828122 cites W2604756107 @default.
- W4220828122 cites W2726092399 @default.
- W4220828122 cites W2757528433 @default.
- W4220828122 cites W2782116549 @default.
- W4220828122 cites W2790129193 @default.
- W4220828122 cites W2796836297 @default.
- W4220828122 cites W2799627339 @default.
- W4220828122 cites W2883185578 @default.
- W4220828122 cites W2946448303 @default.
- W4220828122 cites W2977627461 @default.
- W4220828122 cites W2983037526 @default.
- W4220828122 cites W3037177471 @default.
- W4220828122 cites W3038403872 @default.
- W4220828122 cites W3039528361 @default.
- W4220828122 cites W3043701729 @default.
- W4220828122 cites W3117986865 @default.
- W4220828122 cites W3132554360 @default.
- W4220828122 cites W3162793164 @default.
- W4220828122 doi "https://doi.org/10.1007/s10482-022-01725-9" @default.
- W4220828122 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35322326" @default.
- W4220828122 hasPublicationYear "2022" @default.
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