Matches in SemOpenAlex for { <https://semopenalex.org/work/W4220900362> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W4220900362 abstract "During development cell fates are specified by tightly controlled gene expression programs. PBX TALE transcription factors control gene regulatory networks (GRN) that direct vertebrate tissue patterning and organ morphogenesis. How PBX1/2 proteins acquire context-specific functions, despite widespread embryonic expression of Pbx1/2, remains elusive. In mouse limb buds, mesenchymal-specific loss of PBX1/2 or of the transcriptional regulator HAND2 results in similar phenotypes, suggesting that PBX1/2- and HAND2-dependent programs converge to control limb development. To investigate this scenario, we combined tissue-specific and temporally-controlled mutagenesis with multi-omics approaches using the murine hindlimb bud as a model. We reconstructed a GRN collaboratively directed by PBX1/2 and HAND2, demonstrating that Pbx1-Hand2 genetically interact in vivo during hindlimb patterning, with PBX1 concomitantly acting as an upstream regulator of Hand2. At organismal-level resolution the GRN is active within restricted subsets of posterior-proximal hindlimb mesenchymal cells, wherein Pbx1/2 and Hand2 are co-expressed with their target genes. Genome-wide profiling of PBX1 binding across multiple tissues further revealed that HAND2 selects a subset of PBX-bound regions to impart limb patterning functionality. This research elucidates mechanisms underlying limb bud-specific functions by PBX1/2, while informing general principles by which promiscuous transcription factors cooperate with select cofactors to instruct distinct developmental programs." @default.
- W4220900362 created "2022-04-03" @default.
- W4220900362 creator A5001390341 @default.
- W4220900362 creator A5002159730 @default.
- W4220900362 creator A5004075942 @default.
- W4220900362 creator A5011677640 @default.
- W4220900362 creator A5012615456 @default.
- W4220900362 creator A5012731672 @default.
- W4220900362 creator A5013978553 @default.
- W4220900362 creator A5016288715 @default.
- W4220900362 creator A5016891383 @default.
- W4220900362 creator A5020096278 @default.
- W4220900362 creator A5023809322 @default.
- W4220900362 creator A5031851719 @default.
- W4220900362 creator A5034972348 @default.
- W4220900362 creator A5041405111 @default.
- W4220900362 creator A5056518897 @default.
- W4220900362 creator A5062045808 @default.
- W4220900362 creator A5080587435 @default.
- W4220900362 date "2022-03-09" @default.
- W4220900362 modified "2023-10-14" @default.
- W4220900362 title "A spatio-temporally constrained gene regulatory network directed by PBX1/2 acquires limb patterning specificity via HAND2" @default.
- W4220900362 doi "https://doi.org/10.1101/2022.03.08.483529" @default.
- W4220900362 hasPublicationYear "2022" @default.
- W4220900362 type Work @default.
- W4220900362 citedByCount "0" @default.
- W4220900362 crossrefType "posted-content" @default.
- W4220900362 hasAuthorship W4220900362A5001390341 @default.
- W4220900362 hasAuthorship W4220900362A5002159730 @default.
- W4220900362 hasAuthorship W4220900362A5004075942 @default.
- W4220900362 hasAuthorship W4220900362A5011677640 @default.
- W4220900362 hasAuthorship W4220900362A5012615456 @default.
- W4220900362 hasAuthorship W4220900362A5012731672 @default.
- W4220900362 hasAuthorship W4220900362A5013978553 @default.
- W4220900362 hasAuthorship W4220900362A5016288715 @default.
- W4220900362 hasAuthorship W4220900362A5016891383 @default.
- W4220900362 hasAuthorship W4220900362A5020096278 @default.
- W4220900362 hasAuthorship W4220900362A5023809322 @default.
- W4220900362 hasAuthorship W4220900362A5031851719 @default.
- W4220900362 hasAuthorship W4220900362A5034972348 @default.
- W4220900362 hasAuthorship W4220900362A5041405111 @default.
- W4220900362 hasAuthorship W4220900362A5056518897 @default.
- W4220900362 hasAuthorship W4220900362A5062045808 @default.
- W4220900362 hasAuthorship W4220900362A5080587435 @default.
- W4220900362 hasBestOaLocation W42209003621 @default.
- W4220900362 hasConcept C104317684 @default.
- W4220900362 hasConcept C127716648 @default.
- W4220900362 hasConcept C145103041 @default.
- W4220900362 hasConcept C150194340 @default.
- W4220900362 hasConcept C165864922 @default.
- W4220900362 hasConcept C25770073 @default.
- W4220900362 hasConcept C54355233 @default.
- W4220900362 hasConcept C67339327 @default.
- W4220900362 hasConcept C6929976 @default.
- W4220900362 hasConcept C86339819 @default.
- W4220900362 hasConcept C86370654 @default.
- W4220900362 hasConcept C86803240 @default.
- W4220900362 hasConcept C95444343 @default.
- W4220900362 hasConceptScore W4220900362C104317684 @default.
- W4220900362 hasConceptScore W4220900362C127716648 @default.
- W4220900362 hasConceptScore W4220900362C145103041 @default.
- W4220900362 hasConceptScore W4220900362C150194340 @default.
- W4220900362 hasConceptScore W4220900362C165864922 @default.
- W4220900362 hasConceptScore W4220900362C25770073 @default.
- W4220900362 hasConceptScore W4220900362C54355233 @default.
- W4220900362 hasConceptScore W4220900362C67339327 @default.
- W4220900362 hasConceptScore W4220900362C6929976 @default.
- W4220900362 hasConceptScore W4220900362C86339819 @default.
- W4220900362 hasConceptScore W4220900362C86370654 @default.
- W4220900362 hasConceptScore W4220900362C86803240 @default.
- W4220900362 hasConceptScore W4220900362C95444343 @default.
- W4220900362 hasLocation W42209003621 @default.
- W4220900362 hasLocation W42209003622 @default.
- W4220900362 hasOpenAccess W4220900362 @default.
- W4220900362 hasPrimaryLocation W42209003621 @default.
- W4220900362 hasRelatedWork W31778427 @default.
- W4220900362 hasRelatedWork W32192003 @default.
- W4220900362 hasRelatedWork W33125175 @default.
- W4220900362 hasRelatedWork W33133445 @default.
- W4220900362 hasRelatedWork W40671563 @default.
- W4220900362 hasRelatedWork W4134988 @default.
- W4220900362 hasRelatedWork W41584537 @default.
- W4220900362 hasRelatedWork W4893628 @default.
- W4220900362 hasRelatedWork W6047463 @default.
- W4220900362 hasRelatedWork W8014543 @default.
- W4220900362 isParatext "false" @default.
- W4220900362 isRetracted "false" @default.
- W4220900362 workType "article" @default.