FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4220886985> ?p ?o ?g. }

• W4220886985 endingPage "327" @default.
• W4220886985 startingPage "307" @default.
• W4220886985 abstract "Endothelial cell (EC) activity is essential for tissue regeneration in several (patho)physiological contexts. However, our capacity to deliver in vivo biomolecules capable of controlling EC fate is relatively limited. Here, we screened a library of microRNA (miR) mimics and identified 25 miRs capable of enhancing the survival of ECs exposed to ischemia-mimicking conditions. In vitro, we showed that miR-425-5p, one of the hits, was able to enhance EC survival and migration. In vivo, using a mouse Matrigel plug assay, we showed that ECs transfected with miR-425-5p displayed enhanced survival compared with scramble-transfected ECs. Mechanistically, we showed that miR-425-5p modulated the PTEN/PI3K/AKT pathway and inhibition of miR-425-5p target genes (DACH1, PTEN, RGS5, and VASH1) phenocopied the pro-survival. For the in vivo delivery of miR-425-5p, we modulated small extracellular vesicles (sEVs) with miR-425-5p and showed, in vitro, that miR-425-5p-modulated sEVs were (1) capable of enhancing the survival of ECs exposed to ischemia-mimic conditions, and (2) efficiently internalized by skin cells. Finally, using a streptozotocin-induced diabetic wound healing mouse model, we showed that, compared with miR-scrambled-modulated sEVs, topical administration of miR-425-5p-modulated sEVs significantly enhanced wound healing, a process mediated by enhanced vascularization and skin re-epithelialization. Endothelial cell (EC) activity is essential for tissue regeneration in several (patho)physiological contexts. However, our capacity to deliver in vivo biomolecules capable of controlling EC fate is relatively limited. Here, we screened a library of microRNA (miR) mimics and identified 25 miRs capable of enhancing the survival of ECs exposed to ischemia-mimicking conditions. In vitro, we showed that miR-425-5p, one of the hits, was able to enhance EC survival and migration. In vivo, using a mouse Matrigel plug assay, we showed that ECs transfected with miR-425-5p displayed enhanced survival compared with scramble-transfected ECs. Mechanistically, we showed that miR-425-5p modulated the PTEN/PI3K/AKT pathway and inhibition of miR-425-5p target genes (DACH1, PTEN, RGS5, and VASH1) phenocopied the pro-survival. For the in vivo delivery of miR-425-5p, we modulated small extracellular vesicles (sEVs) with miR-425-5p and showed, in vitro, that miR-425-5p-modulated sEVs were (1) capable of enhancing the survival of ECs exposed to ischemia-mimic conditions, and (2) efficiently internalized by skin cells. Finally, using a streptozotocin-induced diabetic wound healing mouse model, we showed that, compared with miR-scrambled-modulated sEVs, topical administration of miR-425-5p-modulated sEVs significantly enhanced wound healing, a process mediated by enhanced vascularization and skin re-epithelialization." @default.
• W4220886985 created "2022-04-03" @default.
• W4220886985 creator A5004922894 @default.
• W4220886985 creator A5007901058 @default.
• W4220886985 creator A5026141213 @default.
• W4220886985 creator A5038080240 @default.
• W4220886985 creator A5042166089 @default.
• W4220886985 creator A5043580735 @default.
• W4220886985 creator A5051198147 @default.
• W4220886985 creator A5058820378 @default.
• W4220886985 creator A5061036331 @default.
• W4220886985 creator A5062859592 @default.
• W4220886985 creator A5067622760 @default.
• W4220886985 creator A5068283752 @default.
• W4220886985 creator A5069577087 @default.
• W4220886985 creator A5076001987 @default.
• W4220886985 creator A5082230492 @default.
• W4220886985 creator A5083337259 @default.
• W4220886985 creator A5085935671 @default.
• W4220886985 creator A5087517683 @default.
• W4220886985 creator A5087750619 @default.
• W4220886985 date "2022-06-01" @default.
