FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4220854387> ?p ?o ?g. }

• W4220854387 endingPage "101965" @default.
• W4220854387 startingPage "101965" @default.
• W4220854387 abstract "The COVID-19 was identified for the first time from the sea food market, Wuhan city, China in 2019 and the pathogenic organism was identified as SARS-CoV-2. Currently, this virus has spread to 223 countries and territories and known as a serious issue for the global human community. Many vaccines have been developed and used for immunization.We have reported the insilico prediction, designing, secondary structure prediction, molecular docking analysis, and in vitro assessment of siRNAs against SARS-CoV-2. The online bioinformatic approach was used for siRNAs selection and designing. The selected siRNAs were evaluated for antiviral efficacy by using Lipofectamine 2000 as delivery agent to HEK-293 cells. The MTT assay was used for cytotoxicity determination. The antiviral efficacy of potential siRNAs was determined based on the Ct value of q-RT-PCR and the data analysis was done by Prism-GraphPad software.The analyzed data resulted in the selection of only three siRNAs out of twenty-six siRNAs generated by online software. The secondary structure prediction and molecular docking analysis of siRNAs revealed the efficient binding to the target. There was no cellular toxicity observed in the HEK-293 cells at any tested concentrations of siRNAs. The purification of RNA was completed from inoculated cells and subjected to q-RT-PCR. The highest Ct value was observed in siRNA 3 than the others. The results offered valuable evidence and invigorated us to assess the potency of siRNAs by using alone or in combination in other human cells.The data generated from this study indicates the significance of in silico prediction and narrow down the potential siRNA' against SARS-CoV-2, and molecular docking investigation offered the effective siRNAs binding with the target. Finally, it is concluded that the online bioinformatics approach provided the prediction and selection of siRNAs with better antiviral efficacy. The siRNA-3 was observed to be the best for reduction of viral RNA in cells." @default.
• W4220854387 created "2022-04-03" @default.
• W4220854387 creator A5030781607 @default.
• W4220854387 creator A5060318980 @default.
• W4220854387 creator A5080738391 @default.
• W4220854387 date "2022-06-01" @default.
• W4220854387 modified "2023-10-01" @default.
• W4220854387 title "Effect of insilico predicted and designed potential siRNAs on inhibition of SARS-CoV-2 in HEK-293 cells" @default.
• W4220854387 cites W2029938306 @default.
• W4220854387 cites W2037269568 @default.
• W4220854387 cites W2039746015 @default.
• W4220854387 cites W2066944164 @default.
• W4220854387 cites W2073858684 @default.
• W4220854387 cites W2148419405 @default.
• W4220854387 cites W2624827575 @default.
• W4220854387 cites W2767666716 @default.
• W4220854387 cites W2946725940 @default.
• W4220854387 cites W3014298809 @default.
• W4220854387 cites W3015389434 @default.
• W4220854387 cites W3046658973 @default.
• W4220854387 cites W3048733387 @default.
• W4220854387 cites W3082059289 @default.
• W4220854387 cites W3107708955 @default.
• W4220854387 cites W3111184403 @default.
• W4220854387 cites W3113735753 @default.
• W4220854387 cites W3120614536 @default.
• W4220854387 cites W3130817332 @default.
• W4220854387 cites W3133474297 @default.
• W4220854387 cites W3135502188 @default.
• W4220854387 cites W3153569168 @default.
• W4220854387 cites W3159555134 @default.
• W4220854387 cites W3159860948 @default.
• W4220854387 cites W3160304872 @default.
• W4220854387 cites W3161563929 @default.
• W4220854387 cites W3165479483 @default.
• W4220854387 cites W3172780880 @default.
• W4220854387 cites W3179071715 @default.
• W4220854387 cites W3179206042 @default.
• W4220854387 cites W3179259355 @default.
• W4220854387 cites W3195944396 @default.
• W4220854387 cites W3203870163 @default.
• W4220854387 cites W4205801190 @default.
• W4220854387 cites W3106704356 @default.
• W4220854387 doi "https://doi.org/10.1016/j.jksus.2022.101965" @default.
• W4220854387 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35313445" @default.
• W4220854387 hasPublicationYear "2022" @default.
• W4220854387 type Work @default.
• W4220854387 citedByCount "3" @default.
• W4220854387 countsByYear W42208543872022 @default.
• W4220854387 countsByYear W42208543872023 @default.
• W4220854387 crossrefType "journal-article" @default.
• W4220854387 hasAuthorship W4220854387A5030781607 @default.
• W4220854387 hasAuthorship W4220854387A5060318980 @default.
• W4220854387 hasAuthorship W4220854387A5080738391 @default.
• W4220854387 hasBestOaLocation W42208543871 @default.
• W4220854387 hasConcept C104317684 @default.
• W4220854387 hasConcept C109316439 @default.
• W4220854387 hasConcept C159047783 @default.
• W4220854387 hasConcept C166703698 @default.
• W4220854387 hasConcept C174510640 @default.
• W4220854387 hasConcept C202751555 @default.
• W4220854387 hasConcept C22615655 @default.
• W4220854387 hasConcept C2522874641 @default.
• W4220854387 hasConcept C40767141 @default.
• W4220854387 hasConcept C49923114 @default.
• W4220854387 hasConcept C54355233 @default.
• W4220854387 hasConcept C55493867 @default.
• W4220854387 hasConcept C67705224 @default.
• W4220854387 hasConcept C70721500 @default.
• W4220854387 hasConcept C71924100 @default.
• W4220854387 hasConcept C81885089 @default.
• W4220854387 hasConcept C86803240 @default.
• W4220854387 hasConcept C92087593 @default.
• W4220854387 hasConcept C98274493 @default.
• W4220854387 hasConceptScore W4220854387C104317684 @default.
• W4220854387 hasConceptScore W4220854387C109316439 @default.
• W4220854387 hasConceptScore W4220854387C159047783 @default.
• W4220854387 hasConceptScore W4220854387C166703698 @default.
• W4220854387 hasConceptScore W4220854387C174510640 @default.
• W4220854387 hasConceptScore W4220854387C202751555 @default.
• W4220854387 hasConceptScore W4220854387C22615655 @default.
• W4220854387 hasConceptScore W4220854387C2522874641 @default.
• W4220854387 hasConceptScore W4220854387C40767141 @default.
• W4220854387 hasConceptScore W4220854387C49923114 @default.
• W4220854387 hasConceptScore W4220854387C54355233 @default.
• W4220854387 hasConceptScore W4220854387C55493867 @default.
• W4220854387 hasConceptScore W4220854387C67705224 @default.
• W4220854387 hasConceptScore W4220854387C70721500 @default.
• W4220854387 hasConceptScore W4220854387C71924100 @default.
• W4220854387 hasConceptScore W4220854387C81885089 @default.
• W4220854387 hasConceptScore W4220854387C86803240 @default.
• W4220854387 hasConceptScore W4220854387C92087593 @default.
• W4220854387 hasConceptScore W4220854387C98274493 @default.
• W4220854387 hasIssue "4" @default.
• W4220854387 hasLocation W42208543871 @default.
• W4220854387 hasLocation W42208543872 @default.
• W4220854387 hasLocation W42208543873 @default.
• W4220854387 hasOpenAccess W4220854387 @default.

• next