FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4220908738> ?p ?o ?g. }

• W4220908738 abstract "Abstract The SARS-CoV-1 spike glycoprotein contains a fusion peptide (FP) segment that mediates fusion of the viral and host cell membranes. Calcium ions are thought to position the FP optimally for membrane insertion by interacting with negatively charged residues in this segment (E801, D802, D812, E821, D825, and D830); however, which residues bind to calcium and in what combinations supportive of membrane insertion are unknown. Using biological assays and molecular dynamics studies, we have determined the functional configurations of FP-Ca +2 binding which promote membrane insertion. We first mutated the negatively charged residues in the SARS CoV-1 FP to assay their role in cell entry and syncytia formation, finding that charge loss in the D802A or D830A mutants reduced syncytia formation and pseudoparticle transduction. Interestingly, the D812A mutation led to increased pseudoparticle transduction, indicating the Ca 2+ effect depends on binding at specific FP sites. To interpret mechanistically these results and learn how specific modes of FP-Ca 2+ binding modulate membrane insertion, we performed molecular dynamics simulations. Preferred residue pairs for Ca 2+ binding were identified (E801/D802; E801/D830; D812/E821) which promote FP membrane insertion. In contrast, binding to residues E821/D825 inhibited FP membrane insertion, which is also supported by our biological assays. Our findings show that Ca 2+ binding to SARS-CoV-1 FP residue pairs E801/D802 and D812/E821 facilitates membrane insertion, whereas binding to the E801/D802 and D821/D825 pairs is detrimental. These conclusions provide an improved and nuanced mechanistic understanding of calcium binding modes to FP residues and their dynamic effects on host cell entry." @default.
• W4220908738 created "2022-04-03" @default.
• W4220908738 creator A5014039584 @default.
• W4220908738 creator A5014370221 @default.
• W4220908738 creator A5014643846 @default.
• W4220908738 creator A5047815097 @default.
• W4220908738 creator A5058105711 @default.
• W4220908738 creator A5060527670 @default.
• W4220908738 creator A5074820527 @default.
• W4220908738 creator A5078607944 @default.
• W4220908738 creator A5080767725 @default.
• W4220908738 date "2022-03-04" @default.
• W4220908738 modified "2023-10-16" @default.
• W4220908738 title "It Takes Two: Bimodal interactions between the SARS-CoV-1 fusion peptide and Ca2+ ions promote membrane insertion and viral entry" @default.
• W4220908738 cites W1544561280 @default.
• W4220908738 cites W1965531501 @default.
• W4220908738 cites W1967293609 @default.
• W4220908738 cites W1970357363 @default.
• W4220908738 cites W1978623713 @default.
• W4220908738 cites W1987327282 @default.
• W4220908738 cites W1990985479 @default.
• W4220908738 cites W1994915168 @default.
• W4220908738 cites W2029667189 @default.
• W4220908738 cites W2035687084 @default.
• W4220908738 cites W2037987211 @default.
• W4220908738 cites W2053837540 @default.
• W4220908738 cites W2079988775 @default.
• W4220908738 cites W2107920732 @default.
• W4220908738 cites W2112147913 @default.
• W4220908738 cites W2140603934 @default.
• W4220908738 cites W2140674451 @default.
• W4220908738 cites W2146374988 @default.
• W4220908738 cites W2150981663 @default.
• W4220908738 cites W2155728582 @default.
• W4220908738 cites W2158118659 @default.
• W4220908738 cites W2330799739 @default.
• W4220908738 cites W2470646526 @default.
• W4220908738 cites W2555870966 @default.
• W4220908738 cites W2564443324 @default.
• W4220908738 cites W2766494877 @default.
• W4220908738 cites W2949223833 @default.
• W4220908738 cites W2990108599 @default.
• W4220908738 cites W3003217347 @default.
• W4220908738 cites W3004280078 @default.
• W4220908738 cites W3014912119 @default.
• W4220908738 cites W3015512747 @default.
• W4220908738 cites W3039901154 @default.
• W4220908738 cites W3042332408 @default.
• W4220908738 cites W3043431476 @default.
• W4220908738 cites W3043610128 @default.
• W4220908738 cites W3107228882 @default.
• W4220908738 cites W3120823005 @default.
• W4220908738 cites W3130543912 @default.
• W4220908738 cites W3137974527 @default.
• W4220908738 cites W3178247263 @default.
• W4220908738 cites W3186939038 @default.
• W4220908738 cites W3189832346 @default.
• W4220908738 cites W3196973066 @default.
• W4220908738 cites W4200181709 @default.
• W4220908738 cites W4223643516 @default.
• W4220908738 cites W4298832655 @default.
• W4220908738 doi "https://doi.org/10.1101/2022.03.03.482731" @default.
• W4220908738 hasPublicationYear "2022" @default.
• W4220908738 type Work @default.
• W4220908738 citedByCount "0" @default.
• W4220908738 crossrefType "posted-content" @default.
• W4220908738 hasAuthorship W4220908738A5014039584 @default.
• W4220908738 hasAuthorship W4220908738A5014370221 @default.
• W4220908738 hasAuthorship W4220908738A5014643846 @default.
• W4220908738 hasAuthorship W4220908738A5047815097 @default.
• W4220908738 hasAuthorship W4220908738A5058105711 @default.
• W4220908738 hasAuthorship W4220908738A5060527670 @default.
• W4220908738 hasAuthorship W4220908738A5074820527 @default.
• W4220908738 hasAuthorship W4220908738A5078607944 @default.
• W4220908738 hasAuthorship W4220908738A5080767725 @default.
• W4220908738 hasBestOaLocation W42209087381 @default.
• W4220908738 hasConcept C103038307 @default.
• W4220908738 hasConcept C104317684 @default.
• W4220908738 hasConcept C107824862 @default.
• W4220908738 hasConcept C12554922 @default.
• W4220908738 hasConcept C143065580 @default.
• W4220908738 hasConcept C15152581 @default.
• W4220908738 hasConcept C185592680 @default.
• W4220908738 hasConcept C2778106830 @default.
• W4220908738 hasConcept C2779281246 @default.
• W4220908738 hasConcept C41625074 @default.
• W4220908738 hasConcept C55493867 @default.
• W4220908738 hasConcept C86803240 @default.
• W4220908738 hasConcept C95444343 @default.
• W4220908738 hasConceptScore W4220908738C103038307 @default.
• W4220908738 hasConceptScore W4220908738C104317684 @default.
• W4220908738 hasConceptScore W4220908738C107824862 @default.
• W4220908738 hasConceptScore W4220908738C12554922 @default.
• W4220908738 hasConceptScore W4220908738C143065580 @default.
• W4220908738 hasConceptScore W4220908738C15152581 @default.
• W4220908738 hasConceptScore W4220908738C185592680 @default.
• W4220908738 hasConceptScore W4220908738C2778106830 @default.
• W4220908738 hasConceptScore W4220908738C2779281246 @default.
• W4220908738 hasConceptScore W4220908738C41625074 @default.
• W4220908738 hasConceptScore W4220908738C55493867 @default.

• next