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• W4220851471 endingPage "108989" @default.
• W4220851471 startingPage "108989" @default.
• W4220851471 abstract "Adulteration of halal-certified food with pork are often reported. Using direct PCR results in cost- and time-saving benefits, and allows flexibility in amplicon detection. Here, we aimed to develop and validate a novel, direct asymmetric PCR and catalyzed hairpin assembly fluorescent biosensor (daPCR-CHA-FB) with a portable LED lightbox for naked eye detection of porcine DNA in raw and processed food. We tested the assay's reproducibility, specificity, sensitivity, real-world applicability, and single-blind performance. Using only 1 mm3 samples, reproducibility test with 38 raw pork samples showed 2.6% false negative rate and 0.0% false positive rate. The lowest detection limits were 0.01 ng for porcine DNA and 0.1% for binary pork-and-beef mixture. No cross-reaction with 16 other species was observed. One-hundred market samples showed no false negative for pork-listed samples, while two samples declared as non-pork showed a positive reaction. The assay was simple, sensitive, specific, safe, and robust. It could be used by law enforcement agencies and small-scale laboratories to investigate contamination and adulteration of halal foods." @default.
• W4220851471 created "2022-04-03" @default.
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• W4220851471 date "2022-09-01" @default.
• W4220851471 modified "2023-09-30" @default.
• W4220851471 title "Detection of porcine DNA in food using direct asymmetric PCR and catalyzed hairpin assembly fluorescent biosensor: A novel assay for halal food analysis" @default.
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• W4220851471 doi "https://doi.org/10.1016/j.foodcont.2022.108989" @default.
• W4220851471 hasPublicationYear "2022" @default.
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