FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4220872014> ?p ?o ?g. }

• W4220872014 abstract "&lt;p&gt;A better understanding of soil carbon sequestration is important as the rapid elevation of carbon emission has significantly impacted our climate. The soil carbon dynamic process is strongly associated with trophic interactions in the soil communities. However, direct investigation of microbes and trophic interactions at microscale has been a major challenge due to the opacity of soil and the exceedingly complex pore spaces that limit direct in situ observation of trophic interactions and their variations with soil structure. As such, we decide to use microfluidic technique to create a two dimensional heterogeneous porous microenvironment containing nutrient patches of different quantity and quality to mimic the complex soil pore network and its heterogenous distribution of resources. This allows us to directly visualize and investigate the ability of organisms to access spaces starting from an initial ecophysiological precondition to changes of spatial accessibility mediated by interactions with the microbial community.&lt;/p&gt;&lt;p&gt;Microfluidics is a multidisciplinary platform that integrates micro fabrication, physical chemistry analysis, automation and microscopy. It has been widely used in life science and chemistry as it allows precise liquid manipulation, rapid measurements, real-time visualization at microscale, which is especially of interest and benefit to microbial studies. We created a complex pore network mimicking different soil environments &amp;#8211; earlier considered impossible to achieve experimentally. The microfluidic channel contains a random distribution of cylindrical pillars of different sizes so as to mimic the pore space variations found in real soil. The randomness in the design creates various spatial availability for microbes. The nutrient patches within the pore space are achieved via capillary force trapping or UV curing hydrogel. In the former method, the patches are created by trapping nutrients in the predesign pore space due to interfacial tension between air and nutrient liquid. However, displacement instability during air injection makes it hard to control the formation of the patches. The latter enables better and more precise patches size and location manipulation but hydrogel biocompatibility with various fungal species remains a major challenge and hydrogel curing requires special facilities. The microfluidic nutrient patches provide spatial heterogeneities of resource distribution, which allows us to have a better understanding of the influence of spatial accessibility on the microbial community. The chip has five different nutrient distribution levels, ranging from centralized &amp;#8211; a single large nutrient patch- &amp;#160;to increasingly dispersed nutrient distributions - sets of up to 49 loosely distributed nutrient patches of equal total volume. The experiments will be carried out using sterile cultures of fluorescent bacteria and fungi, synthetic communities of combinations of these, or a whole soil community inoculum. We will quantify the consumption of organic matter from the different areas via fluorescent substrates, and measure the bio- and necromass produced. We hypothesise that denser distribution will increase the net decomposition of organic matter as a centralized nutrient location increases the interactions within the microbial communities and individuals present in that area, which induces higher competition stress in the different communities.&lt;/p&gt;" @default.
• W4220872014 created "2022-04-03" @default.
• W4220872014 creator A5050207871 @default.
• W4220872014 creator A5056385602 @default.
• W4220872014 creator A5063621051 @default.
• W4220872014 date "2022-03-28" @default.
• W4220872014 modified "2023-09-29" @default.
• W4220872014 title "Two-dimensional microfluidic nutrient patches for direct visualization of microbial resource cycling&#160;" @default.
• W4220872014 doi "https://doi.org/10.5194/egusphere-egu22-9836" @default.
• W4220872014 hasPublicationYear "2022" @default.
• W4220872014 type Work @default.
• W4220872014 citedByCount "0" @default.
• W4220872014 crossrefType "posted-content" @default.
• W4220872014 hasAuthorship W4220872014A5050207871 @default.
• W4220872014 hasAuthorship W4220872014A5056385602 @default.
• W4220872014 hasAuthorship W4220872014A5063621051 @default.
• W4220872014 hasConcept C142796444 @default.
• W4220872014 hasConcept C145420912 @default.
• W4220872014 hasConcept C159390177 @default.
• W4220872014 hasConcept C159750122 @default.
• W4220872014 hasConcept C159985019 @default.
• W4220872014 hasConcept C161790260 @default.
• W4220872014 hasConcept C164638232 @default.
• W4220872014 hasConcept C171250308 @default.
• W4220872014 hasConcept C179428855 @default.
• W4220872014 hasConcept C185592680 @default.
• W4220872014 hasConcept C186060115 @default.
• W4220872014 hasConcept C18903297 @default.
• W4220872014 hasConcept C192562407 @default.
• W4220872014 hasConcept C33923547 @default.
• W4220872014 hasConcept C39432304 @default.
• W4220872014 hasConcept C55493867 @default.
• W4220872014 hasConcept C57924286 @default.
• W4220872014 hasConcept C6648577 @default.
• W4220872014 hasConcept C72958200 @default.
• W4220872014 hasConcept C78609370 @default.
• W4220872014 hasConcept C82776694 @default.
• W4220872014 hasConcept C8673954 @default.
• W4220872014 hasConcept C86803240 @default.
• W4220872014 hasConceptScore W4220872014C142796444 @default.
• W4220872014 hasConceptScore W4220872014C145420912 @default.
• W4220872014 hasConceptScore W4220872014C159390177 @default.
• W4220872014 hasConceptScore W4220872014C159750122 @default.
• W4220872014 hasConceptScore W4220872014C159985019 @default.
• W4220872014 hasConceptScore W4220872014C161790260 @default.
• W4220872014 hasConceptScore W4220872014C164638232 @default.
• W4220872014 hasConceptScore W4220872014C171250308 @default.
• W4220872014 hasConceptScore W4220872014C179428855 @default.
• W4220872014 hasConceptScore W4220872014C185592680 @default.
• W4220872014 hasConceptScore W4220872014C186060115 @default.
• W4220872014 hasConceptScore W4220872014C18903297 @default.
• W4220872014 hasConceptScore W4220872014C192562407 @default.
• W4220872014 hasConceptScore W4220872014C33923547 @default.
• W4220872014 hasConceptScore W4220872014C39432304 @default.
• W4220872014 hasConceptScore W4220872014C55493867 @default.
• W4220872014 hasConceptScore W4220872014C57924286 @default.
• W4220872014 hasConceptScore W4220872014C6648577 @default.
• W4220872014 hasConceptScore W4220872014C72958200 @default.
• W4220872014 hasConceptScore W4220872014C78609370 @default.
• W4220872014 hasConceptScore W4220872014C82776694 @default.
• W4220872014 hasConceptScore W4220872014C8673954 @default.
• W4220872014 hasConceptScore W4220872014C86803240 @default.
• W4220872014 hasLocation W42208720141 @default.
• W4220872014 hasOpenAccess W4220872014 @default.
• W4220872014 hasPrimaryLocation W42208720141 @default.
• W4220872014 hasRelatedWork W1995745284 @default.
• W4220872014 hasRelatedWork W2034171499 @default.
• W4220872014 hasRelatedWork W2044068276 @default.
• W4220872014 hasRelatedWork W2046567759 @default.
• W4220872014 hasRelatedWork W2095421566 @default.
• W4220872014 hasRelatedWork W2152341986 @default.
• W4220872014 hasRelatedWork W23078202 @default.
• W4220872014 hasRelatedWork W4200507042 @default.
• W4220872014 hasRelatedWork W4220872014 @default.
• W4220872014 hasRelatedWork W4312100075 @default.
• W4220872014 isParatext "false" @default.
• W4220872014 isRetracted "false" @default.
• W4220872014 workType "article" @default.