Matches in SemOpenAlex for { <https://semopenalex.org/work/W4220922403> ?p ?o ?g. }
- W4220922403 abstract "Abstract Background To develop an effective malaria vector intervention method in forested international border regions within the Greater Mekong Subregion (GMS), more in-depth studies should be conducted on local Anopheles species composition and bionomic features. There is a paucity of comprehensive surveys of biodiversity integrating morphological and molecular species identification conducted within the border of Laos and Cambodia. Methods A total of 2394 adult mosquitoes were trapped in the Cambodia–Laos border region. We first performed morphological identification of Anopheles mosquitoes and subsequently performed molecular identification using 412 recombinant DNA–internal transcribed spacer 2 (rDNA-ITS2) and 391 mitochondrial DNA–cytochrome c oxidase subunit 2 ( mtDNA-COII ) sequences. The molecular and morphological identification results were compared, and phylogenetic analysis of rDNA-ITS2 and mtDNA-COII was conducted for the sequence divergence among species. Results Thirteen distinct species of Anopheles were molecularly identified in a 26,415 km 2 border region in Siem Pang (Cambodia) and Pathoomphone (Laos). According to the comparisons of morphological and molecular identity, the interpretation of local species composition for dominant species in the Cambodia–Laos border ( An. dirus , An. maculatus , An. philippinensis , An. kochi and An. sinensis ) achieved the highest accuracy of morphological identification, from 98.37 to 100%. In contrast, the other species which were molecularly identified were less frequently identified correctly (0–58.3%) by morphological methods. The average rDNA-ITS2 and mtDNA-COII interspecific divergence was respectively 318 times and 15 times higher than their average intraspecific divergence. The barcoding gap ranged from 0.042 to 0.193 for rDNA-ITS2, and from 0.033 to 0.047 for mtDNA-COII . Conclusions The Cambodia–Laos border hosts a high diversity of Anopheles species. The morphological identification of Anopheles species provides higher accuracy for dominant species than for other species. Molecular methods combined with morphological analysis to determine species composition, population dynamics and bionomic characteristics can facilitate a better understanding of the factors driving malaria transmission and the effects of interventions, and can aid in achieving the goal of eliminating malaria. Graphical Abstract" @default.
- W4220922403 created "2022-04-03" @default.
- W4220922403 creator A5005315269 @default.
- W4220922403 creator A5036547741 @default.
- W4220922403 creator A5038013807 @default.
- W4220922403 creator A5050388216 @default.
- W4220922403 creator A5065930365 @default.
- W4220922403 creator A5070218441 @default.
- W4220922403 creator A5086546519 @default.
- W4220922403 creator A5087814879 @default.
- W4220922403 date "2022-03-18" @default.
- W4220922403 modified "2023-10-16" @default.
- W4220922403 title "Morphological and molecular identification reveals a high diversity of Anopheles species in the forest region of the Cambodia–Laos border" @default.
- W4220922403 cites W106715878 @default.
- W4220922403 cites W1499098859 @default.
- W4220922403 cites W1552798925 @default.
- W4220922403 cites W168952994 @default.
- W4220922403 cites W1749239895 @default.
- W4220922403 cites W1938501462 @default.
- W4220922403 cites W1965204119 @default.
- W4220922403 cites W1973675571 @default.
- W4220922403 cites W1974516011 @default.
- W4220922403 cites W1976035546 @default.
- W4220922403 cites W1981617950 @default.
- W4220922403 cites W1986579416 @default.
- W4220922403 cites W1988113894 @default.
- W4220922403 cites W1988117836 @default.
- W4220922403 cites W1998347450 @default.
- W4220922403 cites W1998364854 @default.
- W4220922403 cites W2005040560 @default.
- W4220922403 cites W2009673514 @default.
- W4220922403 cites W2015190302 @default.
- W4220922403 cites W2015648530 @default.
- W4220922403 cites W2022038591 @default.
- W4220922403 cites W2023610786 @default.
- W4220922403 cites W2025709436 @default.
- W4220922403 cites W2031488007 @default.
- W4220922403 cites W2036848717 @default.
- W4220922403 cites W2052757069 @default.
- W4220922403 cites W2052771068 @default.
- W4220922403 cites W2065461553 @default.
- W4220922403 cites W2065973577 @default.
- W4220922403 cites W2075344966 @default.
- W4220922403 cites W207945776 @default.
- W4220922403 cites W2079683145 @default.
- W4220922403 cites W2082040429 @default.
- W4220922403 cites W2084833023 @default.
- W4220922403 cites W2088824647 @default.
- W4220922403 cites W2089804680 @default.
- W4220922403 cites W2096420865 @default.
- W4220922403 cites W210045683 @default.
- W4220922403 cites W2108221956 @default.
- W4220922403 cites W2121149783 @default.
- W4220922403 cites W2121677860 @default.
- W4220922403 cites W2124200821 @default.
- W4220922403 cites W2125486032 @default.
- W4220922403 cites W2130416912 @default.
- W4220922403 cites W2131641279 @default.
- W4220922403 cites W2135995652 @default.
- W4220922403 cites W2137288898 @default.
- W4220922403 cites W2151525582 @default.
- W4220922403 cites W2154845780 @default.
- W4220922403 cites W2158427926 @default.
- W4220922403 cites W2159701849 @default.
- W4220922403 cites W2162714623 @default.
- W4220922403 cites W2164039894 @default.
- W4220922403 cites W2164532325 @default.
- W4220922403 cites W2167946670 @default.
- W4220922403 cites W2173581379 @default.
- W4220922403 cites W2254914524 @default.
- W4220922403 cites W2294544759 @default.
- W4220922403 cites W2322935909 @default.
- W4220922403 cites W2332419461 @default.
- W4220922403 cites W2389977812 @default.
- W4220922403 cites W2402806192 @default.
- W4220922403 cites W2405354194 @default.
- W4220922403 cites W2408701468 @default.
- W4220922403 cites W2419606266 @default.
- W4220922403 cites W2432877378 @default.
- W4220922403 cites W2438287964 @default.
- W4220922403 cites W2513835608 @default.
- W4220922403 cites W2531728433 @default.
- W4220922403 cites W2603738034 @default.
- W4220922403 cites W2604711943 @default.
- W4220922403 cites W2608727333 @default.
- W4220922403 cites W2612906983 @default.
- W4220922403 cites W2621128804 @default.
- W4220922403 cites W2741193251 @default.
- W4220922403 cites W2742919462 @default.
- W4220922403 cites W2752313752 @default.
- W4220922403 cites W2770581499 @default.
- W4220922403 cites W2799524357 @default.
- W4220922403 cites W2933419141 @default.
- W4220922403 cites W2945992040 @default.
- W4220922403 cites W2961018688 @default.
- W4220922403 cites W2966751289 @default.
- W4220922403 cites W2967151732 @default.
- W4220922403 cites W2968822389 @default.
- W4220922403 cites W2995570892 @default.
- W4220922403 cites W3045791729 @default.