FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4220845837> ?p ?o ?g. }

• W4220845837 endingPage "95" @default.
• W4220845837 startingPage "81" @default.
• W4220845837 abstract "Ulcerative colitis (UC) is one type of inflammatory bowel disease (IBD) and lactoferrin (LF) is a promising protein drug to treat UC. However, targeted LF delivery to optimize bioavailability, targeting and effectiveness remains a challenge. Here, we report an effective strategy to fabricate silk sericin nanospheres systems for the delivery of recombinant human lactoferrin (SS-NS-rhLF). The system is based on the use of optimized transgenic silkworms to generate genetically engineered silk fibers (rhLF-silks). The rhLF silks were used for fabricating SS-NS-rhLF by ethanol precipitation. The SS-NS-rhLF were stable with a spherical morphology with an average diameter of 123 nm. The negatively charged sericins in a pH ≥ 5.5 environment achieved specific targeting of the SS-NS-rhLF to positively charged colonic sites. The SS-NS-rhLF achieved efficient uptake by cells in the inflamed colon of mice when compared to free lactoferrin in solution (SOL-rhLF). Furthermore, oral administration of the SS-NS-rhLF with low dose of rhLF significantly relived symptoms of UC in mice and achieved comparable therapeutic effect to the high dose of SOL-rhLF by supporting the reformation of cell structure and length of colon tissue, reducing the release of inflammatory factors, inhibiting the activation of the NF-κB inflammatory pathway, and maintaining a stable intestinal microbial population in mice. These results showed that the SS-NS-rhLF is a promising system for colitis treatment. STATEMENT OF SIGNIFICANCE: Targeting and effective delivery of multiple biological functional protein human lactoferrin (rhLF) is a promising strategy to treat ulcerative colitis in the clinic. Here, rhLF-transgenic silk cocoons were used to fabricate a rhLF-sericin nanosphere delivery system (SS-NS-rhLF). The fabricated SS-NS-rhLF showed identical spherical morphology, stable structure, sustainable rhLF release, efficient cell uptake and negative charge in an environment of pH above 5.5, thus realized the specific targeting to the positively charged colonic sites to treat UC mice through oral administration. The therapeutic effect of SS-NS-rhLF with a low rhLF dose in the UC mice was comparable to the high dose of free rhLF treatment in solution form, suggesting that the SS-NS-rhLF is a promising system for colitis treatment." @default.
• W4220845837 created "2022-04-03" @default.
• W4220845837 creator A5005788616 @default.
• W4220845837 creator A5036911243 @default.
• W4220845837 creator A5040950146 @default.
• W4220845837 creator A5041326706 @default.
• W4220845837 creator A5057194915 @default.
• W4220845837 creator A5057871048 @default.
• W4220845837 creator A5058927911 @default.
• W4220845837 creator A5061369541 @default.
• W4220845837 creator A5065906949 @default.
• W4220845837 creator A5086164451 @default.
• W4220845837 date "2022-05-01" @default.
• W4220845837 modified "2023-10-16" @default.
• W4220845837 title "Genetically engineered pH-responsive silk sericin nanospheres with efficient therapeutic effect on ulcerative colitis" @default.
• W4220845837 cites W1444793581 @default.
• W4220845837 cites W1683971004 @default.
• W4220845837 cites W1752299999 @default.
• W4220845837 cites W1937091029 @default.
• W4220845837 cites W1985716931 @default.
• W4220845837 cites W2000409833 @default.
• W4220845837 cites W2012149997 @default.
• W4220845837 cites W2033689464 @default.
• W4220845837 cites W2051158064 @default.
• W4220845837 cites W2057333689 @default.
• W4220845837 cites W2071649876 @default.
• W4220845837 cites W2081024145 @default.
• W4220845837 cites W2082500130 @default.
• W4220845837 cites W2088170171 @default.
• W4220845837 cites W2092481385 @default.
• W4220845837 cites W2094577650 @default.
• W4220845837 cites W2116846792 @default.
• W4220845837 cites W2133547370 @default.
• W4220845837 cites W2149170290 @default.
• W4220845837 cites W2151798187 @default.
• W4220845837 cites W2154967631 @default.
• W4220845837 cites W2165649546 @default.
• W4220845837 cites W2290667492 @default.
• W4220845837 cites W2339102023 @default.
• W4220845837 cites W2407300501 @default.
• W4220845837 cites W2414266001 @default.
• W4220845837 cites W2514215197 @default.
• W4220845837 cites W2553157303 @default.
• W4220845837 cites W2611600531 @default.
• W4220845837 cites W2620756707 @default.
• W4220845837 cites W2724938313 @default.
• W4220845837 cites W2732117961 @default.
• W4220845837 cites W2756118428 @default.
• W4220845837 cites W2807594408 @default.
• W4220845837 cites W2873908887 @default.
• W4220845837 cites W2888196681 @default.
• W4220845837 cites W2889211833 @default.
• W4220845837 cites W2891767471 @default.
• W4220845837 cites W2906024285 @default.
• W4220845837 cites W2913284233 @default.
• W4220845837 cites W2944726119 @default.
• W4220845837 cites W2949944282 @default.
• W4220845837 cites W2956149594 @default.
• W4220845837 cites W2971706394 @default.
• W4220845837 cites W2979788235 @default.
• W4220845837 cites W2987309981 @default.
• W4220845837 cites W2987419736 @default.
• W4220845837 cites W2994506563 @default.
• W4220845837 cites W2997898663 @default.
• W4220845837 cites W3001866303 @default.
• W4220845837 cites W3011661918 @default.
• W4220845837 cites W3021241071 @default.
• W4220845837 cites W3036502373 @default.
• W4220845837 cites W3048836615 @default.
• W4220845837 cites W3055242725 @default.
• W4220845837 cites W3081542391 @default.
• W4220845837 cites W3105071922 @default.
• W4220845837 cites W3107767555 @default.
• W4220845837 cites W3111012652 @default.
• W4220845837 cites W3125204155 @default.
• W4220845837 cites W3126436367 @default.
• W4220845837 cites W3150733764 @default.
• W4220845837 cites W3199527627 @default.
• W4220845837 doi "https://doi.org/10.1016/j.actbio.2022.03.012" @default.
• W4220845837 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35288310" @default.
• W4220845837 hasPublicationYear "2022" @default.
• W4220845837 type Work @default.
• W4220845837 citedByCount "21" @default.
• W4220845837 countsByYear W42208458372022 @default.
• W4220845837 countsByYear W42208458372023 @default.
• W4220845837 crossrefType "journal-article" @default.
• W4220845837 hasAuthorship W4220845837A5005788616 @default.
• W4220845837 hasAuthorship W4220845837A5036911243 @default.
• W4220845837 hasAuthorship W4220845837A5040950146 @default.
• W4220845837 hasAuthorship W4220845837A5041326706 @default.
• W4220845837 hasAuthorship W4220845837A5057194915 @default.
• W4220845837 hasAuthorship W4220845837A5057871048 @default.
• W4220845837 hasAuthorship W4220845837A5058927911 @default.
• W4220845837 hasAuthorship W4220845837A5061369541 @default.
• W4220845837 hasAuthorship W4220845837A5065906949 @default.
• W4220845837 hasAuthorship W4220845837A5086164451 @default.
• W4220845837 hasConcept C122302759 @default.
• W4220845837 hasConcept C142724271 @default.

• next