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- W4220832885 abstract "Abstract Background: Selection of optimal computational strategies for analyzing metagenomics data is a decisive step in determining the microbial composition of a sample, and this procedure is complex because of the numerous tools currently available. The crowdsourced sbv IMPROVER Microbiomics Challenge and extended analyses benchmarked 21 off-the-shelf taxonomic metagenome profiling pipelines for their capacity to identify the microbiome composition at various taxon levels across 104 shotgun metagenomics datasets of bacterial genomes (representative of various microbiome samples) from public databases. Performance was determined by comparing predicted taxonomy profiles with the gold standard. Results: Most taxonomic profilers performed homogeneously well at the phylum level but generated intermediate and heterogeneous scores at the genus and species levels, respectively. kmer-based pipelines using Kraken with and without Bracken or using CLARK-S performed best overall, but they exhibited lower precision than the two marker-gene-based methods MetaPhlAn and mOTU. Filtering out the 1% least abundance species—which were not reliably predicted—helped increase the performance of most profilers by increasing precision but at the cost of recall. However, the use of adaptive filtering thresholds determined from the sample’s Shannon index increased the performance of most kmer-based profilers while mitigating the tradeoff between precision and recall. Conclusions: kmer-based metagenomic pipelines using Kraken/Bracken or CLARK-S performed most robustly across a large variety of microbiome datasets. Removing non-reliably predicted low-abundance species by using diversity-dependent adaptive filtering thresholds further enhanced the performance of these tools. This work demonstrates the applicability of computational pipelines for accurately determining taxonomic profiles in clinical and environmental contexts and exemplifies the power of crowdsourcing for unbiased evaluation." @default.
- W4220832885 created "2022-04-03" @default.
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- W4220832885 date "2022-03-31" @default.
- W4220832885 modified "2023-10-18" @default.
- W4220832885 title "Crowdsourced benchmarking of taxonomic metagenome profilers: Lessons learned from the sbv IMPROVER Microbiomics Challenge" @default.
- W4220832885 doi "https://doi.org/10.21203/rs.3.rs-1366315/v1" @default.
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