FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4220836485> ?p ?o ?g. }

• W4220836485 abstract "The genome of the hyperthermophilic archaeon Thermococcus onnurineus NA1 contains three copies of the formate dehydrogenase (FDH) gene, fdh1, fdh2, and fdh3. Previously, we reported that fdh2, clustered with genes encoding the multimeric membrane-bound hydrogenase and cation/proton antiporter, was essential for formate-dependent growth with H2 production. However, the functionality of the other two FDH-coding genes has not yet been elucidated. Herein, we purified and characterized cytoplasmic Fdh3 to understand its functionality. The purified Fdh3 was identified to be composed of a tungsten-containing catalytic subunit (Fdh3A), an NAD(P)-binding protein (Fdh3B), and two Fe-S proteins (Fdh3G1 and Fdh3G2). Fdh3 oxidized formate with specific activities of 241.7 U/mg and 77.4 U/mg using methyl viologen and NADP+ as electron acceptors, respectively. While most FDHs exhibited NAD+-dependent formate oxidation activity, the Fdh3 of T. onnurineus NA1 showed a strong preference for NADP+ over NAD+ as a cofactor. The catalytic efficiency (kcat/Km) of Fdh3 for NADP+ was measured to be 5,281 mM-1 s-1, which is the highest among NADP-dependent FDHs known to date. Structural modeling suggested that Arg204 and Arg205 of Fdh3B may contribute to the stabilization of the 2'-phosphate of NADP(H). Fdh3 could also use ferredoxin as an electron acceptor to oxidize formate with a specific activity of 0.83 U/mg. Furthermore, Fdh3 showed CO2 reduction activity using reduced ferredoxin or NADPH as an electron donor with a specific activity of 0.73 U/mg and 1.0 U/mg, respectively. These results suggest a functional role of Fdh3 in disposing of reducing equivalents by mediating electron transfer between formate and NAD(P)H or ferredoxin." @default.
• W4220836485 created "2022-04-03" @default.
• W4220836485 creator A5012238913 @default.
• W4220836485 creator A5042411782 @default.
• W4220836485 creator A5071649793 @default.
• W4220836485 creator A5073453218 @default.
• W4220836485 creator A5074507118 @default.
• W4220836485 date "2022-03-15" @default.
• W4220836485 modified "2023-09-25" @default.
• W4220836485 title "A Novel NADP-Dependent Formate Dehydrogenase From the Hyperthermophilic Archaeon Thermococcus onnurineus NA1" @default.
• W4220836485 cites W1156485060 @default.
• W4220836485 cites W1534024308 @default.
• W4220836485 cites W1605997234 @default.
• W4220836485 cites W1640038695 @default.
• W4220836485 cites W1670670197 @default.
• W4220836485 cites W1674876828 @default.
• W4220836485 cites W1877542297 @default.
• W4220836485 cites W1983996354 @default.
• W4220836485 cites W1985703657 @default.
• W4220836485 cites W1991450222 @default.
• W4220836485 cites W2014042585 @default.
• W4220836485 cites W2028692561 @default.
• W4220836485 cites W2032848096 @default.
• W4220836485 cites W2041952934 @default.
• W4220836485 cites W2055876759 @default.
• W4220836485 cites W2061788129 @default.
• W4220836485 cites W2064980217 @default.
• W4220836485 cites W2065790077 @default.
• W4220836485 cites W2067606391 @default.
• W4220836485 cites W2073049712 @default.
• W4220836485 cites W2079418187 @default.
• W4220836485 cites W2087647114 @default.
• W4220836485 cites W2093928137 @default.
• W4220836485 cites W2107495947 @default.
• W4220836485 cites W2112379603 @default.
• W4220836485 cites W2123024534 @default.
• W4220836485 cites W2128201660 @default.
• W4220836485 cites W2130415541 @default.
• W4220836485 cites W2142654170 @default.
• W4220836485 cites W2150032818 @default.
• W4220836485 cites W2151221082 @default.
• W4220836485 cites W2154687039 @default.
• W4220836485 cites W2159426340 @default.
• W4220836485 cites W2159868748 @default.
• W4220836485 cites W2172123465 @default.
• W4220836485 cites W2255482632 @default.
• W4220836485 cites W2290103494 @default.
• W4220836485 cites W2416231698 @default.
• W4220836485 cites W2465818893 @default.
• W4220836485 cites W2536171222 @default.
• W4220836485 cites W2589372192 @default.
• W4220836485 cites W2605619410 @default.
• W4220836485 cites W2614051244 @default.
• W4220836485 cites W2734553811 @default.
• W4220836485 cites W2804531237 @default.
• W4220836485 cites W2889499563 @default.
• W4220836485 cites W2920788159 @default.
• W4220836485 cites W2952249509 @default.
• W4220836485 cites W2955696126 @default.
• W4220836485 cites W3000124247 @default.
• W4220836485 cites W3017094108 @default.
• W4220836485 cites W3087487805 @default.
• W4220836485 cites W3115393581 @default.
• W4220836485 cites W3165317701 @default.
• W4220836485 cites W3165803424 @default.
• W4220836485 cites W3177828909 @default.
• W4220836485 cites W4210408855 @default.
• W4220836485 cites W4253395752 @default.
• W4220836485 doi "https://doi.org/10.3389/fmicb.2022.844735" @default.
• W4220836485 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35369452" @default.
• W4220836485 hasPublicationYear "2022" @default.
• W4220836485 type Work @default.
• W4220836485 citedByCount "3" @default.
• W4220836485 countsByYear W42208364852023 @default.
• W4220836485 crossrefType "journal-article" @default.
• W4220836485 hasAuthorship W4220836485A5012238913 @default.
• W4220836485 hasAuthorship W4220836485A5042411782 @default.
• W4220836485 hasAuthorship W4220836485A5071649793 @default.
• W4220836485 hasAuthorship W4220836485A5073453218 @default.
• W4220836485 hasAuthorship W4220836485A5074507118 @default.
• W4220836485 hasBestOaLocation W42208364851 @default.
• W4220836485 hasConcept C104292427 @default.
• W4220836485 hasConcept C104317684 @default.
• W4220836485 hasConcept C134621786 @default.
• W4220836485 hasConcept C161790260 @default.
• W4220836485 hasConcept C181199279 @default.
• W4220836485 hasConcept C185592680 @default.
• W4220836485 hasConcept C197957613 @default.
• W4220836485 hasConcept C2776943354 @default.
• W4220836485 hasConcept C2778428456 @default.
• W4220836485 hasConcept C2778899470 @default.
• W4220836485 hasConcept C2779268744 @default.
• W4220836485 hasConcept C2780178953 @default.
• W4220836485 hasConcept C550995028 @default.
• W4220836485 hasConcept C55428944 @default.
• W4220836485 hasConcept C55493867 @default.
• W4220836485 hasConcept C71240020 @default.
• W4220836485 hasConcept C75520062 @default.
• W4220836485 hasConcept C79115784 @default.
• W4220836485 hasConcept C86803240 @default.

• next