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- W4220904119 abstract "Micropatterned suspension culture creates consistently sized and shaped cell aggregates but has not produced organotypic structures from stable cells, thus restricting its use in accurate disease modeling. Here, we show that organotypic structure is achieved in hybrid suspension culture via supplementation of soluble extracellular matrix (ECM). We created a viable lung organoid from epithelial, endothelial, and fibroblast human stable cell lines in suspension culture. We demonstrate the importance of soluble ECM in organotypic patterning with the emergence of lumen-like structures with airspace showing feasible gas exchange units, formation of branching, perfusable vasculature, and long-term 70-day maintenance of lumen structure. Our results show a dependent relationship between enhanced fibronectin fibril assembly and the incorporation of ECM in the organoid. We successfully applied this technology in modeling lung fibrosis via bleomycin induction and test a potential antifibrotic drug in vitro while maintaining fundamental cell-cell interactions in lung tissue. Our human fluorescent lung organoid (hFLO) model represents features of pulmonary fibrosis which were ameliorated by fasudil treatment. We also demonstrate a 3D culture method with potential of creating organoids from mature cells, thus opening avenues for disease modeling and regenerative medicine, enhancing understanding of lung cell biology in health and lung disease." @default.
- W4220904119 created "2022-04-03" @default.
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- W4220904119 date "2022-04-01" @default.
- W4220904119 modified "2023-10-06" @default.
- W4220904119 title "Soluble ECM promotes organotypic formation in lung alveolar model" @default.
- W4220904119 cites W1579204932 @default.
- W4220904119 cites W1938547050 @default.
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- W4220904119 cites W1966307963 @default.
- W4220904119 cites W1967827717 @default.
- W4220904119 cites W1968107959 @default.
- W4220904119 cites W1976115786 @default.
- W4220904119 cites W1982159478 @default.
- W4220904119 cites W1993559550 @default.
- W4220904119 cites W1994863837 @default.
- W4220904119 cites W1997508706 @default.
- W4220904119 cites W2003706739 @default.
- W4220904119 cites W2006458374 @default.
- W4220904119 cites W2030378746 @default.
- W4220904119 cites W2031943423 @default.
- W4220904119 cites W2036200411 @default.
- W4220904119 cites W2036937264 @default.
- W4220904119 cites W2037199116 @default.
- W4220904119 cites W2039403501 @default.
- W4220904119 cites W2040621903 @default.
- W4220904119 cites W2045295282 @default.
- W4220904119 cites W2046402273 @default.
- W4220904119 cites W2049410632 @default.
- W4220904119 cites W2051354090 @default.
- W4220904119 cites W2051529247 @default.
- W4220904119 cites W2051772841 @default.
- W4220904119 cites W2052700237 @default.
- W4220904119 cites W2060838925 @default.
- W4220904119 cites W2064259509 @default.
- W4220904119 cites W2065977597 @default.
- W4220904119 cites W2067043202 @default.
- W4220904119 cites W2073353515 @default.
- W4220904119 cites W2089798586 @default.
- W4220904119 cites W2090944837 @default.
- W4220904119 cites W2100628426 @default.
- W4220904119 cites W2100808624 @default.
- W4220904119 cites W2103759210 @default.
- W4220904119 cites W2104876248 @default.
- W4220904119 cites W2109401564 @default.
- W4220904119 cites W2112664126 @default.
- W4220904119 cites W2114229735 @default.
- W4220904119 cites W2115219176 @default.
- W4220904119 cites W2124559417 @default.
- W4220904119 cites W2126432370 @default.
- W4220904119 cites W2130410032 @default.
- W4220904119 cites W2130522035 @default.
- W4220904119 cites W2132566055 @default.
- W4220904119 cites W2138094019 @default.
- W4220904119 cites W2140923971 @default.
- W4220904119 cites W2141235785 @default.
- W4220904119 cites W2143491429 @default.
- W4220904119 cites W2160522284 @default.
- W4220904119 cites W2163526088 @default.
- W4220904119 cites W2168215545 @default.
- W4220904119 cites W2168854899 @default.
- W4220904119 cites W2229805806 @default.
- W4220904119 cites W2405984650 @default.
- W4220904119 cites W2409253424 @default.
- W4220904119 cites W2415623504 @default.
- W4220904119 cites W2430007335 @default.
- W4220904119 cites W2441337908 @default.
- W4220904119 cites W2524612783 @default.
- W4220904119 cites W2563494429 @default.
- W4220904119 cites W2580449060 @default.
- W4220904119 cites W2609587333 @default.
- W4220904119 cites W2620579988 @default.
- W4220904119 cites W2735660862 @default.
- W4220904119 cites W2755989260 @default.
- W4220904119 cites W2768709220 @default.
- W4220904119 cites W2773729171 @default.
- W4220904119 cites W2774399208 @default.