• W4220886985 modified "2023-10-16" @default.
• W4220886985 title "Extracellular vesicles enriched with an endothelial cell pro-survival microRNA affects skin tissue regeneration" @default.
• W4220886985 cites W1615617154 @default.
• W4220886985 cites W1877686344 @default.
• W4220886985 cites W1963906441 @default.
• W4220886985 cites W1966515321 @default.
• W4220886985 cites W1985718731 @default.
• W4220886985 cites W1989910375 @default.
• W4220886985 cites W2017739035 @default.
• W4220886985 cites W2024623214 @default.
• W4220886985 cites W2039551908 @default.
• W4220886985 cites W2048186369 @default.
• W4220886985 cites W2059843925 @default.
• W4220886985 cites W2062035530 @default.
• W4220886985 cites W2077475358 @default.
• W4220886985 cites W2077844244 @default.
• W4220886985 cites W2078554706 @default.
• W4220886985 cites W2104857427 @default.
• W4220886985 cites W2107277218 @default.
• W4220886985 cites W2110419512 @default.
• W4220886985 cites W2112804253 @default.
• W4220886985 cites W2128111546 @default.
• W4220886985 cites W2140026638 @default.
• W4220886985 cites W2142280970 @default.
• W4220886985 cites W2144767310 @default.
• W4220886985 cites W2169859308 @default.
• W4220886985 cites W2192080449 @default.
• W4220886985 cites W2230281891 @default.
• W4220886985 cites W2399602153 @default.
• W4220886985 cites W2521459783 @default.
• W4220886985 cites W2592332381 @default.
• W4220886985 cites W2611096724 @default.
• W4220886985 cites W2742785308 @default.
• W4220886985 cites W2759504685 @default.
• W4220886985 cites W2786514708 @default.
• W4220886985 cites W2790215486 @default.
• W4220886985 cites W2795438042 @default.
• W4220886985 cites W2797059394 @default.
• W4220886985 cites W2808115170 @default.
• W4220886985 cites W2885279306 @default.
• W4220886985 cites W2892397780 @default.
• W4220886985 cites W2900798880 @default.
• W4220886985 cites W2902014035 @default.
• W4220886985 cites W2902282736 @default.
• W4220886985 cites W2904628185 @default.
• W4220886985 cites W2954356826 @default.
• W4220886985 cites W2965527105 @default.
• W4220886985 cites W2980006108 @default.
• W4220886985 cites W2981029611 @default.
• W4220886985 cites W3005104290 @default.
• W4220886985 cites W3014252919 @default.
• W4220886985 cites W3014432468 @default.
• W4220886985 cites W3032105658 @default.
• W4220886985 cites W3047645759 @default.
• W4220886985 cites W3048723046 @default.
• W4220886985 cites W3081273594 @default.
• W4220886985 cites W3092156279 @default.
• W4220886985 cites W3093677713 @default.
• W4220886985 cites W3175628958 @default.
• W4220886985 cites W3189262009 @default.
• W4220886985 cites W3189962908 @default.
• W4220886985 doi "https://doi.org/10.1016/j.omtn.2022.03.018" @default.
• W4220886985 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35474734" @default.
• W4220886985 hasPublicationYear "2022" @default.
• W4220886985 type Work @default.
• W4220886985 citedByCount "4" @default.
• W4220886985 countsByYear W42208869852022 @default.
• W4220886985 countsByYear W42208869852023 @default.
• W4220886985 crossrefType "journal-article" @default.
• W4220886985 hasAuthorship W4220886985A5004922894 @default.
• W4220886985 hasAuthorship W4220886985A5007901058 @default.
• W4220886985 hasAuthorship W4220886985A5026141213 @default.
• W4220886985 hasAuthorship W4220886985A5038080240 @default.
• W4220886985 hasAuthorship W4220886985A5042166089 @default.
• W4220886985 hasAuthorship W4220886985A5043580735 @default.
• W4220886985 hasAuthorship W4220886985A5051198147 @default.

• next