Matches in SemOpenAlex for { <https://semopenalex.org/work/W4220876852> ?p ?o ?g. }
- W4220876852 abstract "Bifidobacteria are gram-positive, probiotic, and generally regarded as safe bacteria. Techniques such as transformation, gene knockout, and heterologous gene expression have been established for Bifidobacterium, indicating that this bacterium can be used as a cell factory platform. However, there are limited previous reports in this field, likely because of factors such as the highly anaerobic nature of this bacterium. Bifidobacterium adolescentis is among the most oxygen-sensitive Bifidobacterium species. It shows strain-specific gamma-aminobutyric acid (GABA) production. GABA is a potent bioactive compound with numerous physiological and psychological functions. In this study, we investigated whether B. adolesentis could be used for mass production of GABA.The B. adolescentis 4-2 strain isolated from a healthy adult human produced approximately 14 mM GABA. It carried gadB and gadC, which encode glutamate decarboxylase and glutamate GABA antiporter, respectively. We constructed pKKT427::Pori-gadBC and pKKT427::Pgap-gadBC plasmids carrying gadBC driven by the original gadB (ori) and gap promoters, respectively. Recombinants of Bifidobacterium were then constructed. Two recombinants with high production abilities, monitored by two different promoters, were investigated. GABA production was improved by adjusting the fermentation parameters, including the substrate concentration, initial culture pH, and co-factor supplementation, using response surface methodology. The optimum initial cultivation pH varied when the promoter region was changed. The ori promoter was induced under acidic conditions (pH 5.2:4.4), whereas the constitutive gap promoter showed enhanced GABA production at pH 6.0. Fed-batch fermentation was used to validate the optimum fermentation parameters, in which approximately 415 mM GABA was produced. The conversion ratio of glutamate to GABA was 92-100%.We report high GABA production in recombinant B. adolescentis. This study provides a foundation for using Bifidobacterium as a cell factory platform for industrial production of GABA." @default.
- W4220876852 created "2022-04-03" @default.
- W4220876852 creator A5014358648 @default.
- W4220876852 creator A5052829635 @default.
- W4220876852 creator A5061817773 @default.
- W4220876852 creator A5067666867 @default.
- W4220876852 creator A5075747124 @default.
- W4220876852 creator A5085236029 @default.
- W4220876852 creator A5086511480 @default.
- W4220876852 creator A5087006756 @default.
- W4220876852 date "2022-03-07" @default.
- W4220876852 modified "2023-10-15" @default.
- W4220876852 title "Cell factory for γ-aminobutyric acid (GABA) production using Bifidobacterium adolescentis" @default.
- W4220876852 cites W178872016 @default.
- W4220876852 cites W1963946970 @default.
- W4220876852 cites W1964841183 @default.
- W4220876852 cites W1967153009 @default.
- W4220876852 cites W1983572173 @default.
- W4220876852 cites W1987395465 @default.
- W4220876852 cites W1990484977 @default.
- W4220876852 cites W2016667239 @default.
- W4220876852 cites W2021762085 @default.
- W4220876852 cites W2022966294 @default.
- W4220876852 cites W2027216673 @default.
- W4220876852 cites W2033896405 @default.
- W4220876852 cites W2047001261 @default.
- W4220876852 cites W2047601444 @default.
- W4220876852 cites W2057619962 @default.
- W4220876852 cites W2058335181 @default.
- W4220876852 cites W2065144875 @default.
- W4220876852 cites W2069767813 @default.
- W4220876852 cites W2071465162 @default.
- W4220876852 cites W2074737103 @default.
- W4220876852 cites W2077745729 @default.
- W4220876852 cites W2084883027 @default.
- W4220876852 cites W2087741619 @default.
- W4220876852 cites W2090541228 @default.
- W4220876852 cites W2101195657 @default.
- W4220876852 cites W2105790712 @default.
- W4220876852 cites W2106992274 @default.
- W4220876852 cites W2111764708 @default.
- W4220876852 cites W2134918219 @default.
- W4220876852 cites W2144501135 @default.
- W4220876852 cites W2149153491 @default.
- W4220876852 cites W2160997238 @default.
- W4220876852 cites W2161765631 @default.
- W4220876852 cites W2165178639 @default.
- W4220876852 cites W2169679602 @default.
- W4220876852 cites W2253053879 @default.
- W4220876852 cites W2267905372 @default.
- W4220876852 cites W2304567270 @default.
- W4220876852 cites W2328937666 @default.
- W4220876852 cites W2346468322 @default.
- W4220876852 cites W2469276610 @default.
- W4220876852 cites W2546409538 @default.
- W4220876852 cites W2575182800 @default.
- W4220876852 cites W2791436252 @default.
- W4220876852 cites W2808152773 @default.
- W4220876852 cites W2866577345 @default.
- W4220876852 cites W2895116377 @default.
- W4220876852 cites W2901101683 @default.
- W4220876852 cites W2920887542 @default.
- W4220876852 cites W2979905735 @default.
- W4220876852 cites W2984278771 @default.
- W4220876852 cites W2989569229 @default.
- W4220876852 cites W3001363503 @default.
- W4220876852 cites W3004119871 @default.
- W4220876852 cites W3013161644 @default.
- W4220876852 cites W3014040934 @default.
- W4220876852 cites W3120642963 @default.
- W4220876852 cites W3123442693 @default.
- W4220876852 cites W3132145256 @default.
- W4220876852 cites W3135893678 @default.
- W4220876852 cites W3189106252 @default.
- W4220876852 cites W4205450559 @default.
- W4220876852 cites W4206664789 @default.
- W4220876852 doi "https://doi.org/10.1186/s12934-021-01729-6" @default.
- W4220876852 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35255900" @default.
- W4220876852 hasPublicationYear "2022" @default.
- W4220876852 type Work @default.
- W4220876852 citedByCount "10" @default.
- W4220876852 countsByYear W42208768522023 @default.
- W4220876852 crossrefType "journal-article" @default.
- W4220876852 hasAuthorship W4220876852A5014358648 @default.
- W4220876852 hasAuthorship W4220876852A5052829635 @default.
- W4220876852 hasAuthorship W4220876852A5061817773 @default.
- W4220876852 hasAuthorship W4220876852A5067666867 @default.
- W4220876852 hasAuthorship W4220876852A5075747124 @default.
- W4220876852 hasAuthorship W4220876852A5085236029 @default.
- W4220876852 hasAuthorship W4220876852A5086511480 @default.
- W4220876852 hasAuthorship W4220876852A5087006756 @default.
- W4220876852 hasBestOaLocation W42208768521 @default.
- W4220876852 hasConcept C100544194 @default.
- W4220876852 hasConcept C104317684 @default.
- W4220876852 hasConcept C181199279 @default.
- W4220876852 hasConcept C2776996202 @default.
- W4220876852 hasConcept C2780115692 @default.
- W4220876852 hasConcept C2780206646 @default.
- W4220876852 hasConcept C2780255968 @default.
- W4220876852 hasConcept C2780305828 @default.