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• W4220853511 endingPage "e0265270" @default.
• W4220853511 startingPage "e0265270" @default.
• W4220853511 abstract "Wheat is an important cereal and half of the world population consumed it. Wheat faces environmental stresses and different techniques (CRISPR, gene silencing, GWAS, etc.) were used to enhance its production but RNA editing (RESs) is not fully explored in wheat. RNA editing has a special role in controlling environmental stresses. The genome-wide identification and functional characterization of RESs in different types of wheat genotypes was done. We employed six wheat genotypes by RNA-seq analyses to achieve RESs. The findings revealed that RNA editing events occurred on all chromosomes equally. RNA editing sites were distributed randomly and 10–12 types of RESs were detected in wheat genotypes. Higher number of RESs were detected in drought-tolerant genotypes. A-to-I RNA editing (2952, 2977, 1916, 2576, 3422, and 3459) sites were also identified in six wheat genotypes. Most of the genes were found to be engaged in molecular processes after a Gene Ontology analysis. PPR ( pentatricopeptide repeat ), OZ1 ( organelle zinc-finger ), and MORF/RIP gene expression levels in wheat were also examined. Normal growth conditions diverge gene expression of these three different gene families, implying that normal growth conditions for various genotypes can modify RNA editing events and have an impact on gene expression levels. While the expression of PPR genes was not change. We used Variant Effect Predictor (VEP) to annotate RNA editing sites, and Local White had the highest RESs in the CDS region of the protein. These findings will be useful for prediction of RESs in other crops and will be helpful in drought tolerance development in wheat." @default.
• W4220853511 created "2022-04-03" @default.
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• W4220853511 date "2022-03-11" @default.
• W4220853511 modified "2023-10-18" @default.
• W4220853511 title "Genome-wide investigation and functional analysis of RNA editing sites in wheat" @default.
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• W4220853511 doi "https://doi.org/10.1371/journal.pone.0265270" @default.
• W4220853511 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35275970" @default.
• W4220853511 hasPublicationYear "2022" @default.
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