FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4220867900> ?p ?o ?g. }

• W4220867900 endingPage "e1010355" @default.
• W4220867900 startingPage "e1010355" @default.
• W4220867900 abstract "Human cytomegalovirus (HCMV) is a major pathogen in immunocompromised patients. The UL146 gene exists as 14 diverse genotypes among clinical isolates, which encode 14 different CXC chemokines. One genotype (vCXCL1 GT1 ) is a known agonist for CXCR1 and CXCR2, while two others (vCXCL1 GT5 and vCXCL1 GT6 ) lack the ELR motif considered crucial for CXCR1 and CXCR2 binding, thus suggesting another receptor targeting profile. To determine the receptor target for vCXCL1 GT5 , the chemokine was probed in a G protein signaling assay on all 18 classical human chemokine receptors, where CXCR2 was the only receptor being activated. In addition, vCXCL1 GT5 recruited β-arrestin in a BRET-based assay and induced migration in a chemotaxis assay through CXCR2, but not CXCR1. In contrast, vCXCL1 GT1 stimulated G protein signaling, recruited β-arrestin and induced migration through both CXCR1 and CXCR2. Both vCXCL1 GT1 and vCXCL1 GT5 induced equally potent and efficacious migration of neutrophils, and ELR vCXCL1 GT4 and non-ELR vCXCL1 GT6 activated only CXCR2. In contrast to most human chemokines, the 14 UL146 genotypes have remarkably long C-termini. Comparative modeling using Rosetta showed that each genotype could adopt the classic chemokine core structure, and predicted that the extended C-terminal tail of several genotypes (including vCXCL1 GT1 , vCXCL1 GT4 , vCXCL1 GT5 , and vCXCL1 GT6 ) forms a novel β-hairpin not found in human chemokines. Secondary NMR shift and TALOS+ analysis of vCXCL1 GT1 supported the existence of two stable β-strands. C-terminal deletion of vCXCL1 GT1 resulted in a non-functional protein and in a shift to solvent exposure for tryptophan residues likely due to destabilization of the chemokine fold. The results demonstrate that non-ELR chemokines can activate CXCR2 and suggest that the UL146 chemokines have unique C-terminal structures that stabilize the chemokine fold. Increased knowledge of the structure and interaction partners of the chemokine variants encoded by UL146 is key to understanding why circulating HCMV strains sustain 14 stable genotypes." @default.
• W4220867900 created "2022-04-03" @default.
• W4220867900 creator A5007251119 @default.
• W4220867900 creator A5007750328 @default.
• W4220867900 creator A5029709016 @default.
• W4220867900 creator A5033914407 @default.
• W4220867900 creator A5035872776 @default.
• W4220867900 creator A5038306596 @default.
• W4220867900 creator A5079028211 @default.
• W4220867900 creator A5082430232 @default.
• W4220867900 creator A5088428038 @default.
• W4220867900 creator A5088703972 @default.
• W4220867900 date "2022-03-10" @default.
• W4220867900 modified "2023-10-14" @default.
• W4220867900 title "The non-ELR CXC chemokine encoded by human cytomegalovirus UL146 genotype 5 contains a C-terminal β-hairpin and induces neutrophil migration as a selective CXCR2 agonist" @default.
• W4220867900 cites W1490744061 @default.
• W4220867900 cites W1517124487 @default.
• W4220867900 cites W1529537962 @default.
• W4220867900 cites W1579558830 @default.
• W4220867900 cites W1614726407 @default.
• W4220867900 cites W1616296090 @default.
• W4220867900 cites W1877691250 @default.
• W4220867900 cites W1922705469 @default.
• W4220867900 cites W1968927848 @default.
• W4220867900 cites W1979964053 @default.
• W4220867900 cites W1983086155 @default.
• W4220867900 cites W1986322598 @default.
• W4220867900 cites W1990601982 @default.
• W4220867900 cites W1995055452 @default.
• W4220867900 cites W2000948557 @default.
• W4220867900 cites W2007070916 @default.
• W4220867900 cites W2007823692 @default.
• W4220867900 cites W2012730930 @default.
• W4220867900 cites W2019607838 @default.
• W4220867900 cites W2030533119 @default.
• W4220867900 cites W2030569505 @default.
• W4220867900 cites W2032612463 @default.
• W4220867900 cites W2042927644 @default.
• W4220867900 cites W2043042627 @default.
• W4220867900 cites W2046825058 @default.
• W4220867900 cites W2053031231 @default.
• W4220867900 cites W2053110044 @default.
• W4220867900 cites W2053902929 @default.
• W4220867900 cites W2057350048 @default.
• W4220867900 cites W2059845506 @default.
• W4220867900 cites W2062521099 @default.
• W4220867900 cites W2062923301 @default.
• W4220867900 cites W2072918899 @default.
• W4220867900 cites W2090693744 @default.
• W4220867900 cites W2101791639 @default.
• W4220867900 cites W2103868650 @default.
• W4220867900 cites W2104316132 @default.
• W4220867900 cites W2112418305 @default.
• W4220867900 cites W2119261083 @default.
• W4220867900 cites W2123318089 @default.
• W4220867900 cites W2123731815 @default.
• W4220867900 cites W2125159941 @default.
• W4220867900 cites W2130397839 @default.
• W4220867900 cites W2132411790 @default.
• W4220867900 cites W2133451598 @default.
• W4220867900 cites W2141401084 @default.
• W4220867900 cites W2144240179 @default.
• W4220867900 cites W2146193551 @default.
• W4220867900 cites W2152381037 @default.
• W4220867900 cites W2168036974 @default.
• W4220867900 cites W2169174459 @default.
• W4220867900 cites W2169821755 @default.
• W4220867900 cites W2273967916 @default.
• W4220867900 cites W2346822635 @default.
• W4220867900 cites W2463387538 @default.
• W4220867900 cites W2519406656 @default.
• W4220867900 cites W2560324708 @default.
• W4220867900 cites W2609207099 @default.
• W4220867900 cites W2768244904 @default.
• W4220867900 cites W2808393899 @default.
• W4220867900 cites W2888246207 @default.
• W4220867900 cites W2904881174 @default.
• W4220867900 cites W2950963668 @default.
• W4220867900 cites W2956138021 @default.
• W4220867900 cites W3012311885 @default.
• W4220867900 cites W3038311311 @default.
• W4220867900 cites W3128367803 @default.
• W4220867900 cites W3158330558 @default.
• W4220867900 cites W72907974 @default.
• W4220867900 cites W2806418244 @default.
• W4220867900 doi "https://doi.org/10.1371/journal.ppat.1010355" @default.
• W4220867900 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35271688" @default.
• W4220867900 hasPublicationYear "2022" @default.
• W4220867900 type Work @default.
• W4220867900 citedByCount "2" @default.
• W4220867900 countsByYear W42208679002023 @default.
• W4220867900 crossrefType "journal-article" @default.
• W4220867900 hasAuthorship W4220867900A5007251119 @default.
• W4220867900 hasAuthorship W4220867900A5007750328 @default.
• W4220867900 hasAuthorship W4220867900A5029709016 @default.
• W4220867900 hasAuthorship W4220867900A5033914407 @default.
• W4220867900 hasAuthorship W4220867900A5035872776 @default.
• W4220867900 hasAuthorship W4220867900A5038306596 @default.

• next