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- W4220921625 abstract "Abstract DNA supercoiling has emerged as a major contributor to gene regulation in bacteria. The impact of DNA supercoiling on transcription dynamics in eukaryotes is less clear. Here, using single-molecule dual-color RNA imaging in budding yeast, we show that transcriptional bursting of the divergent and tandem GAL genes is coupled. Upon topoisomerase degradation, supercoils that buildup from transcription inhibit subsequent transcription at neighboring genes, thereby reducing their simultaneous bursting. GAL gene transcription is inhibited more by negative than by positive supercoiling accumulation. Unlike bacteria, wildtype yeast has sufficient topoisomerase levels to minimize inhibition from supercoils at adjacent genes. Overall, we discover fundamental differences in supercoiling-mediated gene regulation between bacteria and yeast and show that rapid supercoiling release in eukaryotes ensures proper gene expression of neighboring genes. One sentence summary Transcription causes twisting of the DNA double helix, which can inhibit transcription of adjacent genes." @default.
- W4220921625 created "2022-04-03" @default.
- W4220921625 creator A5033153166 @default.
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- W4220921625 date "2022-03-04" @default.
- W4220921625 modified "2023-10-17" @default.
- W4220921625 title "DNA supercoiling restricts the transcriptional bursting of neighboring eukaryotic genes" @default.
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- W4220921625 doi "https://doi.org/10.1101/2022.03.04.482969" @default.
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